LOCUS       sequence-350837-       10489 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10489)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10489)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3205
FEATURES             Location/Qualifiers
     source          1..10489
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1199..1223
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3243..3267)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3275..3316
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3427..4015
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3480..3500)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3898..3909)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4087..4320
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4398..4519
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4435..4454)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4489..4508
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4559..4694
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4714..4733)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4715..4733)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4743..4760)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4743..4759)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4752..4774)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4901..5356
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4988..5007)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5198..5219
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5382..5486
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5487..5555,5556..6347)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5705..5724)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6518..7106
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7007..7026
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7260..7277
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7394..7415
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7430..7435,7436..7453,7454..7460)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7468..7484
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7473..7495
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7492..7508
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7492..7508
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7527..7547
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7529..7547
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7575..7801
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7802..7982
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8029..8154
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8647..8880
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9065..9109
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9340..9550
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9362..9558
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9409..9544
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9590..10192
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9590..9609)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10086..10106
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10235..10352
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcatcg attgatcaac
     1201 aagtttgtac aaaaaagttg gcatggcaga ggcaccggct tccccggccc cgctctctcc
     1261 gctcgaagtg gagctggacc cggagttcga gccccagagc cgtccgcgat cctgtacgtg
     1321 gcccctgcaa aggccggagc tccaagcgag ccctgccaag ccctcggggg agacggccgc
     1381 cgactccatg atccccgagg aggaggacga tgaagacgac gaggacggcg ggggacgggc
     1441 cggctcggcc atggcgatcg gcggcggcgg cgggagcggc acgctgggct ccgggctgct
     1501 ccttgaggac tcggcccggg tgctggcacc cggagggcaa gaccccgggt ctgggccagc
     1561 caccgcggcg ggcgggctga gcgggggtac acaggcgctg ctgcagcctc agcaaccgct
     1621 gccaccgccg cagccggggg cggctggggg ctccgggcag ccgaggaaat gttcgtcgcg
     1681 gcggaacgcc tggggaaacc tgtcctacgc ggacctgatc acccgcgcca tcgagagctc
     1741 cccggacaaa cggctcactc tgtcccagat ctacgagtgg atggtgcgtt gcgtgcccta
     1801 cttcaaggat aagggcgaca gcaacagctc tgccggctgg aagaactcca tccggcacaa
     1861 cctgtcactg catagtcgat tcatgcgggt ccagaatgag ggaactggca agagctcttg
     1921 gtggatcatc aaccctgatg gggggaagag cggaaaagcc ccccggcggc gggctgtctc
     1981 catggacaat agcaacaagt ataccaagag ccgtggccgc gcagccaaga agaaggcagc
     2041 cctgcagaca gcccccgaat cagctgacga cagtccctcc cagctctcca agtggcctgg
     2101 cagccccacg tcacgcagca gtgatgagct ggatgcgtgg acggacttcc gttcacgcac
     2161 caattctaac gccagcacag tcagtggccg cctgtcgccc atcatggcaa gcacagagtt
     2221 ggatgaagtc caggacgatg atgcgcctct ctcgcccatg ctctacagca gctcagccag
     2281 cctgtcacct tcagtaagca agccgtgcac ggtggaactg ccacggctga ctgatatggc
     2341 aggcaccatg aatctgaatg atgggctgac tgaaaacctc atggacgacc tgctggataa
     2401 catcacgctc ccgccatccc agccatcgcc cactggggga ctcatgcagc ggagctctag
     2461 cttcccgtat accaccaagg gctcgggcct gggctcccca accagctcct ttaacagcac
     2521 ggtgttcgga ccttcatctc tgaactccct acgccagtct cccatgcaga ccatccaaga
     2581 gaacaagcca gctaccttct cttccatgtc acactatggt aaccagacac tccaggacct
     2641 gctcacttcg gactcactta gccacagcga tgtcatgatg acacagtcgg accccttgat
     2701 gtctcaggcc agcaccgctg tgtctgccca gaattcccgc cggaacgtga tgcttcgcaa
     2761 tgatccgatg atgtcctttg ctgcccagcc taaccaggga agtttggtca atcagaactt
     2821 gctccaccac cagcaccaaa cccagggcgc tcttggtggc agccgtgcct tgtcgaattc
     2881 tgtcagcaac atgggcttga gtgagtccag cagccttggg tcagccaaac accagcagca
     2941 gtctcctgtc agccagtcta tgcaaaccct ctcggactct ctctcaggct cctccttgta
     3001 ctcaactagt gcaaacctgc ccgtcatggg ccatgagaag ttccccagcg acttggacct
     3061 ggacatgttc aatgggagct tggaatgtga catggagtcc attatccgta gtgaactcat
     3121 ggatgctgat gggttggatt ttaactttga ttccctcatc tccacacaga atgttgttgg
     3181 tttgaacgtg gggaacttca ctggtgctaa gcaggcctca tctcagagct gggtgccagg
     3241 ctgcccaact ttcttgtaca aagtggttga tatcggtaag cctatcccta accctctcct
     3301 cggtctcgat tctacgtagt aatgaactag tccgtaactt gaaagtattt cgatttcttg
     3361 gctttatata tcttgtggaa aggacgatta ataagcttct ttgctccaat aacgcgttaa
     3421 gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     3481 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     3541 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     3601 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     3661 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     3721 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     3781 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     3841 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     3901 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     3961 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcgtcga
     4021 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     4081 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg
     4141 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     4201 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     4261 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     4321 gtacgtatag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     4381 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     4441 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     4501 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat
     4561 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     4621 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     4681 cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc
     4741 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     4801 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     4861 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
     4921 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     4981 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     5041 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     5101 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     5161 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     5221 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     5281 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     5341 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     5401 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     5461 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     5521 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     5581 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     5641 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     5701 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     5761 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     5821 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     5881 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     5941 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     6001 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     6061 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     6121 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     6181 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     6241 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     6301 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     6361 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     6421 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     6481 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     6541 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     6601 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     6661 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     6721 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     6781 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     6841 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     6901 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     6961 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     7021 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     7081 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     7141 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     7201 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     7261 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     7321 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     7381 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     7441 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     7501 gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg
     7561 agctgcaagc ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat
     7621 gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt
     7681 aaggtggtac gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg
     7741 aaccactgaa ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa
     7801 cgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     7861 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     7921 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     7981 cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc
     8041 aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg
     8101 ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt
     8161 aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa
     8221 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
     8281 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
     8341 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
     8401 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
     8461 caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg
     8521 agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc
     8581 attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt
     8641 gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc
     8701 gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa
     8761 caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat
     8821 caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct
     8881 ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag
     8941 ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag
     9001 agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca
     9061 agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt
     9121 taacataaca aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt
     9181 aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc
     9241 accattatcg tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat
     9301 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     9361 atgcgtctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     9421 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     9481 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     9541 ggcctaggct tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta
     9601 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc
     9661 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga
     9721 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
     9781 gtgggtcgcg gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc
     9841 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
     9901 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
     9961 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
    10021 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc
    10081 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
    10141 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct
    10201 agatcttgag acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg
    10261 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
    10321 tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc
    10381 cactttggcg ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag
    10441 gaatctttgc agctaatgga ccttctaggt cttgaaagga gtgggaatt
//
