LOCUS       sequence-350831-       10850 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Spaghetti Monster (sm) 9xGCN4-Renilla mRNA reporter in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10850)
  AUTHORS   Gomez-Puerta S, Ferrero R, Hochstoeger T, Zubiri I, Chao J, Aragon 
            T, Voigt F
  TITLE     Live imaging of the co-translational recruitment of XBP1 mRNA to the
            ER and its processing by diffuse, non-polarized IRE1alpha.
  JOURNAL   Elife. 2022 Jun 22;11. pii: 75580. doi: 10.7554/eLife.75580.
  PUBMED    35730412
REFERENCE   2  (bases 1 to 10850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-06-22"; volume: "11. pii: 75580. doi"; pages: " 
            10.7554/eLife.75580"
COMMENT     Sequence Label: sm-9xGCN4-Renilla-MS2
FEATURES             Location/Qualifiers
     source          1..10850
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(12..28)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12..28)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(25..47)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    36..52
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(60..66,67..84,85..90))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    105..126
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(243..260)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(414..1002)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(494..513)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1173..1964,1965..2033))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1796..1815
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2034..2138)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2206..2224
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2262..2284)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2384..2403
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2597..2619
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2611..2628
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2612..2628
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3710..3757)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    3804..3822
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3840..3858
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3876..3894
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3912..3930
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3948..3966
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3984..4002
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4020..4038
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     4083..4107
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          4184..4316
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     4299..4318
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        4361..4379
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      4383..4392
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4458..4514
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4530..4586
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4602..4658
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5202..5258
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5274..5330
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5346..5402
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5628..5684
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5700..5756
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5772..5828
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5862..6791
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="ASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAENA
                     VIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAWF
                     ELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDIA
                     LIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPREI
                     PLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKVK
                     GLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     CDS             join(6792..6794,6795..7115)
                     /codon_start=1
                     /product="destabilization domain that can be stabilized by 
                     Shield1 in the ProteoTuner(TM) system"
                     /label=DD
                     /note="L106P mutant of FKBP12"
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKPE"
     CDS             6795..7118
                     /codon_start=1
                     /product="destabilization domain that can be stabilized by 
                     Shield1 in the ProteoTuner(TM) system"
                     /label=DD-C
