LOCUS       sequence-350808-        9341 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3rd gen lentiviral Gateway destination vector, expression, CMV 
            promoter, Blast .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9341)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 9341)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pLenti CMV Blast DEST (706-1)
FEATURES             Location/Qualifiers
     source          1..9341
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(63..83)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        68..397
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      248..383
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        445..492
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             511..909
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             996..1229
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1301..1422
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1338..1357)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1392..1411
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1462..1597
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1617..1636)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1618..1636)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1646..1663)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1646..1662)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1655..1677)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1804..2259
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1891..1910)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2101..2122
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2285..2389
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2390..2458,2459..3250)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2608..2627)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3421..4009
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3910..3929
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4163..4180
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4297..4318
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4333..4338,4339..4356,4357..4363)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4371..4387
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4376..4398
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4395..4411
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4395..4411
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4430..4450
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4432..4450
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4478..4704
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4705..4885
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4932..5057
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5550..5783
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5968..6012
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6279..6395
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        6418..6721
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6722..6925
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6876..6896
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6922..6946
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    7030..7154
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(7179..7184,7185..7202,7203..7209)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             7263..7922
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(7329..7348)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             8264..8569
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     8448..8467
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(8610..8734)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    join(8765..9341,1..12)
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8818..8838)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9236..9247)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ccgcctcccc gcctggaatt ctgcagatat ccggttagta atgagtttgg aattaattct
       61 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
      121 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
      181 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
      241 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
      301 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta
      361 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc
      421 cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta tatcggcata
      481 gtataatacg acaaggtgag gaactaaacc atggccaagc ctttgtctca agaagaatcc
      541 accctcattg aaagagcaac ggctacaatc aacagcatcc ccatctctga agactacagc
      601 gtcgccagcg cagctctctc tagcgacggc cgcatcttca ctggtgtcaa tgtatatcat
      661 tttactgggg gaccttgtgc agaactcgtg gtgctgggca ctgctgctgc tgcggcagct
      721 ggcaacctga cttgtatcgt cgcgatcgga aatgagaaca ggggcatctt gagcccctgc
      781 ggacggtgcc gacaggtgct tctcgatctg catcctggga tcaaagccat agtgaaggac
      841 agtgatggac agccgacggc agttgggatt cgtgaattgc tgccctctgg ttatgtgtgg
      901 gagggctaag cacaattcga gctcggtacc tttaagacca atgacttaca aggcagctgt
      961 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctagctc actcccaacg
     1021 aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     1081 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     1141 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     1201 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta
     1261 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta
     1321 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     1381 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct
     1441 atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     1501 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     1561 ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct
     1621 atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     1681 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     1741 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     1801 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     1861 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     1921 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     1981 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     2041 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     2101 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     2161 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     2221 ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa
     2281 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     2341 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     2401 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     2461 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     2521 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     2581 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     2641 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     2701 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     2761 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     2821 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     2881 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     2941 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     3001 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     3061 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     3121 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     3181 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     3241 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     3301 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     3361 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     3421 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     3481 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     3541 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     3601 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     3661 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     3721 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     3781 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     3841 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     3901 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     3961 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     4021 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     4081 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     4141 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     4201 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     4261 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     4321 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     4381 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     4441 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     4501 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     4561 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     4621 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     4681 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     4741 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     4801 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     4861 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     4921 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     4981 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     5041 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     5101 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     5161 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     5221 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     5281 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     5341 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     5401 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     5461 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     5521 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     5581 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     5641 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     5701 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     5761 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     5821 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     5881 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     5941 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     6001 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     6061 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     6121 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     6181 ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc
     6241 attcgattag tgaacggatc tcgacggtat cggttaactt ttaaaagaaa aggggggatt
     6301 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
     6361 gaattacaaa aacaaattac aaaattcaaa attttatcga taagcttggg agttccgcgt
     6421 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
     6481 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
     6541 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
     6601 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
     6661 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
     6721 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
     6781 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
     6841 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
     6901 gtgggaggtc tatataagca gagctcgttt agtgaaccgt cagatcgcct ggagacgcca
     6961 tccacgctgt tttgacctcc atagaagaca ccgactctag tccagtgtgg tggaattctg
     7021 cagatatcaa caagtttgta caaaaaagct gaacgagaaa cgtaaaatga tataaatatc
     7081 aatatattaa attagatttt gcataaaaaa cagactacat aatactgtaa aacacaacat
     7141 atccagtcac tatggcggcc gcattaggca ccccaggctt tacactttat gcttccggct
     7201 cgtataatgt gtggattttg agttaggatc cgtcgagatt ttcaggagct aaggaagcta
     7261 aaatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg catcgtaaag
     7321 aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc gttcagctgg
     7381 atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat ccggccttta
     7441 ttcacattct tgcccgcctg atgaatgctc atccggaatt ccgtatggca atgaaagacg
     7501 gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat gagcaaactg
     7561 aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt ctacacatat
     7621 attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa gggtttattg
     7681 agaatatgtt tttcgtctca gccaatccct gggtgagttt caccagtttt gatttaaacg
     7741 tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat tatacgcaag
     7801 gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtttgt gatggcttcc
     7861 atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag ggcggggcgt
     7921 aaagatctgg atccggctta ctaaaagcca gataacagta tgcgtatttg cgcgctgatt
     7981 tttgcggtat aagaatatat actgatatgt atacccgaag tatgtcaaaa agaggtatgc
     8041 tatgaagcag cgtattacag tgacagttga cagcgacagc tatcagttgc tcaaggcata
     8101 tatgatgtca atatctccgg tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg
     8161 cgtgccgaac gctggaaagc ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt
     8221 gaaatgaacg gctcttttgc tgacgagaac aggggctggt gaaatgcagt ttaaggttta
     8281 cacctataaa agagagagcc gttatcgtct gtttgtggat gtacagagtg atattattga
     8341 cacgcccggg cgacggatgg tgatccccct ggccagtgca cgtctgctgt cagataaagt
     8401 ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa agctggcgca tgatgaccac
     8461 cgatatggcc agtgtgccgg tctccgttat cggggaagaa gtggctgatc tcagccaccg
     8521 cgaaaatgac atcaaaaacg ccattaacct gatgttctgg ggaatataaa tgtcaggctc
     8581 ccttatacac agccagtctg caggtcgacc atagtgactg gatatgttgt gttttacagt
     8641 attatgtagt ctgtttttta tgcaaaatct aatttaatat attgatattt atatcatttt
     8701 acgtttctcg ttcagctttc ttgtacaaag tggttgatat ccagcacagt ggcggccgct
     8761 cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     8821 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     8881 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     8941 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     9001 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     9061 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     9121 gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct
     9181 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     9241 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     9301 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg g
//
