LOCUS       sequence-350841-        9889 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9889)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3209
FEATURES             Location/Qualifiers
     source          1..9889
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1199..1223
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2643..2667)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2675..2716
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2827..3415
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2880..2900)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3298..3309)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3487..3720
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3798..3919
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3835..3854)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3889..3908
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3959..4094
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4114..4133)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4115..4133)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4143..4160)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4143..4159)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4152..4174)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4301..4756
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4388..4407)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4598..4619
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4782..4886
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4887..4955,4956..5747)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5105..5124)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5918..6506
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6407..6426
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6660..6677
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6794..6815
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6830..6835,6836..6853,6854..6860)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6868..6884
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6873..6895
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6892..6908
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6892..6908
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6927..6947
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6929..6947
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6975..7201
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7202..7382
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7429..7554
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8047..8280
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8465..8509
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8740..8950
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8762..8958
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8809..8944
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8990..9592
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8990..9009)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9486..9506
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9635..9752
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcatcg attgatcaac
     1201 aagtttgtac aaaaaagttg gcatggaaac cccaacacct ttgccgcctg tacccgcctc
     1261 cccgacctgc aacccagccc cacggacaat ccagatcgag ttcccacagc atagctcgtc
     1321 gctgctggaa tctctgaacc gccacaggct agagggaaag ttctgtgatg tgtccctcct
     1381 ggtgcagggc cgggaactta gggctcataa agcagtgtta gctgctgcct ctccttactt
     1441 ccatgacaag ctgcttctgg gggatgcgcc tcgtctcact ctaccgagtg tcattgaagc
     1501 cgatgccttc gaggggctgc tccagctcat ttattcaggg cgtctccgcc tgccactgga
     1561 tgctcttcct gctcatctcc ttgtggccag tggccttcaa atgtggcagg tagtagatca
     1621 gtgctcagaa attcttagag aattagaaac ttcaggtggt ggaatttcag cccgtggagg
     1681 aaactcctac catgcccttc tttccactac atcctctaca ggaggctggt gcattcgctc
     1741 ttcgcctttc cagaccccag tacagtcctc tgcttctact gaaagccctg cttccactga
     1801 gagccctgtg ggaggggagg gaagtgaact gggagaagtg ctgcaaattc aggtggaaga
     1861 agaagaggag gaggaggaag atgatgatga tgaggaccag gggtcagcca cactctctca
     1921 gactcctcag ccccagagag tatcaggggt ttttccccgt cctcatggac cccacccact
     1981 gcccatgact gctactcccc gaaagcttcc agagggtgag agtgcaccac ttgagcttcc
     2041 tgcccctcct gcactgcccc ccaaaatctt ctacattaag caggaaccct tcgagcctaa
     2101 ggaggagata tcaggaagcg gaactcagcc tggaggagca aaggaggaaa ccaaagtgtt
     2161 ttctggaggg gacactgaag ggaatgggga gctagggttc ttgttgcctt cagggccagg
     2221 gccaacatct gggggagggg gtccatcctg gaaaccagtg gatcttcatg ggaatgaaat
     2281 cctgtcaggg ggtggaggac ctgggggagc aggccaggcc gtgcatgggc ctgtgaagct
     2341 aggggggaca ccccctgcag atggaaaacg ctttggttgc ctgtgtggga agcggtttgc
     2401 agtgaagcca aagcgtgacc ggcacatcat gctgaccttc agccttcggc cttttggctg
     2461 tggcatctgc aacaagcgct tcaagctgaa gcaccatctg acagagcaca tgaagaccca
     2521 tgctggagcc ctgcatgcct gtccccactg tggccgtcgg ttccgagtcc atgcctgttt
     2581 tctccgccac cgggacctat gcaagggcca gggctgggcc actgcccact ggacttacaa
     2641 gtgcccaact ttcttgtaca aagtggttga tatcggtaag cctatcccta accctctcct
     2701 cggtctcgat tctacgtagt aatgaactag tccgtaactt gaaagtattt cgatttcttg
     2761 gctttatata tcttgtggaa aggacgaatt taatttgtca gttgaacttc aacgcgttaa
     2821 gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     2881 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     2941 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     3001 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     3061 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     3121 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     3181 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     3241 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     3301 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     3361 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcgtcga
     3421 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     3481 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg
     3541 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     3601 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     3661 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     3721 gtacgtatag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     3781 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     3841 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     3901 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat
     3961 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     4021 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     4081 cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc
     4141 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     4201 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     4261 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
     4321 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     4381 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     4441 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     4501 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     4561 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     4621 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     4681 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     4741 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     4801 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     4861 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     4921 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     4981 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     5041 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     5101 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     5161 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     5221 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     5281 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     5341 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     5401 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     5461 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     5521 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     5581 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     5641 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     5701 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     5761 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     5821 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     5881 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     5941 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     6001 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     6061 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     6121 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     6181 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     6241 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     6301 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     6361 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     6421 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     6481 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     6541 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     6601 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     6661 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     6721 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     6781 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     6841 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     6901 gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg
     6961 agctgcaagc ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat
     7021 gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt
     7081 aaggtggtac gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg
     7141 aaccactgaa ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa
     7201 cgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     7261 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     7321 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     7381 cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc
     7441 aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg
     7501 ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt
     7561 aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa
     7621 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
     7681 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
     7741 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
     7801 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
     7861 caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg
     7921 agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc
     7981 attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt
     8041 gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc
     8101 gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa
     8161 caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat
     8221 caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct
     8281 ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag
     8341 ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag
     8401 agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca
     8461 agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt
     8521 taacataaca aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt
     8581 aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc
     8641 accattatcg tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat
     8701 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     8761 atgcgtctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     8821 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     8881 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     8941 ggcctaggct tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta
     9001 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc
     9061 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga
     9121 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
     9181 gtgggtcgcg gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc
     9241 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
     9301 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
     9361 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
     9421 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc
     9481 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
     9541 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct
     9601 agatcttgag acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg
     9661 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
     9721 tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc
     9781 cactttggcg ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag
     9841 gaatctttgc agctaatgga ccttctaggt cttgaaagga gtgggaatt
//