                     /note="FKBP12 mutant optimized for use as a C-terminal tag"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KPE"
     polyA_signal    8648..8782
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     8726..8745
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     protein_bind    complement(8890..8923)
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     primer_bind     complement(8976..8992)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(9008..9030)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    9441..9798
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     LTR             9863..10332
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
ORIGIN
        1 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
       61 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
      121 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
      181 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
      241 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
      301 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
      361 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
      421 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
      481 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
      541 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
      601 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
      661 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
      721 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
      781 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
      841 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
      901 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
      961 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     1021 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt tgtcatgaga
     1081 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     1141 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     1201 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     1261 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     1321 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     1381 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     1441 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     1501 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     1561 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     1621 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     1681 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     1741 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     1801 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     1861 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     1921 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     1981 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     2041 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     2101 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     2161 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     2221 aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
     2281 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
     2341 gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt
     2401 gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
     2461 cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
     2521 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg
     2581 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga attaattcca
     2641 agctaagtga aagactctgt ctcaaaaacc aacctgacac ctgagccgga gcgttgcctc
     2701 tcactaccat aaatatatat acacaacaca cacacacaca cacacacaca cacacacaca
     2761 cacacacaca atcatacaca taaacacaat cacacacaca cacatgcaca atcacacaca
     2821 cacaatcaca cacaaacata catacacaca atcacacaca accatacaca catatacaca
     2881 atcacacaca caaacacaat cacacacaca cacatcaatc aatcaatcaa tcaatatacc
     2941 aaaaatgata gctcatacct gtaatcttag atgtgggaag gctaaaacag caggctaccc
     3001 atgacttcaa ggtcaccctg ggttacagag tagctaatag tctagcttga actatagtgt
     3061 aggatcttta aaaaaaaatt aaccaaccaa ccaacaaaca aacaaacaaa caacaacaac
     3121 aacaaatgga agacagagga caaaaaaaaa taaaagaaaa agtagaaagc caatggagat
     3181 actatcttaa aattagaaat atttatgaca cattctgaat actccatgtt tatttcaagg
     3241 aagccaatgt aactttaagt actatggcca tttaatgtaa atacttaaga aaaaaaacca
     3301 aattaatttt gatacatgct gcatgtgaaa gacccccgct gacgggtagt caatcactca
     3361 gaggagaccc tctcaaggaa cagcgagacc acaagtcgga tgcaactgca agagggttta
     3421 ttggatacac gggtacccgg gcgactcagt caatcggagg actggcgccc cgagtgaggg
