LOCUS       sequence-350817-        9745 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9745)
  AUTHORS   Kawaguchi Y, Kovacs JJ, McLaurin A, Vance JM, Ito A, Yao TP
  TITLE     The deacetylase HDAC6 regulates aggresome formation and cell 
            viability in response to misfolded protein stress.
  JOURNAL   Cell. 2003 Dec 12. 115(6):727-38.
  PUBMED    14675537
REFERENCE   2  (bases 1 to 9745)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2003 
            Dec 12. 115(6):727-38."
COMMENT     Sequence Label: pcDNA-HDAC6-FLAG
FEATURES             Location/Qualifiers
     source          1..9745
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(2..21)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     248..268
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(277..705)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(387..408)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     599..618
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    751..975
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     964..981
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     promoter        1001..1019
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1001..1018
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             complement(1038..1061)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     intron          4827..5399
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(5435..5454)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5436..5454)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(5499..5702)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(5528..5548)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        5703..6082
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     6254..6273
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        6348..6452
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6453..6521,6522..7313)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6671..6690)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7484..8072
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7973..7992
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8226..8243
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8360..8381
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8396..8401,8402..8419,8420..8426)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8434..8450
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8439..8461
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8458..8474
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8458..8474
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    8523..8644
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8534..8553)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8588..8607
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(8818..9612)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8929..8948)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     9539..9558
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(join(9679..9745,1..263))
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(9693..9745,1..83)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 gcctcggcct ctgcataaat aaaaaaaatt agtcagccat ggggcggaga atgggcggaa
       61 ctgggcggag ttaggggcgg gatgggcgga gttaggggcg ggactatggt tgctgactaa
      121 ttgagatgca tgctttgcat acttctgcct gctggggagc ctggggactt tccacacctg
      181 gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga gcctggggac
      241 tttccacacc ctaactgaca cacattccac agaattaatt cgcgttaaat ttttgttaaa
      301 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat
      361 agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg
      421 tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac
      481 catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta
      541 aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag
      601 ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg
      661 taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtgggga taccccctag
      721 agccccagct ggttctttcc gcctcagaag ccatagagcc caccgcatcc ccagcatgcc
      781 tgctattgtc ttcccaatcc tcccccttgc tgtcctgccc caccccaccc cccagaatag
      841 aatgacacct actcagacaa tgcgatgcaa tttcctcatt ttattaggaa aggacagtgg
      901 gagtggcacc ttccagggtc aaggaaggca cgggggaggg gcaaacaaca gatggctggc
      961 aactagaagg cacagtcgag gctgatcagc gagctctagc atttaggtga cactatagaa
     1021 tagggccctc tagtttattt atcatcatca tctttataat ccccgtgtgg gtggggcata
     1081 tcctccccaa acttgttctg gtgggcgatg ttcttcacat ctaggagagc ctggtggtgg
     1141 acataggcct gacagtagta acaccaggct gacaggtcga tgtagctgag gaccagcggg
     1201 tgtccagaat ttccatggtg ttggagcatg tggccattga tgtaacgacc acagtagacc
     1261 tgatagcaag agagacacac ccaattctct tggattgttc cacagtcccc acaaggttgg
     1321 gtcacgtcta ggcctgctgc aggtatgggg catactgcca ccaaatgggg acaccagggc
     1381 agtggtgtca cagcataaaa tatggcctga tcagtgaggc ccctagatcc ctgcatcagc
     1441 atcgaatcag ccatgtcctg acctccagct gcctctccta gtaggttctc ctctcctggg
