LOCUS       sequence-350830-       12212 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Stabilized lacZ expression vector containing alp7A and hok/sok.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12212)
  AUTHORS   Danino T, Prindle A, Kwong GA, Skalak M, Li H, Allen K, Hasty J, 
            Bhatia SN
  TITLE     Programmable probiotics for detection of cancer in urine.
  JOURNAL   Sci Transl Med. 2015 May 27;7(289):289ra84. doi: 
            10.1126/scitranslmed.aaa3519.
  PUBMED    26019220
REFERENCE   2  (bases 1 to 12212)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Transl 
            Med."; date: "2015-05-27"; volume: "7(289):289ra84. doi"; pages: " 
            10.1126/scitranslmed.aaa3519"
COMMENT     Sequence Label: pTKW106alp7A
FEATURES             Location/Qualifiers
     source          1..12212
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(join(1..7,8..23,24..29))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     primer_bind     complement(300..317)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(471..1059)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(551..570)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(1982..2001)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1993..2808
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             1993..2808
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2066..2085)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     3530..3549
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3768..3872)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             complement(3985..5067)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     5029..5048
                     /label=LacI-R
                     /note="LacI, reverse primer"
     terminator      complement(8651..8737)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     8890..8907
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     CDS             complement(9080..12127)
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
                     LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
                     FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
                     DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
                     FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
                     TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
                     LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
                     YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
                     NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
                     LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
                     WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
                     FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
                     PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
                     SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
                     ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
                     ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
                     CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
                     SWSPSVSAELQLSAGRYHYQLVWCQK"
     primer_bind     12103..12125
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(12178..12200)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12189..12205
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 cattatacga gccgatgatt aattgtcaac agctcatttc agaatatttg ccagaaccgt
       61 tatgatgtcg gcgcaaaaaa cattatccag aacgggagtg cgccttgagc gacacgaatt
      121 atgcagtgat ttacgacctg cacagccata ccacagcttc cgatggctgc ctgacgccag
      181 aagcattggt gcaccgtgca gtcgatgata agctgtcaaa catgagaatt aattctgcat
      241 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
      301 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
      361 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
      421 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
      481 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
      541 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
      601 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
      661 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
      721 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
      781 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
      841 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
      901 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