     3481 gttgtgggct cgagctcggg gagcagaagc gcgcgaacag aagtcgagct agcttgccaa
     3541 acctacaggt ggggtctttc attcccccct ttttctggag actaaataaa atcttttatt
     3601 ttatctatgg ctcgtactct ataggcttca gctggtgata ttgttgagtc aaaactagag
     3661 cctggaccac tgatatcctg tctttaacaa attggactaa tccggatctg aagttcctat
     3721 actttctaga gaataggaac ttcggaatag gaacttcgcg gccagatctt ttgcggccgc
     3781 tttgggccgg ccgaattctt tactccctat cagtgataga gaacgtatga agagtttact
     3841 ccctatcagt gatagagaac gtatgcagac tttactccct atcagtgata gagaacgtat
     3901 aaggagttta ctccctatca gtgatagaga acgtatgacc agtttactcc ctatcagtga
     3961 tagagaacgt atctacagtt tactccctat cagtgataga gaacgtatat ccagtttact
     4021 ccctatcagt gatagagaac gtataagctt taggcgtgta cggtgggcgc ctataaaagc
     4081 agagctcgtt tagtgaaccg tcagatcgcc tggagcaatt ccacaacact tttgtcttat
     4141 accaactttc cgtaccactt cctaccctcg taaaaccggt caggtaagta tcaaggttac
     4201 aagacaggtt taaggagacc aatagaaact gggcttgtcg agacagagaa gactcttgcg
     4261 tttctgatag gcacctattg gtcttactga catccacttt gcctttctct ccacaggtgt
     4321 ccactcccag ttcaattaca gctcttaagg ctagagtact taatacgact cactataggc
     4381 tagccaccat gggcgccatc atacacgcgg ccgcaatcat cggtccgggt ggatctggag
     4441 gtggaggttc tggaggagaa gaacttttga gcaagaatta tcatcttgag aacgaagtgg
     4501 ctcgtcttaa gaaaggttct ggcagtggag aagaactgct ttcaaagaat taccacctgg
     4561 aaaatgaggt agctagactg aaaaagggga gcggaagtgg ggaggagttg ctgagcaaaa
     4621 attatcattt ggagaacgaa gtagcacgac taaagaaagg gtccggatcg ggtggcggcg
     4681 tgtcgaaagg cgaggagctg ttcacaggcg tggtccccat cctggttgag ctggacggtg
     4741 acgttaacgg ccacaaattt agcgtacgtg gtgagggcga gggtgacgct accaacggaa
     4801 agctgaccct gaaattcatc tgcacaactg gcaagctgcc ggtgccctgg cccacgctgg
     4861 taaccactct gggcggaggc gttcaatgtt tcagtcgtta cccggatcac atgaagcagc
     4921 atgacttctt caagtcggcc atgcccgaag gctacgtgca ggagcgcacc atcagtttta
     4981 aggatgacgg cacctacaag actcgcgccg aggtcaagtt cgaaggagac accctggtta
     5041 atcgtatcga gctcaaaggc atcgatttca aggaggacgg aaatattctg ggccacaagc
     5101 tggagtacaa ttttaacagc cacaatgttt acattactgc cgacaagcag aagaacggca
     5161 tcaaggctaa tttcaagatt cgtcacaatg tggagggagg cgaggagtta ctctcgaaaa
     5221 attatcatct cgaaaacgaa gtggctcggc taaaaaaggg cagtggttct ggagaagagc
     5281 tattatctaa aaactaccac ctcgaaaatg aggtggcacg cttaaaaaag ggaagtggca
     5341 gtggtgaaga gctactatcc aagaattatc atcttgagaa cgaggtagcg cgtttgaaga
     5401 agggttccgg ctcaggagga ggcgacggaa gcgtgcagct ggcagatcat taccaacaga
     5461 acacgcccat tggtgacggt ccagtcctgc tgcctgataa ccactacttg tccacccagt
     5521 cggtgctgtc gaaggacccg aacgagaagc gtgaccacat ggtgctgctg gagttcgtga
     5581 ccgccgcagg aatcaccctc ggcatggatg agctttacaa gggcggagag gaactacttt
     5641 caaagaatta ccacctcgaa aacgaagtag ctcgattaaa gaaaggttca gggtcgggtg
     5701 aagaattact gagtaaaaat tatcatctgg aaaatgaggt agcgagacta aaaaagggga
     5761 gtggttctgg cgaagagttg ctatcgaaaa attatcatct tgagaacgaa gttgctaggc
     5821 tcaaaaaggg ctcaggctca ggccagcggc cgcaccggtc agcttccaag gtgtacgacc
     5881 ccgagcaacg caaacgcatg atcactgggc ctcagtggtg ggctcgctgc aagcaaatga
     5941 acgtgctgga ctccttcatc aactactatg attccgagaa gcacgccgag aacgccgtga
     6001 tttttctgca tggtaacgct gcctccagct acctgtggag gcacgtcgtg cctcacatcg
     6061 agcccgtggc tagatgcatc atccctgatc tgatcggaat gggtaagtcc ggcaagagcg
     6121 ggaatggctc atatcgcctc ctggatcact acaagtacct caccgcttgg ttcgagctgc
     6181 tgaaccttcc aaagaaaatc atctttgtgg gccacgactg gggggcttgt ctggcctttc
     6241 actactccta cgagcaccaa gacaagatca aggccatcgt ccatgctgag agtgtcgtgg
     6301 acgtgatcga gtcctgggac gagtggcctg acatcgagga ggatatcgcc ctgatcaaga
     6361 gcgaagaggg cgagaaaatg gtgcttgaga ataacttctt cgtcgagacc atgctcccaa
     6421 gcaagatcat gcggaaactg gagcctgagg agttcgctgc ctacctggag ccattcaagg
     6481 agaagggcga ggttagacgg cctaccctct cctggcctcg cgagatccct ctcgttaagg
     6541 gaggcaagcc cgacgtcgtc cagattgtcc gcaactacaa cgcctacctt cgggccagcg
     6601 acgatctgcc taagatgttc atcgagtccg accctgggtt cttttccaac gctattgtcg
     6661 agggagctaa gaagttccct aacaccgagt tcgtgaaggt gaagggcctc cacttcagcc
     6721 aggaggacgc tccagatgaa atgggtaagt acatcaagag cttcgtggag cgcgtgctga
     6781 agaacgagca gatgggagtg caggtggaaa ccatctcccc aggagacggg cgcaccttcc
     6841 ccaagcgcgg ccagacctgt gtggtgcact acaccgggat gcttgaagat ggaaagaaag
     6901 tcgattcctc ccgggacaga aacaagccct ttaagtttat gctaggcaag caggaggtga
     6961 tccgaggctg ggaagaaggg gttgcccaga tgagtgtggg tcagagagcc aaactgacta
     7021 tatctccaga ttatgcctat ggtgccactg ggcacccagg catcatccca ccacatgcca
     7081 ctctcgtctt cgatgtggag cttctaaaac cggaataagg gccagggaag aactggctcg
     7141 gaaagaggac cccacatgtt gtagcttcaa gtttaaacgg atccaaccta caaacgggtg