     1501 gcctgagatt ctgaggcccc ctgagattcg ctgcctagct ccaaggtcct gagactccca
     1561 atcagtgtac tgggagatat ctggggtgta gttccctgca caggtgaggt tgggggggtc
     1621 tggtggtctg tgctcgaggc tagaggagtt tggatcagct cggtgccccc tggagcctcc
     1681 tctgacgtag tctggtccag tgtggctccc tccatggctg cctccgagat ggtctgggcc
     1741 agtgtagctc ctcctacagc ctcctctgag gtagtctggc ccagcgtggc tcccccaaca
     1801 gcatcctctg aggtggtctg gtccagaatg gctcctccca cagtctcctc tgaggtggtc
     1861 tggtccggag tggctccccc gacagcctcc tctgaggtgg tctggcccag catggctccc
     1921 ccaatggctg cctcagaaat ggtctgggcc agagtggctc cccctgtggc tgcctctgag
     1981 ggctggtcct gagtgagggc caccacagct gtctctgagt tagtctggcc tggagtggac
     2041 tcttccccaa atgatgccga ggtgactttc cccatgcctg cttccagaac cttcttttct
     2101 cgtgtggtca tccgctcagc taacctaggt ttggctggtt ggggtgcctt cttggtgacc
     2161 aacttagaac tggagggtcc ttctctgtct tctaccttca tgacccgtaa gctgcgccag
     2221 tatctgcgat ggacttggat ggtctcagtg attgaggcca gggcccctga tagtgggggc
     2281 cgtggcaggg tcagcagggg tggtgggtct ccaaggaggg agcgagtgca ggcagccatg
     2341 gactctgaga tggatgtcag gttatagcca ccctctagga taaggataat gcggccactg
     2401 gcaaggccca tcagcaggtg ggtgaggtgg gcataaccct caggtgacac ctggcagccc
     2461 cccagcggat ccccccgtgc agcatcaaag ccagctgaga ccagcaccag ttctgggtta
     2521 aactcgtagg caatgggaag caccaggcga tgccaggcag ctaggtagtc agcatcaccc
     2581 atgcggggcc cgttccatgc cacgttgacg gtgaagcctg tgcccgcagc ccggccgatc
     2641 tggctgctgg caccctcatc ccccatgggg aagaaggtgc catgatcata gcggtgcagg
     2701 gacacatata gcacactggg gtcatcctca aacatgtgct gagttccatt accgtggtgg
     2761 acatcccaat ccacaatcag gatccgtagg gcatgcccac tgatagtctg ggcatggcga
     2821 gcagccacag ccacagagtt gaaaaagcaa aaaccgcaag ctgcatcctg ctctgcgtgg
     2881 tgtcctgggg gacgcaccac agcagcacca ttcagaacct ctcctgagag cacagcctcc
     2941 accaggcggc aggcagcgcc agtggcaagc tgtgcacagg cgaaggtact ggggcagata
     3001 tagatggagt caaagttgga actctcacgg tgcagctccc gggttttcat tttctctgtg
     3061 gcccggagat gacccacgta ctcagcactg tgacaggtga gcagctcagc ctctgtggca
     3121 gggcgcggtg tcagggtgag gcagcgcccg gcaaggccca gctcctccag acggcacatg
     3181 atccgcaaga tgcgctgggg tacctcaggg tggtggctgt cccacaagtt gcagtgattc
     3241 atcatatttt ggtcatagac cagccctgtg cgagactgta gcactggcca tgtcaggatt
     3301 gggagcacag ggggctccca gggtccttcc tcctcgctct cctctacatt gtcctcctcc
     3361 atgttgtccc tctccacggt ctcagttgat ctcacaagaa cctcccagaa gggctcaagg
     3421 gcttccagag cacaggaaac tgaagcctgg gcactccggc agggggcacc aggtgactcc
     3481 agcatggggc aagggtctcc cagaagggtg tggagcgaag cactgacgcc ttcagccagg
     3541 gcgcggaggt tgtagccacc ctccagagac aggatcagct tgcctcctgc cagacccatg
     3601 agcaggtggg ttagctgggc gaaccctgcc ggagtggcgg ccatctcacc cttggggtcc
     3661 ccttgcaggg catcaaatcc agcagccacc aggaccagct gaggctggaa ctcgagggcg
     3721 actggcagca ggacgtgcag gaaagcagca atgtagtcag catcccgcat ccccacctgg
     3781 ttccaaggca cattgatggt atatccttgg ccttggccga aacctgtggt ggaccagtta
     3841 gaggccttca ggtggggcca gaacctaccc tgctcgtagc ggtggatgga gaaatagagg
     3901 acactggggt cctggtcgaa ggtgaactgt gttccttgac cgtggtgcac atcccaatct
     3961 acgataagga ccctccggat gcggtgtttc tgttgagcat agcgggctgc cacagccacg
     4021 tggttgaaca tgcaatagcc atccataaga ctgtgctggg cgtgatgtcc aggaggccta
     4081 atgatggcca tgccattccg gatctcagcc cccaggaccg catccaccag cctgaggaca
     4141 gagcctgagg ccaggcaggc acaggagtat gagttcggat gcagataaac tgagtcgtag
     4201 gtgtctgcta ggacacggag ttctccctca ttcatgtact gggttgtttc catcagatca
     4261 atatattcta ggctgtgaac caacatcagc tcttcctttt cagcaaaccg ggcctgaaag
     4321 gacacgcagc gatctaggag gccctcctgg atcagttgct ccttgatggc atggagccgc
     4381 tcagggcctt ccgggaagct gtcatcccag aggcaatgga attcatttaa ctgctcatcc
     4441 aacaccaagc cagtgccagc cagtgcttca gcctcaaggt tcagatccat cccttgcagt
     4501 cccacgatta ggtcttcttc cattgcttgg ccgagcttct tcattttgcc tttcttcttt
     4561 acctccgcta gattggggat agagcgggga acggctccct ttttaatatt tcgcttcgaa
     4621 gtgacactgg agtcctgagg gggcgactgg gggttctgcc tacttcttcg ctgcctggtt
     4681 gtggtggaat cctggccggt tgaggtcatg gcggccgcca gtgtgatgga tatctgcaga
     4741 attccagcac actggcggcc gttactagtg gatccgcccg ggccaagctt ggggtcgaca
     4801 gcacaaacca gcacgttgcc caggagctgt aggaaaaaga agaaggcatg aacatggtta
     4861 gcagaggggc ccggtttgga ctcagagtat tttatcctca tctcaaacag tgtatatcat
     4921 tgtaaccata aagagaaagg caggatgatg accaggatgt agttgtttct accaataaga
     4981 atatttccac gccagccaga atttatatgc agaaatattc taccttatca tttaattata
     5041 acaattgttc tctaaaactg tgctgaagta caatataata taccctgatt gccttgaaaa
     5101 aaaagtgatt agagaaagta cttacaatct gacaaataaa caaaagtgaa tttaaaaatt
     5161 cgttacaaat gcaagctaaa gtttaacgaa aaagttacag aaaatgaaaa gaaaataaga
     5221 ggagacaatg gttgtcaaca gagtagaaag tgaaagaaac aaaattatca tgagggtcca
     5281 tggtgataca agggacatct tcccattcta aacaacaccc tgaaaacttt gccccctcca
     5341 tataacatga attttacaat agcgaaaaag aaagaacaat caagggtccc caaactcacc
     5401 ctgaagttct caggatcgac tctagcttgg gtctccctat agtgagtcgt attaatttcg
