LOCUS       sequence-350833-       10426 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10426)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10426)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3202
FEATURES             Location/Qualifiers
     source          1..10426
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1199..1223
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3180..3204)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3212..3253
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3364..3952
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3417..3437)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3835..3846)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4024..4257
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4335..4456
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4372..4391)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4426..4445
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4496..4631
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4651..4670)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4652..4670)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4680..4697)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4680..4696)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4689..4711)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4838..5293
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4925..4944)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5135..5156
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5319..5423
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5424..5492,5493..6284)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5642..5661)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6455..7043
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6944..6963
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7197..7214
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7331..7352
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7367..7372,7373..7390,7391..7397)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7405..7421
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7410..7432
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7429..7445
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7429..7445
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7464..7484
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7466..7484
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7512..7738
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7739..7919
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7966..8091
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8584..8817
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9002..9046
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9277..9487
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9299..9495
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9346..9481
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9527..10129
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9527..9546)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10023..10043
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10172..10289
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcatcg attgatcaac
     1201 aagtttgtac aaaaaagttg gcaccatgtt acgtgaggaa gccaccaaga agagcaaaga
     1261 aaaggagcca gggatggctc ttcctcaggg acgcttgact ttcagggatg tggctataga
     1321 gttctctttg gaggagtgga aatgcctgaa ccctgcacag agggctttat acagggctgt
     1381 gatgttggag aactacagga acctggagtt tgtggatagc tctttaaaat ccatgatgga
     1441 gttctcatca accaggcaca gtattacagg agaagtgatc cacacaggga cgttgcaaag
     1501 acataaaagt catcacattg gagatttttg cttcccagaa atgaagaaag atattcatca
     1561 ctttgagttt cagtggcaag aagttgaaag aaatggccat gaagcaccca tgacaaaaat
     1621 caaaaagttg actggtagta cagaccgaag tgatcacagg catgctggaa acaagcctat
     1681 taaagatcag cttggattaa gctttcattc gcatctgcct gaactccaca tgtttcagac
     1741 taaagggaaa attagtaacc aattggacaa gtctatcggt gcttcctcag cttcagaatc
     1801 ccaaagaatt tcttgtaggc tcaaaactca tatttctaat aagtatggga agaatttcct
     1861 ccattcttca ttcacacaaa tacaggaaat atgcatgaga gaaaaacctt gccaaagtaa
     1921 tgagtgtggc aaagccttta attatagctc actcttaagg agacaccaca taacccattc
     1981 aagagagaga gaatataaat gtgatgtatg tggcaagatc tttaatcaga agcaatacat
     2041 tgtatatcat catagatgtc acactggtga gaaaacttac aagtgtaatg agtgtgggaa
     2101 gaccttcact cagatgtcat cccttgtatg ccatcgtaga cttcatactg gagagaaacc
     2161 ttacaagtgt aatgagtgtg gcaagacctt cagtgagaag tcatccctta gatgccatcg
     2221 tagacttcat actggagaga aaccttacaa gtgtaatgag tgtggcaaga cttttggtcg
     2281 aaattcagcc cttgtaattc ataaggcaat tcatactgga gagaaacctt acaagtgtaa
     2341 tgagtgtggc aagaccttca gtcagaaatc atcccttcaa tgccatcata tacttcacac
     2401 tggagagaaa ccttacaaat gtgaagaatg tgacaatgtt tacattcgca gatcacacct
     2461 tgaaagacat aggaagattc atactggaga gggatcatac aaatgtaagg tttgtgacaa
     2521 ggttttccgg agtgattcat accttgcaga acatcagaga gttcatactg gagagaaacc
     2581 atacaagtgt aataaatgtg gcaggagttt cagtcggaag tcatcccttc aataccatca
     2641 tacacttcac actggagaga aaccttacac atgtaatgaa tgtggcaagg tttttagtcg
     2701 aagagaaaac cttgcacgtc atcatagact tcatgctgga gagaaacctt acaaatgtga
     2761 agaatgtgac aaagttttca gtcgcagatc acaccttgaa agacatagga gaattcatac
     2821 tggggaaaaa ccatacaaat gtaaggtttg tgacaaggct ttccggagtg attcatgcct
     2881 tgcaaaccat acgagagttc atactggaga gaaaccttac aagtgtaata aatgtgcgaa
     2941 ggtttttaat caaaaaggaa tccttgcaca acatcagaga gttcatactg gagagaaacc
     3001 ttacaagtgt aatgaatgtg gcaaggtttt taatcaaaaa gcaagccttg caaaacatca
     3061 gagagttcat actgcagaga aaccttacaa gtgtaatgag tgtggcaaag cctttactgg
     3121 acagtcaaca cttattcacc atcaagcaat ccatgggtgt agggaaactt tacaaatgtt
     3181 gccaactttc ttgtacaaag tggttgatat cggtaagcct atccctaacc ctctcctcgg
     3241 tctcgattct acgtagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct
     3301 ttatatatct tgtggaaagg acgacgtgcc ttgtcgaagc cacgttaaac gcgttaagtc
     3361 gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     3421 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     3481 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     3541 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     3601 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     3661 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     3721 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
     3781 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     3841 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     3901 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcgtcgactt
     3961 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     4021 gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt
     4081 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     4141 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     4201 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta
     4261 cgtatagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     4321 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     4381 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     4441 tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc
     4501 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     4561 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     4621 ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca
     4681 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     4741 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     4801 ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta
     4861 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     4921 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     4981 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     5041 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     5101 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     5161 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     5221 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     5281 acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     5341 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     5401 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     5461 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     5521 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     5581 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     5641 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     5701 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     5761 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     5821 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     5881 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     5941 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     6001 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     6061 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     6121 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     6181 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     6241 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     6301 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     6361 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     6421 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     6481 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     6541 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     6601 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     6661 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     6721 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     6781 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     6841 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     6901 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     6961 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     7021 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     7081 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     7141 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     7201 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     7261 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     7321 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
     7381 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
     7441 atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc
     7501 tgcaagctta atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag
     7561 ttagcaacat gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag
     7621 gtggtacgat cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac
     7681 cactgaattg ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg
     7741 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     7801 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     7861 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     7921 tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg
     7981 actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca
     8041 aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag
     8101 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
     8161 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
     8221 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
     8281 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
     8341 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
     8401 aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga
     8461 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     8521 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     8581 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     8641 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     8701 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     8761 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
     8821 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
     8881 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
     8941 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
     9001 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
     9061 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
     9121 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
     9181 attatcgttt cagacccacc tcccaacccc gaggggaccc atgcattgca tctcaattag
     9241 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     9301 cgtctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     9361 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     9421 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     9481 ctaggctttt gcaaaaagct ttctagaggt accaccggag cttaccatga ccgagtacaa
     9541 gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc
     9601 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg
     9661 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg
     9721 ggtcgcggac gacggcgccg ccgtggcggt ctggaccacg ccggagagcg tcgaagcggg
     9781 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc
     9841 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct
     9901 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct
     9961 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc
    10021 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc
    10081 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gccagttaac gaattctaga
    10141 tcttgagaca aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt
    10201 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac
    10261 aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccac
    10321 tttggcgccg gctcgaggcc tgcaggtgca aagatggata aagttttaaa cagagaggaa
    10381 tctttgcagc taatggacct tctaggtctt gaaaggagtg ggaatt
//