      961 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     1021 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     1081 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     1141 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     1201 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     1261 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     1321 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     1381 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     1441 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     1501 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     1561 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     1621 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     1681 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     1741 actgtcatgc catccgtaag atgcttttct gtgactggtg agtgactcta gaggatcccc
     1801 agatcacgca tcttcccgac aacgcagacc gttccgtggc aaagcaaaag ttcaaaatca
     1861 ccaactggtc cggggggggg ggaaagccac gttgtgtctc aaaatctctg atgttacatt
     1921 gcacaagata aaaatatatc atcatgaaca ataaaactgt ctgcttacat aaacagtaat
     1981 acaaggggtg ttatgagcca tattcaacgg gaaacgtctt gctcgaggcc gcgattaaat
     2041 tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca
     2101 ggtgcgacaa tctatcgatt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat
     2161 ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg
     2221 gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta
     2281 ctcaccactg cgatccccgg gaaaacagca ttccaggtat tagaagaata tcctgattca
     2341 ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt
     2401 tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg
     2461 aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa
     2521 caagtctgga aagaaatgca taagcttttg ccattctcac cggattcagt cgtcactcat
     2581 ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat
     2641 gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc
     2701 ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct
     2761 gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc agaattggtt
     2821 aattggttgt aacactggcg ggtaccgagc tcgaattccc acaacatcag caaggagaaa
     2881 ggggctaccg gcgaaccagc agccccttta taaaggcgct tcagtagtca gaccagcatc
     2941 agtcctgaaa aggcgggcct gcgcccgcct ccaggttgct acttaccgga ttcgtaagcc
     3001 atgaaagccg ccacctccct gtgtccgtct ctgtaacgaa tctcgcacag cgattttcgt
     3061 gtcagataag tgaatatcaa cagtgtgaga cacacgatca acacacacca gacaagggaa
     3121 cttcgtggta gtttcatggc cttcttctcc ttgcgcaaag cgcggtaaga ggctatcctg
     3181 atgtggacta gacataggga tgcctcgtgg tggttaatga aaattaactt actacggggc
     3241 tatcttcttt ctgccacaca acacggcaac aaaccacctt cacgtcatga ggcagaaagc
     3301 ctcaagcgcc gggcacatca tagcccatat acctgcacgc tgaccacact cactttccct
     3361 gaaaataatc cgctcattca gaccgttcac gggaaatccg tgtgattgtt gccgcatcac
     3421 gctgcctccc ggagtttgtt cggccggatc ctctagagtc actcaaccaa gtcattctga
     3481 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     3541 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     3601 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     3661 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     3721 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     3781 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     3841 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     3901 gtgaggttct tgcaggtcgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     3961 taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     4021 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag
     4081 ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc
     4141 ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt
     4201 gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac
     4261 cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc
     4321 catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat
     4381 ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat
     4441 ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact
     4501 taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc
     4561 cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac
     4621 atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc
     4681 atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc
     4741 cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag
     4801 ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact
     4861 ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt
     4921 gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac
