LOCUS       sequence-350835-       10480 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10480)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10480)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3203
FEATURES             Location/Qualifiers
     source          1..10480
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1199..1223
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3234..3258)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3266..3307
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3418..4006
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3471..3491)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3889..3900)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4078..4311
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4389..4510
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4426..4445)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4480..4499
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4550..4685
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4705..4724)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4706..4724)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4734..4751)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4734..4750)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4743..4765)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4892..5347
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4979..4998)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5189..5210
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5373..5477
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5478..5546,5547..6338)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5696..5715)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6509..7097
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6998..7017
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7251..7268
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7385..7406
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7421..7426,7427..7444,7445..7451)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7459..7475
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7464..7486
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7483..7499
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7483..7499
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7518..7538
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7520..7538
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7566..7792
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7793..7973
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8020..8145
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8638..8871
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9056..9100
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9331..9541
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9353..9549
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9400..9535
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9581..10183
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9581..9600)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10077..10097
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10226..10343
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcatcg attgatcaac
     1201 aagtttgtac aaaaaagttg gcatggcaga ggtggtggct gaggtggccg agatgccaac
     1261 acagatgtca ccaggggcag tggagatgtc aacacctatg tcggcagaga tgatggagat
     1321 gtcaacagaa gtgactgaga tgacacctgg ggaggccctt gcctcatccc tcttcttcca
     1381 gcatcaccag ttcatgtgct ctgagtgtgg cagcctctat aacacactgg aggaagtcct
     1441 ctcacaccag gagcagcaca tgcttgctgt ctcagaggag gaggcactga ccacacagaa
     1501 tgttggcctg gagccggagc tggtgccggg tgctgagggg cccttccagt gtggtgaatg
     1561 cagccagctc atcctctccc ctggggagct cctggcccac caggatgccc acctccgaga
     1621 gtctgcaaac cagatccaat accagtgctg ggactgccag gagctgttcc cctcgcccga
     1681 gctgtgggtg gctcatcgaa aggcccagca cctttctgct acggtagctg agccaccagt
     1741 gccacctcct ttgcctcccc caacaccact gcctccacct tctcccccat ccgaagtcaa
     1801 gatggagccc tatgagtgtc ctgagtgctc taccctctgc gccacccctg aggagttctt
     1861 ggagcatcag ggcacccact ttgactccct agagaaagag gagcgcaatg ggttggagga
     1921 ggaggaagag gacgatgagg aggatgaaga agatgatgaa gagatggagg atgaggaggc
     1981 catggcagag gtcggtgatg atgctgtggg aggtgacgag tccacagctg gctgggctca
     2041 gggctgcggg gactgtcccc agcaccagcc ctcagcaggg gctcgccggc aacaccggcg
     2101 gacggctcac agcccggcat ctgccaccca ccccttccac tgcagccagt gtcagcgcag
     2161 tttcagctcc gccaaccggc tgcaggctca tgggcgggcc catgttggtg gcacacatga
     2221 gtgtacaacc tgctccaagg tcttcaagaa agcagcatcg cttgagcagc acttgcggct
     2281 gcatcgcggg gaagcccgct acctctgtgt agactgtggc cgcggctttg gcacagaact
     2341 cacgttggtg gctcaccggc gggcccacac tgccaaccca ttgcatcgct gtcgttgcgg
     2401 caagacgttc agcaacatga ccaagttcct ctaccaccgg cgcactcacg ccggcaaaag
     2461 cggggcacct cccacaggag caacagctcc cccagctcca gcggagccca cccctccacc
     2521 accaccccct gccccacctg cccagctgcc ctgcccacag tgctccaagt cctttgcctc
     2581 agcttcccgg ctgtcccggc accggcgtgc agtacacggg ccccctgaac ggcgtcaccg
     2641 ctgtggggtt tgtggcaagg gcttcaagaa gctgatccac gtgcgcaacc acctgcggac
     2701 acacacgggt gagaggccct tccagtgcca ctcatgtggc aagacctttg cttctttggc
     2761 caacctcagc cgccaccagc tgacccatac gggtgcacgt ccctaccaat gcctggactg
     2821 tggcaagcgc ttcacacaga gctccaacct gcagcagcac cggcggttgc acttgcggcc
     2881 agtcgccttt gcccgcgccc cccgcctccc catcactggt ctctacaaca agagtcccta
     2941 ctactgcggg acttgtggcc gctggttccg cgccatggcg ggcttgcgac tgcatcagcg
     3001 ggtccatgcc cgagctcgga ctttgacgct acagcctccc agatcaccat ctcctgcccc
     3061 acccccacct ccagagcctc aacagactat catgtgcaca gagctggggg agaccatcgc
     3121 catcattgag acatcccagc cactggcgct tgaggacacc ctgcagctgt gccaggctgc
     3181 actgggggcc agtgaagcag gcgggctctt gcagttggac acggccttcg tgtgcccaac
     3241 tttcttgtac aaagtggttg atatcggtaa gcctatccct aaccctctcc tcggtctcga
     3301 ttctacgtag taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat
     3361 atcttgtgga aaggacgact gaatcaagct cccaccttga ccacgcgtta agtcgacaat
     3421 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     3481 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     3541 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     3601 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     3661 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     3721 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     3781 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     3841 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     3901 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     3961 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcgtcg actttaagac
     4021 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     4081 aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct
     4141 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     4201 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     4261 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtacgtata
     4321 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
     4381 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     4441 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     4501 atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct
     4561 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     4621 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     4681 aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc
     4741 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     4801 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     4861 caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
     4921 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     4981 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     5041 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     5101 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     5161 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     5221 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     5281 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
     5341 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     5401 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     5461 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     5521 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     5581 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     5641 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     5701 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     5761 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     5821 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     5881 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     5941 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     6001 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     6061 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     6121 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     6181 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     6241 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     6301 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     6361 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     6421 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     6481 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     6541 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     6601 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     6661 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     6721 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     6781 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     6841 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     6901 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     6961 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     7021 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     7081 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     7141 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     7201 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     7261 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     7321 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     7381 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     7441 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     7501 atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag
     7561 cttaatgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
     7621 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
     7681 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
     7741 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct
     7801 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     7861 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     7921 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
     7981 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
     8041 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
     8101 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
     8161 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
     8221 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
     8281 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
     8341 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
     8401 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
     8461 agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag
     8521 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     8581 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     8641 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     8701 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     8761 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     8821 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     8881 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     8941 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     9001 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     9061 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     9121 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     9181 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     9241 gtttcagacc cacctcccaa ccccgagggg acccatgcat tgcatctcaa ttagtcagca
     9301 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcgtctc
     9361 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     9421 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     9481 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     9541 ttttgcaaaa agctttctag aggtaccacc ggagcttacc atgaccgagt acaagcccac
     9601 ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt
     9661 cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac
     9721 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc
     9781 ggacgacggc gccgccgtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt
     9841 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca
     9901 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac
     9961 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg
    10021 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa
    10081 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg
    10141 accgcgcacc tggtgcatga cccgcaagcc cggtgccagt taacgaattc tagatcttga
    10201 gacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg
    10261 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
    10321 aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat ccactttggc
    10381 gccggctcga ggcctgcagg tgcaaagatg gataaagttt taaacagaga ggaatctttg
    10441 cagctaatgg accttctagg tcttgaaagg agtgggaatt
//