     5461 ataagccagt aagcagtggg ttctctagtt agccagagag ctctgcttat atagacctcc
     5521 caccgtacac gcctaccgcc catttgcgtc aatggggcgg agttgttacg acattttgga
     5581 aagtcccgtt gattttggtg ccaaaacaaa ctcccattga cgtcaatggg gtggagactt
     5641 ggaaatcccc gtgagtcaaa ccgctatcca cgcccattga tgtactgcca aaaccgcatc
     5701 accatggtaa tagcgatgac taatacgtag atgtactgcc aagtaggaaa gtcccataag
     5761 gtcatgtact gggcataatg ccaggcgggc catttaccgt cattgacgtc aatagggggc
     5821 gtacttggca tatgatacac ttgatgtact gccaagtggg cagtttaccg taaatactcc
     5881 acccattgac gtcaatggaa agtccctatt ggcgttacta tgggaacata cgtcattatt
     5941 gacgtcaatg ggcgggggtc gttgggcggt cagccaggcg ggccatttac cgtaagttat
     6001 gtaacgcgga actccatata tgggctatga actaatgacc ccgtaattga ttactattaa
     6061 taactagtca ataatcaatg tcaacgcgta tatctggccc gtacatcgcg aagcagcgca
     6121 aaacgcctaa ccctaagcag attcttcatg caattgtcgg tcaagccttg ccttgttgta
     6181 gcttaaattt tgctcgcgca ctactcagcg acctccaaca cacaagcagg gagcagatac
     6241 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatag gggatcggga
     6301 gatctcccga tccgtcgacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     6361 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     6421 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     6481 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     6541 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     6601 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     6661 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     6721 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     6781 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     6841 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     6901 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     6961 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     7021 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     7081 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     7141 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     7201 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     7261 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     7321 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     7381 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     7441 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     7501 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     7561 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     7621 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     7681 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     7741 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     7801 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     7861 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     7921 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     7981 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     8041 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     8101 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     8161 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     8221 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     8281 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     8341 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     8401 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
     8461 gaaacagcta tgaccatgat tacgccaagc tctagctaga ggtcgacggt atacagacat
     8521 gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt
     8581 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca
     8641 agttggggtg ggcgaagaac tccagcatga gatccccgcg ctggaggatc atccagccgg
     8701 cgtcccggaa aacgattccg aagcccaacc tttcatagaa ggcggcggtg gaatcgaaat
     8761 ctcgtgatgg caggttgggc gtcgcttggt cggtcatttc gaaccccaga gtcccgctca
     8821 gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag cggcgatacc
     8881 gtaaagcacg aggaagcggt cagcccattc gccgccaagc tcttcagcaa tatcacgggt
     8941 agccaacgct atgtcctgat agcggtccgc cacacccagc cggccacagt cgatgaatcc
     9001 agaaaagcgg ccattttcca ccatgatatt cggcaagcag gcatcgccat gggtcacgac
     9061 gagatcctcg ccgtcgggca tgcgcgcctt gagcctggcg aacagttcgg ctggcgcgag
     9121 cccctgatgc tcttcgtcca gatcatcctg atcgacaaga ccggcttcca tccgagtacg
     9181 tgctcgctcg atgcgatgtt tcgcttggtg gtcgaatggg caggtagccg gatcaagcgt
     9241 atgcagccgc cgcattgcat cagccatgat ggatactttc tcggcaggag caaggtgaga
     9301 tgacaggaga tcctgccccg gcacttcgcc caatagcagc cagtcccttc ccgcttcagt
     9361 gacaacgtcg agcacagctg cgcaaggaac gcccgtcgtg gccagccacg atagccgcgc
     9421 tgcctcgtcc tgcagttcat tcagggcacc ggacaggtcg gtcttgacaa aaagaaccgg
     9481 gcgcccctgc gctgacagcc ggaacacggc ggcatcagag cagccgattg tctgttgtgc
     9541 ccagtcatag ccgaatagcc tctccaccca agcggccgga gaacctgcgt gcaatccatc
     9601 ttgttcaatc atgcgaaacg atcctcatcc tgtctcttga tcagatccga aaatggatat
     9661 acaagctccc gggagctttt tgcaaaagcc taggcctcca aaaaagcctc ctcactactt
     9721 ctggaatagc tcagaggcag aggcg
//