     4981 gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc
     5041 gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct cttccgggcg
     5101 ctatcatgcc ataccgcgaa aggttttgca ccacctaggt cattagcctc caatcttata
     5161 gtgaaactcc gcaaacttcg tttcctcaat attgggaata ctcgataact tttgttcagc
     5221 tttttcataa aaagaaattg ctttaacata atttttttta aaaaactcat aatcccctgc
     5281 aaacaattca aaataaaaat ttattaaaag atcatgactt cccttactta acatattaac
     5341 gacatcaccc atagatgcct cttgatcaaa acaaaggcta tgacgatgat ccagtagaat
     5401 aaaataatca accaggtcct tattatcttc agcctgactt aacaattgct ttatttcagt
     5461 tcttttctct atagactcat gaaccttctc ctgcataata tagtcatgcc atttattcaa
     5521 cagattgcct acatagggag acggtacttt ttttactttg gacaacatac acttccctcc
     5581 agctattcaa aaacaaaact agtcctaata cttatcggca tcatcaaaca ttttaaaaca
     5641 cagaacattt agtacatagt gcttaatgtt caaatctcat tttgatgctc tgaacccaag
     5701 catgtactga tatcatacta aacggctcaa acacaatcta tacaagtttt taagataggc
     5761 caaagggaat aacagtatac gttagtgaaa tcccacagga aaaatatatt aaaatactaa
     5821 tgttctatca aacgaacaac ccttaaaaag gaacttaaaa cctctgggtt ttaaggaaat
     5881 tcgcatttat ttagtgtttt tctcttgact ttgagaactt gaaactagca gaatagctga
     5941 ctgttctagg aaacagggcg aatttcgatt gcctatgtct gtcgcgcaaa aaataaaaac
     6001 ggacagacat aggcaatcga tcaggatttg aaactagcgt catagagacg tctgaggttt
     6061 ccagctctgc cttgctatcg ccaggctttc gcctgccatg acctttttac atacaatgct
     6121 tgtcctgtat gcaacttcta tggggtttgt ctcgtgttct ctcacacggt cacactcaat
     6181 tgtgtgccgc tgcatagaag cttggccata gttgcccgca ccgtagtgcg ccaagcaacc
     6241 tagtggttta tccacattct ccggaccgtt aatggccgtc ctcgccattc accacaagcg
     6301 cagcaaggaa cgcttattgt ggtatatccc cgggtttgcg gtggacgggg caactcctga
     6361 cgtcagttta ttttacaccc cttaacggca gctgggtgac aaacaaaaaa cgacagaaaa
     6421 ccacggtttg ataccctcca aacagtggtt ttctgtcgtc caaaaatagc cgaaaagtgt
     6481 tgacgtatac acttgttttc ggtaaaatga agacataact taaacattgt aagtgagggc
     6541 ttacaaacca agtgttcgat gctgcaacat cggacacttt ttatttgtca ttctttattt
     6601 gtattcaatt ttgcaaatag ctcgcaaaca aaatatgtat catcaaatct attaaccttg
     6661 ttgtctgcaa acaacagggt tttttgttgt ttatttagaa taactagaac cagaattcaa
     6721 tgccaaaact ttcacattga cttaacttga ctttatctta cacgattttt tttttgacgt
     6781 aaagccccgg gcctgaaatc acttttctct actgatttca ctgatttcat ttttattata
     6841 taatcctcaa atagcctgta ttcactgatt ttaaatgtga tttcatttta ttgactttag
     6901 tgatataaga tgctagtatt gaggaaagtg aaatcaaagg agagaataaa aatatgaata
     6961 tttctcgtat gaacgtggac tttggaaaca gtatgtacat gaatttaatt gatggttatt
     7021 tttttgaatt gcctacaaat gtagtagaga tatctaaaga agctgctgaa ggaaaattta
     7081 cgagtatcgt tgaagatccg gcagatttaa aggaccggtt attagtttct acagttattg
     7141 atgaaacaga gagatatttt ctagttggtg aacttgctga accagaagtg ttaggcaacc
     7201 aacacatcaa gaagttacat aataaagtag agtcacatat tccatacgta acatttttag
     7261 ctgcaactgc ttattaccaa gcgctaaaag gcaaacgtga agataatgaa gttactattg
     7321 aatactttca aacaatgcta ccaatttggc ttcttaaaaa attggataag ttcagtgaaa
     7381 tgcagaaaag gatggcatct aaatttttgg gcactcacca agtaaaggtg ctgacattag
     7441 gattagaaaa agagcttact ataaaagtgg aagatgcagc gtgcaggatc gaatctgaag
     7501 tagcaagatg ggcaataaag aaaaactttg acctagaaga taaagactat gccgaacaat
     7561 ttaaaaatta tgacgtagtt ttttgtgatt taggtggcgg aacagatgat ctagtattac
     7621 taccagctgg attaaaaccg ccaaaaagtc gtgattcttt tgtttctaat accgaagcac
     7681 cgtttttagc gcacttagaa aaattgagaa aagaaaaact cctagagcac tttgatagcg
     7741 ttagggagct tgaaaagttt atatactcaa atattggaaa aactaagatg gaacgaagag
     7801 acgggaatac cggtcagaaa tttgatttaa ctgatatcat caaaaaatct cttaaagaat
     7861 acacagaaat caaaatagcc caagctgaaa atacgttccc tgcaccaaaa gataaggttt
     7921 acaaatacct ttattttggc ggtgttggcg aggtgcttga agaatcaatt agtgtggtta
     7981 ctgaagagag atatggccgt gatatttctg aatcaaatca tatagttgct gaggatgcaa
     8041 gactgctcaa cttatatggc cttgaagttt taagccgcgc tgaacaagta aagaaacagg
     8101 caaatgaaaa agaggcacaa tcaatttagg tgattagaaa tggggaaaaa caaaagaatt
     8161 ccactcttta atgtccgaac aacacaaatg tctgatgaaa tgtacgattt tgttttagag
     8221 cagattagta cattcagtaa aggtaagagt aagggtacct ttagagagta tgcctttcag
     8281 ctcatagaaa gggacatgca acaacagaaa gaggaacagc agaatagaga aaaagatcgt
     8341 catgttcatg atgaattaat tgccatgaga gaagaaatga agaaagaatt tcgtgatttg
     8401 aggaagaaaa ttgatcaggg atcgatctac gtagaacaca aaacagctga tccaaagtca
     8461 gcttcagaaa cgattgaaga aggtcagtta atcactgaaa aaatcactgg aactattgaa
     8521 gaagaatacg actatgattt ttaagagcct ggattaatct aggctctttt tttatgccat
