LOCUS       sequence-350844-       10162 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10162)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10162)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3212
FEATURES             Location/Qualifiers
     source          1..10162
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1199..1223
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2916..2940)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2948..2989
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3100..3688
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3153..3173)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3571..3582)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3760..3993
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4071..4192
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4108..4127)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4162..4181
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4232..4367
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4387..4406)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4388..4406)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4416..4433)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4416..4432)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4425..4447)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4574..5029
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4661..4680)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4871..4892
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5055..5159
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5160..5228,5229..6020)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5378..5397)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6191..6779
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6680..6699
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6933..6950
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7067..7088
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7103..7108,7109..7126,7127..7133)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7141..7157
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7146..7168
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7165..7181
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7165..7181
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7200..7220
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7202..7220
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7248..7474
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7475..7655
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7702..7827
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8320..8553
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8738..8782
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9013..9223
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9035..9231
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9082..9217
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9263..9865
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9263..9282)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9759..9779
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9908..10025
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcatcg attgatcaac
     1201 aagtttgtac aaaaaagttg gcaccatgat gactgctgaa tcacgggaag ccacgggtct
     1261 gtccccacag gctgcacagg agaaggatgg tatcgtaata gtgaaggtgg aagaggaaga
     1321 tgaggaagac cacatgtggg ggcaggattc caccctacag gacacgcctc ctccagaccc
     1381 agagatattc cgccaacgct tcaggcgctt ctgttaccag aacacttttg ggccccgaga
     1441 ggctctcagt cggctgaagg aactttgtca tcagtggctg cggccagaaa taaacaccaa
     1501 ggaacagatc ctggagcttc tggtgctaga gcagtttctt tccatcctgc ccaaggagct
     1561 ccaggtctgg ctgcaggaat accgccccga tagtggagag gaggccgtga cccttctaga
     1621 agacttggag cttgatttat caggacaaca ggtcccaggt caagttcatg gacctgagat
     1681 gctcgcaagg gggatggtgc ctctggatcc agttcaggag tcctcgagct ttgaccttca
     1741 tcacgaggcc acccagtccc acttcaaaca ttcgtctcgg aaaccccgcc tcttacagtc
     1801 acgagctctt cctgctgccc acattcctgc accccctcat gagggtagtc ccagagacca
     1861 ggcgatggca tctgcactat tcacagcgga ttcccaggca atggtgaaga tcgaggacat
     1921 ggctgtgtcc ctcattctgg aggaatgggg atgtcagaat ctggctcgga ggaatctcag
     1981 tagggacaac aggcaggaga attatgggag cgcatttccc cagggtggtg aaaacaggaa
     2041 tgagaacgag gagtcaacct caaaggctga aacctcggaa gattcagcat cacgcgggga
     2101 gacaacagga agatcccaga aagagtttgg agagaaacgt gaccaggagg gcaaaacagg
     2161 agaaagacag cagaaaaacc ctgaggagaa aaccaggaaa gagaaaagag attcagggcc
     2221 agctatagga aaggacaaaa aaaccatcac aggagagaga ggtccaaggg agaaggggaa
     2281 aggattggga agaagcttca gtctgagctc caacttcacc acccctgaag aagttcccac
     2341 gggaacaaag tctcacagat gtgatgaatg tggtaaatgc ttcacgagaa gttcaagcct
     2401 tatccgccat aaaataatcc acactggaga aaagccctat gaatgtagtg agtgtgggaa
     2461 agccttcagt cttaactcca accttgtcct gcatcagagg atccacacag gagagaaacc
     2521 tcatgaatgt aacgagtgtg gcaaggcctt cagccacagt tccaatctca tcctccatca
     2581 gcgcatccac tctggagaga aaccttatga atgtaatgag tgcgggaagg ccttcagcca
     2641 gagctcggac ctcaccaagc atcagagaat tcacacgggg gagaaaccct atgaatgtag
     2701 tgaatgtgga aaagctttca accgaaactc atacctgatt ttgcatcgga gaattcacac
     2761 tcgagaaaag ccctacaagt gcactaagtg tggcaaggcc ttcacccgca gctccaccct
     2821 cactctgcat cacagaatcc atgccagaga gagagcctct gagtacagcc cagcctccct
     2881 tgatgcattt ggcgcgttcc tgaaaagttg tgtgttgcca actttcttgt acaaagtggt
     2941 tgatatcggt aagcctatcc ctaaccctct cctcggtctc gattctacgt agtaatgaac
     3001 tagtccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga
     3061 ggctgataca cagaccgact atgaacgcgt taagtcgaca atcaacctct ggattacaaa
     3121 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     3181 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     3241 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     3301 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     3361 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     3421 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     3481 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
     3541 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     3601 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     3661 cggatctccc tttgggccgc ctccccgcgt cgactttaag accaatgact tacaaggcag
     3721 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     3781 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
     3841 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     3901 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     3961 gaccctttta gtcagtgtgg aaaatctcta gcagtacgta tagtagttca tgtcatctta
     4021 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta
     4081 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     4141 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     4201 ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     4261 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     4321 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc
     4381 aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt
     4441 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     4501 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     4561 aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     4621 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     4681 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     4741 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     4801 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     4861 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     4921 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     4981 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt
     5041 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     5101 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     5161 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     5221 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     5281 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     5341 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     5401 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     5461 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     5521 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     5581 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     5641 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     5701 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     5761 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     5821 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     5881 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     5941 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     6001 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     6061 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     6121 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     6181 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     6241 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     6301 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     6361 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     6421 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     6481 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     6541 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     6601 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     6661 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     6721 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     6781 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     6841 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     6901 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     6961 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     7021 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     7081 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     7141 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
     7201 caattaaccc tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc
     7261 aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga
     7321 gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg
     7381 aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga
     7441 tattgtattt aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg
     7501 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     7561 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     7621 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
     7681 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
     7741 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
     7801 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
     7861 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
     7921 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
     7981 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     8041 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     8101 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     8161 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     8221 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     8281 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     8341 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     8401 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     8461 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     8521 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     8581 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     8641 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     8701 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     8761 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     8821 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     8881 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     8941 aaccccgagg ggacccatgc attgcatctc aattagtcag caaccaggtg tggaaagtcc
     9001 ccaggctccc cagcaggcag aagtatgcaa agcatgcgtc tcaattagtc agcaaccata
     9061 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     9121 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     9181 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctttct
     9241 agaggtacca ccggagctta ccatgaccga gtacaagccc acggtgcgcc tcgccacccg
     9301 cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac
     9361 gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt
     9421 cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgccgt
     9481 ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc
     9541 gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct
     9601 ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga
     9661 ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg
     9721 cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg
     9781 gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat
     9841 gacccgcaag cccggtgcca gttaacgaat tctagatctt gagacaaatg gcagtattca
     9901 tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag
     9961 acataatagc aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa
    10021 attttcgggt ttattacagg gacagcagag atccactttg gcgccggctc gaggcctgca
    10081 ggtgcaaaga tggataaagt tttaaacaga gaggaatctt tgcagctaat ggaccttcta
    10141 ggtcttgaaa ggagtgggaa tt
//
