LOCUS       sequence-35082-e         660 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Levy C, Khaled M, Iliopoulos D, Janas MM, Schubert S, Pinner S, Chen
            PH, Li S, Fletcher AL, Yokoyama S, Scott KL, Garraway LA, Song JS, 
            Granter SR, Turley SJ, Fisher DE, Novina CD
  TITLE     Intronic miR-211 assumes the tumor suppressive function of its host 
            gene in melanoma.
  JOURNAL   Mol Cell. 2010 Dec 10. 40(5):841-9.
  PUBMED    21109473
REFERENCE   2  (bases 1 to 660)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2010 Dec 10. 40(5):841-9."
COMMENT     Sequence Label: PLKO-mcherry-luc-puro-shSCR
FEATURES             Location/Qualifiers
     source          1..660
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     123..142
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    297..414
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ctgttagaga gataattgga attaatttga ctgtaaacac aaagatatta gtacaaaata
       61 cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa
      121 tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct ttatatatct
      181 tgtggaaagg acgaaacacc ggaattctcc gaacgtgtca cgtctcgaga cgtgacacgt
      241 tcggagaatt tttttgaatt ctcgacctcg agacaaatgg cagtattcat ccacaatttt
      301 aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca
      361 acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt
      421 tattacaggg acagcagaga tccactttgg ccgcggctcg agggggttgg ggttgcgcct
      481 tttccaaggc agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa
      541 acgcagcggc gccgaccctg ggtctcgcac attcttcacg tccgttcgca gcgtcacccg
      601 gatcttcgcc gctacccttg tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg
//
