LOCUS       sequence-350820-        5906 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of Lamp1 fused to RFP. Used for localization to
            the lysosome..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5906)
  AUTHORS   Sherer NM, Lehmann MJ, Jimenez-Soto LF, Ingmundson A, Horner SM, 
            Cicchetti G, Allen PG, Pypaert M, Cunningham JM, Mothes W
  TITLE     Visualization of retroviral replication in living cells reveals 
            budding into multivesicular bodies.
  JOURNAL   Traffic 2003 Nov;4(11):785-801.
  PUBMED    14617360
REFERENCE   2  (bases 1 to 5906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Traffic"; 
            date: "2003-11"; volume: "4(11)"; pages: "785-801"
COMMENT     Sequence Label: Lamp1-RFP
FEATURES             Location/Qualifiers
     source          1..5906
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     148..168
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        complement(193..297)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      324..779
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(411..430)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     621..642
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    786..907
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(797..816)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     851..870
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(1042..1716)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             complement(1042..1716)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(1101..1124)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(1299..1318)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1568..1586
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      1713..1722
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(join(1738..2895,2896..2958))
                     /codon_start=1
                     /product="rat lysosome-associated membrane glycoprotein 1"
                     /label=LAMP1
                     /translation="MAAPGARRPLLLLLLAGLAHSAPALFEVKDNNGTACIMASFSASF
                     LTTYEAGHVSKVSNMTLPASAEVLKNSSSCGEKNASEPTLAITFGEGYLLKLTFTKNTT
                     RYSVQHMYFTYNLSDTQFFPNASSKGPDTVDSTTDIKADINKTYRCVSDIRVYMKNVTI
                     VLWDATIQAYLPSSNFSKEETRCPQDQPSPTTGPPSPSPPLVPTNPSVSKYNVTGDNGT
                     CLLASMALQLNITYMKKDNTTVTRAFNINPSDKYSGTCGAQLVTLKVGNKSRVLELQFG
                     MNATSSLFFLQGVQLNMTLPDAIEPTFSTSNYSLKALQASVGNSYKCNSEEHIFVSKAL
                     ALNVFSVQVQAFRVESDRFGSVEECVQDGNNMLIPIAVGGALAGLVLIVLIAYLIGRKR
                     SHAGYQTI"
     regulatory      2951..2960
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      2955..2964
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(3033..3236)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(3062..3082)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        3237..3540
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(3715..4303)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3795..3814)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    4632..4679
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     4704..4723
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(4911..5705)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(4911..5705)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5022..5041)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     5632..5651
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(join(5740..5906,1..191))
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5754..5889
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        join(5906,1..191)
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
ORIGIN
        1 ggactatggt tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc
       61 ctggggactt tccacacctg gttgctgact aattgagatg catgctttgc atacttctgc
      121 ctgctgggga gcctggggac tttccacacc ctaactgaca cacattccac agctggttct
      181 ttccgcctca ggactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
      241 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
      301 atttccccga aaagtgccac ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
      361 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
      421 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
      481 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
      541 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
      601 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
      661 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
      721 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta
      781 cgccttaaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
      841 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
      901 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga
      961 ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg atctagagtc
     1021 gcggccgctt tacttgtaca aggcgccggt ggagtggcgg ccctcggcgc gctcgtactg
     1081 ttccacgatg gtgtagtcct cgttgtggga ggtgatgtcc agcttgatgt cggtcttgta
     1141 ggcgccgggc agctgcacgg gcttcttggc catgtaggtg gtcttgacct cggcgtcgta
     1201 gtggccgccg tccttcagct tcagcctcat cttgatctcg cccttcaggg cgccgtcctc
     1261 ggggtacatc cgctcggtgg aggcctccca gcccatggtc ttcttctgca ttacggggcc
     1321 gtcggagggg aagttggtgc cgcgcagctt caccttgtag atgaactcgc cgtcctgcag
     1381 ggaggagtcc tgggtcacgg tcaccacgcc gccgtcctcg aagttcatca cgcgctccca