     8581 ttaagggagg attgcatgac aaactttttt tagttgcaac acagacgccc tgagcaacgc
     8641 taggcggcgg atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa
     8701 aggcccagtc tttcgactga gcctttcgtt ttatttgatg cctggcagtt ccctactctc
     8761 gcatggggag accccacact accatcggcg ctacggcgtt tcacttctga gttcggcatg
     8821 gggtcaggtg ggaccaccgc gctactgccg ccaggcaaat tctgttttat cagaccgctt
     8881 ctgcgttctg atttaatctg tatcaggctg aaaatcttct ctcatccgcc aaaacaggga
     8941 atttatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca
     9001 ttcgccattc aggctgcaaa tagtacataa tggatttcct tacgcgaaat acgggcagac
     9061 atggcctgcc cggttattat tatttttgac accagaccaa ctggtaatgg tagcgaccgg
     9121 cgctcagctg taattccgcc gatactgacg ggctccagga gtcgtcgcca ccaatcccca
     9181 tatggaaacc gtcgatattc agccatgtgc cttcttccgc gtgcagcaga tggcgatggc
     9241 tggtttccat cagttgctgt tgactgtagc ggctgatgtt gaactggaag tcgccgcgcc
     9301 actggtgtgg gccataattc aattcgcgcg tcccgcagcg cagaccgttt tcgctcggga
     9361 agacgtacgg ggtatacatg tctgacaatg gcagatccca gcggtcaaaa caggcggcag
     9421 taaggcggtc gggatagttt tcttgcggcc ctaatccgag ccagtttacc cgctctgcta
     9481 cctgcgccag ctggcagttc aggccaatcc gcgccggatg cggtgtatcg ctcgccactt
     9541 caacatcaac ggtaatcgcc atttgaccac taccatcaat ccggtaggtt ttccggctga
     9601 taaataaggt tttcccctga tgctgccacg cgtgagcggt cgtaatcagc accgcatcag
     9661 caagtgtatc tgccgtgcac tgcaacaacg ctgcttcggc ctggtaatgg cccgccgcct
     9721 tccagcgttc gacccaggcg ttagggtcaa tgcgggtcgc ttcacttacg ccaatgtcgt
     9781 tatccagcgg tgcacgggtg aactgatcgc gcagcggcgt cagcagttgt tttttatcgc
     9841 caatccacat ctgtgaaaga aagcctgact ggcggttaaa ttgccaacgc ttattaccca
     9901 gctcgatgca aaaatccatt tcgctggtgg tcagatgcgg gatggcgtgg gacgcggcgg
     9961 ggagcgtcac actgaggttt tccgccagac gccactgctg ccaggcgctg atgtgcccgg
    10021 cttctgacca tgcggtcgcg ttcggttgca ctacgcgtac tgtgagccag agttgcccgg
    10081 cgctctccgg ctgcggtagt tcaggcagtt caatcaactg tttaccttgt ggagcgacat
    10141 ccagaggcac ttcaccgctt gccagcggct taccatccag cgccaccatc cagtgcagga
    10201 gctcgttatc gctatgacgg aacaggtatt cgctggtcac ttcgatggtt tgcccggata
    10261 aacggaactg gaaaaactgc tgctggtgtt ttgcttccgt cagcgctgga tgcggcgtgc
    10321 ggtcggcaaa gaccagaccg ttcatacaga actggcgatc gttcggcgta tcgccaaaat
    10381 caccgccgta agccgaccac gggttgccgt tttcatcata tttaatcagc gactgatcca
    10441 cccagtccca gacgaagccg ccctgtaaac ggggatactg acgaaacgcc tgccagtatt
    10501 tagcgaaacc gccaagactg ttacccatcg cgtgggcgta ttcgcaaagg atcagcgggc
    10561 gcgtctctcc aggtagcgaa agccattttt tgatggacca tttcggcaca gccgggaagg
    10621 gctggtcttc atccacgcgc gcgtacatcg ggcaaataat atcggtggcc gtggtgtcgg
    10681 ctccgccgcc ttcatactgc accgggcggg aaggatcgac agatttgatc cagcgataca
    10741 gcgcgtcgtg attagcgccg tggcctgatt cattccccag cgaccagatg atcacactcg
    10801 ggtgattacg atcgcgctgc accattcgcg ttacgcgttc gctcatcgcc ggtagccagc
    10861 gcggatcatc ggtcagacga ttcattggca ccatgccgtg ggtttcaata ttggcttcat
    10921 ccaccacata caggccgtag cggtcgcaca gcgtgtacca cagcggatgg ttcggataat
    10981 gcgaacagcg cacggcgtta aagttgttct gcttcatcag caggatatcc tgcaccatcg
    11041 tctgctcatc catgacctga ccatgcagag gatgatgctc gtgacggtta acgcctcgaa
    11101 tcagcaacgg cttgccgttc agcagcagca gaccattttc aatccgcacc tcgcggaaac
    11161 cgacatcgca ggcttctgct tcaatcagcg tgccgtcggc ggtgtgcagt tcaaccaccg
    11221 cacgatagag attcgggatt tcggcgctcc acagtttcgg gttttcgacg ttcagacgta
    11281 gtgtgacgcg atcggcataa ccaccacgct catcgataat ttcaccgccg aaaggcgcgg
    11341 tgccgctggc gacctgcgtt tcaccctgcc ataaagaaac tgttacccgt aggtagtcac
    11401 gcaactcgcc gcacatctga acttcagcct ccagtacagc gcggctgaaa tcatcattaa
    11461 agcgagtggc aacatggaaa tcgctgattt gtgtagtcgg tttatgcagc aacgagacgt
    11521 cacggaaaat gccgctcatc cgccacatat cctgatcttc cagataactg ccgtcactcc
    11581 agcgcagcac catcaccgcg aggcggtttt ctccggcgcg taaaaatgcg ctcaggtcaa
    11641 attcagacgg caaacgactg tcctggccgt aaccgaccca gcgcccgttg caccacagat
    11701 gaaacgccga gttaacgcca tcaaaaataa ttcgcgtctg gccttcctgt agccagcttt
    11761 catcaacatt aaatgtgagc gagtaacaac ccgtcggatt ctccgtggga acaaacggcg
    11821 gattgaccgt aatgggatag gtcacgttgg tgtagatggg cgcatcgtaa ccgtgcatct
    11881 gccagtttga ggggacgacg acagtatcgg cctcaggaag atcgcactcc agccagcttt
    11941 ccggcaccgc ttctggtgcc ggaaaccagg caaagcgcca ttcgccattc aggctgcgca
    12001 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
    12061 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
    12121 aaacgacggg atccccgggt accgagctcg aattcgtaat caaattcatc gctgtttcct
    12181 gtgtgaaatt gttatccgct cacaattcca ca
//
