LOCUS       sequence-350843-        9457 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9457)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9457)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3211
FEATURES             Location/Qualifiers
     source          1..9457
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1199..1223
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2211..2235)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2243..2284
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2395..2983
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2448..2468)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2866..2877)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3055..3288
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3366..3487
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3403..3422)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3457..3476
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3527..3662
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3682..3701)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3683..3701)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3711..3728)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3711..3727)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3720..3742)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3869..4324
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3956..3975)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4166..4187
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4350..4454
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4455..4523,4524..5315)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4673..4692)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5486..6074
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5975..5994
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6228..6245
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6362..6383
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6398..6403,6404..6421,6422..6428)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6436..6452
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6441..6463
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6460..6476
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6460..6476
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6495..6515
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6497..6515
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6543..6769
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6770..6950
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6997..7122
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7615..7848
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8033..8077
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8308..8518
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8330..8526
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8377..8512
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8558..9160
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8558..8577)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9054..9074
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9203..9320
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcatcg attgatcaac
     1201 aagtttgtac aaaaaagttg gcaccatggc tgcccacgtt ggtgcttccc ggactcccca
     1261 agaggtgatg gagcattacg tgagcatgta catccacggg aacctgggga aggcctgcat
     1321 ccccgacacc atccccaacc gcgtgacaga ccacacctgt cccagcggag gccccctctc
     1381 acccagcctc accaccccgc tgcccccgct ggacatctct gtggctgagc agcagcagct
     1441 gggctacatg ccgctgcggg atgattacga gatcgagtat gaccaggatg ccgagacgct
     1501 catcagcggg ctctctgtca actatgatga cgacgacgtg gagatcgagc tgaagcgcgc
     1561 ccacgtggac atgtacgtgc ggaagctgaa agagagacag cggcggaaga acatcgcccg
     1621 tgactacaat ctggtgccag ccttcctggg gaaggacaag aaggagaagg aaaaggcgct
     1681 gaagcgcaag atcaccaagg aggagaagga gctgcgcctg aagctgaggc cgctgtacca
     1741 gttcatgtca tgcaaggagt ttgatgacct ttttgaaaac atgcacaaag aaaaaatgct
     1801 ccgggccaag atccgagaac tgcagcggta ccggcgaaac gggatcacca agatggaaga
     1861 gtcggcagag tacgaggcag cgcggcataa acgggagaag aggaaggaga acaaaaacct
     1921 agccggctcc aaacggggaa aggaggacgg caaagacagc gagttcgccg ccattgagaa
     1981 ccttccaggc ttcgagctcc tgtcagatcg cgagaaggtg ctctgcagct ctttaaactt
     2041 gagtccagcc cgctacgtga ctgtgaagac tattataatt aaagaccacc tccagaagcg
     2101 gcaaggaatc ccctccaaaa gccgccttcc tagctacctg gacaaagtcc taaagaaaag
     2161 gattttgaat ttcctcacag aaagcggctg gatctccagg gacgcgtctt tgccaacttt
     2221 cttgtacaaa gtggttgata tcggtaagcc tatccctaac cctctcctcg gtctcgattc
     2281 tacgtagtaa tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc
     2341 ttgtggaaag gacgatcacg caagccactc ctgctgttca cgcgttaagt cgacaatcaa
     2401 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     2461 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     2521 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     2581 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     2641 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     2701 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     2761 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     2821 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     2881 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     2941 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcgtcgact ttaagaccaa
     3001 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     3061 ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt
     3121 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     3181 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     3241 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt acgtatagta
     3301 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     3361 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     3421 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     3481 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac
     3541 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     3601 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     3661 cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc
     3721 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     3781 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     3841 cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg
     3901 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     3961 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     4021 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     4081 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     4141 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     4201 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     4261 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
     4321 atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     4381 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     4441 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     4501 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     4561 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     4621 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     4681 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     4741 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     4801 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     4861 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     4921 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     4981 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     5041 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     5101 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     5161 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     5221 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     5281 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     5341 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     5401 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     5461 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     5521 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     5581 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     5641 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     5701 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     5761 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     5821 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     5881 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     5941 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     6001 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     6061 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     6121 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     6181 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     6241 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     6301 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     6361 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     6421 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
     6481 attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt
     6541 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
     6601 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
     6661 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
     6721 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
     6781 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     6841 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     6901 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
     6961 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
     7021 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
     7081 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
     7141 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
     7201 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
     7261 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
     7321 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
     7381 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
     7441 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
     7501 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     7561 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     7621 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     7681 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     7741 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     7801 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     7861 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     7921 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     7981 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     8041 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     8101 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     8161 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     8221 tcagacccac ctcccaaccc cgaggggacc catgcattgc atctcaatta gtcagcaacc
     8281 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcgtctcaat
     8341 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     8401 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     8461 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     8521 tgcaaaaagc tttctagagg taccaccgga gcttaccatg accgagtaca agcccacggt
     8581 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc
     8641 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga
     8701 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
     8761 cgacggcgcc gccgtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
     8821 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
     8881 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
     8941 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
     9001 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
     9061 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
     9121 gcgcacctgg tgcatgaccc gcaagcccgg tgccagttaa cgaattctag atcttgagac
     9181 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
     9241 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     9301 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca ctttggcgcc
     9361 ggctcgaggc ctgcaggtgc aaagatggat aaagttttaa acagagagga atctttgcag
     9421 ctaatggacc ttctaggtct tgaaaggagt gggaatt
//