     7201 gaggatcacc ccacccgaca cttcacaatc aaggggtaca atacacaagg gtggaggaac
     7261 accccaccct ccagacacat tacacagaaa tccaatcaaa cagaagcacc atcagggctt
     7321 ctgctaccaa atttatctca aaaaactaca acaaggaatc accatcaggg attccctgtg
     7381 caatatacgt caaacgaggg ccacgacggg aggacgatca cgcctcccga atatcggcat
     7441 gtctggcttt cgaattcagt gcgtggagca tcagcccacg cagccaatca gagtcgaata
     7501 caagtcgact ttcgcgaaga gcatcagcct tcgcgccatt cttacacaaa ccacactctc
     7561 ccctacagga acagcatcag cgttcctgcc cagtacccaa ctcaagaaaa tttatgtccc
     7621 catgcagcat cagcgcatgg gccccaagaa tacatcccca acaaaatcac atccgagcac
     7681 caacagggct cggagtgttg tttcttgtcc aactggacaa accctccatg gaccatcagg
     7741 ccatggactc tcaccaacaa gacaaaaact actcttctcg aagcagcatc agcgcttcga
     7801 aacactcgag catacattgt gcctatttct tgggtggacg atcacgccac ccatgctctc
     7861 acgaatttca aaacacggac aaggacgagc accaccaggg ctcgtcgttc cacgtccaat
     7921 acgattactt acctttcggg atcacgatca cggatcccgc agctacatca cttccactca
     7981 ggacattcaa gcatgcacga tcacggcatg ctccacaagt ctcaaccaca gaaactacca
     8041 aatgggttca gcaccagcga acccactcct acctcaaacc tcttcccaca aaactggcaa
     8101 gcaggatcac cgcttgccca ttccaacata ccaaatcaaa aacaattact ggtacagcat
     8161 cagcgtacca gcccacatct ctcactacta tcaaaaacca aaccgttcag caacagcgaa
     8221 cggtacacac ggaaaaatca actggtttac aaatacgaaa gacgatcacg ctttcgtcca
     8281 gcgcaaacta ttacgaaaaa catccgacgg gaagagcaac agccttcccg cggcggaaaa
     8341 cctcacaaaa acacgacaaa cggatgcacg aacacggcat ccgccgacaa cccacaaact
     8401 tacaaccagg caaacggtgc aggatcaccg caccgtacat caaacacctc agatctcatt
     8461 tcgcgcggga tccagaccac ctcccctgcg agctaagctg gacagccaat gacgggtaag
     8521 agagtgacat ttttcactaa cctaagacag gagggccgtc agagctactg cctaatccaa
     8581 agacgggtaa aagtgataaa aatgtatcac tccaacctaa gacaggcgca gcttccgagg
     8641 gatttgagat ccagacatga taagatacat tgatgagttt ggacaaacca aaactagaat
     8701 gcagtgaaaa aaatgcctta tttgtgaaat ttgtgatgct attgccttat ttgtaaccat
     8761 tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca
     8821 gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga
     8881 ttatgatctg aagttcctat actatttgaa gaataggaac ttcggaatag gaacttcgga
     8941 tctcatatgg ccatatgggg gcgcctagag aaggagtgag ggctggataa agggaggatc
     9001 gaggcggggt cgaacgagga ggttcaaggg ggagagacgg ggcggatgga ggaagaggag
     9061 gcggaggctt agggtgtaca aagggcttga cccagggagg ggggtcaaaa gccaaggctt
     9121 cccaggtcac gatgtagggg acctggtctg ggtgtccatg cgggccaggt gaaaagacct
     9181 tgatcttaac ctgggtgatg aggtctcggt taaaggtgcc gtctcgcggc catccgacgt
     9241 taaaggttgg ccattctgca gagcagaagg taacccaacg tctcttcttg acatctaccg
     9301 actggttgtg agcgatccgc tcgacatctt tccagtgacc taaggtcaaa cttaagggag
     9361 tggtaacagg ctagcccggg cccatatttt cagacaaata cagaaacaca gtcagacaga
     9421 gacaacacag aacgatgctg cagcagacaa gacgcgcggc gcggcttcgg tcccaaaccg
     9481 aaagcaaaaa ttcagacgga ggcgggaact gttttaggtt ctcgtctcct accagaacca
     9541 catatccctc ctctaagggg ggtgcaccaa agagtcctaa acgatcggga ttttaggact
     9601 caggtcgggc cacaaaaacg gcccccgaag tccctgggac gtctcccagg gttgcggccg
     9661 ggtgttccga actcgtcagt tccaccacgg gtccgccaga tacagagcta gttagctaac
     9721 tagtaccgac gcaggcgcat aaaatcagtc atagacacta gacaatcgga cagacacaga
     9781 taagttgctg gccagcttac ctcccggtgg tgggtcggtg gtccctgggc aggggtctcc
     9841 cgatcccgga cgagccccca aatgaaagac ccccgctgac gggtagtcaa tcactcagag
     9901 gagaccctcc caaggaacag cgagaccaca agtcggatgc aactgcaaga gggtttattg
     9961 gatacacggg tacccgggcg actcagtcaa tcggaggact ggcgcgccga gtgaggggtt
    10021 gtgggctctt ttattgagct cggggagcag aagcgcgcga acagaagcga gaagcgaact
    10081 gattggttag ttcaaataag gcacagggtc atttcaggtc cttggggcac cctggaaaca
    10141 tctgatggtt ctctagaaac tgctgagggc tggaccgcat ctggggacca tctgttcttg
    10201 gccctgagcc ggggcaggaa ctgcttacca cagatatcct gtttggccca tattcagctg
    10261 ttccatctgt tcttggccct gagccggggc aggaactgct taccacagat atcctgtttg
    10321 gcccatattc agctgttcca tctgttcctg accttgatct gaacttttct attctcagtt
    10381 atgtattttt ccatgccttg caaaatggcg ttacttaagc tagcttgcca aacctacagg
    10441 tggggtcttt cacatgtata tgtcaaaaat aaaaatcaac taattgacta gtaattaata
    10501 tgactggcat aatgggaaat tgatcctgac agatgcaaac tggcttctca gcagcgcatt
    10561 tatgttgtca actgaggaag gaaacgttaa tgacagaaac tctaagtaat ttccacgttt
    10621 atctattttt atttatacta gctttggtaa caggaatatt gcagcattca tgcacattga
    10681 aacccttatg aaataaaaac atctgtgcat ttaaaatgga attaacattt taaatgttaa
    10741 aaaaagctgg cttagcttcc ccccgccccc tagggcatag aacaagtcaa atgctttata
    10801 tatttgagtt tgggatgtat taggaaactc ctaagagcaa agctagcttg
//