     1441 cttgaagccc tcggggaagg acagcttcaa gtagtcgggg atgtcggcgg ggtgcttcac
     1501 gtaggccttg gagccgtact ggaactgagg ggacaggatg tcccaggcga agggcagggg
     1561 gccgcccttg gtcaccttca gcttggcggt ctgggtgccc tcgtaggggc ggccctcgcc
     1621 ctcgccctcg atctcgaact cgtggccgtt cacggagccc tccatgcgca ccttgaagcg
     1681 catgaactcc ttgatgacgt cctcggagga ggccatggtg gcgaccggtg gatcccggat
     1741 ggtctgatag cccgcgtgac tcctcttcct gccgatgagg taggcgatga ggacgatgag
     1801 gaccagccct gccagggccc cgcccacagc aatggggatc agcatgttgt taccgtcctg
     1861 tacacactct tccacagacc caaacctgtc actttctacc ctgaaagcct ggacttgcac
     1921 gctgaagaca ttgagggcga gcgccttgct gacaaagatg tgctcctcac tgttgcactt
     1981 gtatgagttg ccgacactgg cctgaagagc tttcagggaa tagttggagg tgctgaacgt
     2041 gggctctatg gcatcaggaa gagtcatgtt caactgaact ccttgcagga aaaacaggct
     2101 agaagtggca ttcatcccaa actgcagctc caggactctg ctcttgttcc ccaccttcag
     2161 ggtcaccaac tgggcaccgc aagtcccact atatttgtca cttgggttga tgttgaatgc
     2221 tctggtcaca gtcgtgttgt ccttcttcat gtaggtgatg ttgagttgca gtgccataga
     2281 ggccagcagg caggttccat tgtcaccagt cacattgtac ttggacacac tggggtttgt
     2341 gggcacgagt ggtggtgagg ggctgggtgg cccagtagtt ggggaaggtt gatcctgtgg
     2401 gcagcgtgtc tcttccttgc tgaagttgct actcggcagg taggcctgga tagtagcgtc
     2461 ccagagcaca atggtcacat tcttcatgta gaccctgatg tcgctgacac atcggtatgt
     2521 tttgttgatg tctgccttga tgtcagttgt ggaatccaca gtgtcgggcc ctttggagct
     2581 ggcattggga aagaattgtg tgtctgacag gttatatgtg aaatacatgt gctggacact
     2641 gtaacgtgtt gtgttttttg tgaaggtgag tttcagtaaa tatccttctc caaaggtgat
     2701 tgcgagggtg ggctcagaag cattcttttc accacaagag ctgctattct tcaggacttc
     2761 tgcagaggct ggcagggtca tattcgagac cttagaaaca tgtccagcct cataggtggt
     2821 cagaaaggag gcagagaagc tggccattat acacgctgtg ccgttgttgt ctttcacctc
     2881 gaacagtgct ggggcgctgt gtgcaaggcc tgccagcagc aacaggagca gcggccgccg
     2941 ggcgcccggg gccgccatgg tggcgagaat tcgaagcttg agctcgagat ctgagtccgg
     3001 tagcgctagc ggatctgacg gttcactaaa ccagctctgc ttatatagac ctcccaccgt
     3061 acacgcctac cgcccatttg cgtcaatggg gcggagttgt tacgacattt tggaaagtcc
     3121 cgttgatttt ggtgccaaaa caaactccca ttgacgtcaa tggggtggag acttggaaat
     3181 ccccgtgagt caaaccgcta tccacgccca ttgatgtact gccaaaaccg catcaccatg
     3241 gtaatagcga tgactaatac gtagatgtac tgccaagtag gaaagtccca taaggtcatg
     3301 tactgggcat aatgccaggc gggccattta ccgtcattga cgtcaatagg gggcgtactt
     3361 ggcatatgat acacttgatg tactgccaag tgggcagttt accgtaaata ctccacccat
     3421 tgacgtcaat ggaaagtccc tattggcgtt actatgggaa catacgtcat tattgacgtc
     3481 aatgggcggg ggtcgttggg cggtcagcca ggcgggccat ttaccgtaag ttatgtaacg
     3541 cggaactcca tatatgggct atgaactaat gaccccgtaa ttgattacta ttaataacta
     3601 atgcatggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     3661 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     3721 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     3781 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     3841 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     3901 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     3961 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     4021 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     4081 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     4141 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     4201 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     4261 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     4321 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     4381 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     4441 ctaaagtata tatgagtaac ctgaggctat ggcagggcct gccgccccga cgttggctgc
     4501 gagccctggg ccttcacccg aacttggggg gtggggtggg gaaaaggaag aaacgcgggc
     4561 gtattggccc caatggggtc tcggtggggt atcgacagag tgccagccct gggaccgaac
     4621 cccgcgttta tgaacaaacg acccaacacc gtgcgtttta ttctgtcttt ttattgccgt
     4681 catagcgcgg gttccttccg gtattgtctc cttccgtgtt tcagttagcc tccccctagg
     4741 gtgggcgaag aactccagca tgagatcccc gcgctggagg atcatccagc cggcgtcccg
     4801 gaaaacgatt ccgaagccca acctttcata gaaggcggcg gtggaatcga aatctcgtga
     4861 tggcaggttg ggcgtcgctt ggtcggtcat ttcgaacccc agagtcccgc tcagaagaac
     4921 tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc
     4981 acgaggaagc ggtcagccca ttcgccgcca agctcttcag caatatcacg ggtagccaac
     5041 gctatgtcct gatagcggtc cgccacaccc agccggccac agtcgatgaa tccagaaaag
     5101 cggccatttt ccaccatgat attcggcaag caggcatcgc catgggtcac gacgagatcc
     5161 tcgccgtcgg gcatgctcgc cttgagcctg gcgaacagtt cggctggcgc gagcccctga
     5221 tgctcttcgt ccagatcatc ctgatcgaca agaccggctt ccatccgagt acgtgctcgc
     5281 tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc
     5341 cgccgcattg catcagccat gatggatact ttctcggcag gagcaaggtg agatgacagg
     5401 agatcctgcc ccggcacttc gcccaatagc agccagtccc ttcccgcttc agtgacaacg
     5461 tcgagcacag ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg cgctgcctcg
     5521 tcttgcagtt cattcagggc accggacagg tcggtcttga caaaaagaac cgggcgcccc
     5581 tgcgctgaca gccggaacac ggcggcatca gagcagccga ttgtctgttg tgcccagtca
     5641 tagccgaata gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca
     5701 atcatgcgaa acgatcctca tcctgtctct tgatcgatct ttgcaaaagc ctaggcctcc
     5761 aaaaaagcct cctcactact tctggaatag ctcagaggcc gaggcggcct cggcctctgc
     5821 ataaataaaa aaaattagtc agccatgggg cggagaatgg gcggaactgg gcggagttag
     5881 gggcgggatg ggcggagtta ggggcg
//
