LOCUS       sequence-350845-       10060 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3213
FEATURES             Location/Qualifiers
     source          1..10060
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1199..1223
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2814..2838)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2846..2887
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2998..3586
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3051..3071)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3469..3480)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3658..3891
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3969..4090
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4006..4025)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4060..4079
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4130..4265
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4285..4304)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4286..4304)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4314..4331)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4314..4330)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4323..4345)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4472..4927
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4559..4578)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4769..4790
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4953..5057
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5058..5126,5127..5918)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5276..5295)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6089..6677
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6578..6597
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6831..6848
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6965..6986
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7001..7006,7007..7024,7025..7031)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7039..7055
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7044..7066
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7063..7079
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7063..7079
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7098..7118
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7100..7118
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7146..7372
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7373..7553
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7600..7725
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8218..8451
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8636..8680
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8911..9121
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8933..9129
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8980..9115
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9161..9763
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9161..9180)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9657..9677
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9806..9923
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcatcg attgatcaac
     1201 aagtttgtac aaaaaagttg gcaccatgga gcaagaaaaa aaactgttgg tctcagattc
     1261 taacagcttt atggagaggg agagtttgaa aagccctttc acaggagata caagtatgaa
     1321 taatttggaa actgttcacc acaataattc taaggcagat aaacttaaag agaaaccttc
     1381 agaatggtct aaaagacata gaccacaaca ttataagcat gaggatgcaa aagaaatgcc
     1441 actgacatgg gttcaagatg agatttggtg tcatgattcc tatgagagtg atggcaagtc
     1501 agagaattgg ggaaatttta tagctaaaga ggaggaaaaa cccaatcacc aggaatggga
     1561 ctcaggagaa cataccaatg cctgtgtcca gcagaattca tcctttgtag acagacccta
     1621 taaatgttcc gaatgttgga aaagcttcag taatagttct catttgcgta ctcaccagag
     1681 gacccactca ggagaaaagc cttataaatg ctctgagtgt gcaaaatgtt tttgtaacag
     1741 ttctcacctg attcagcatc taagaatgca cacaggagag aagccctacc agtgtggtga
     1801 atgtgggaaa agcttcagca atacctccca tcttattatc catgagagaa ctcacacggg
     1861 agagaaaccc tacaaatgtc ccgagtgtgg gaagagattc agcagcagct ctcaccttat
     1921 tcagcatcac agatcacata caggtgaaaa accatatgaa tgttctgtct gcggaaaagg
     1981 cttcagtcac agctatgtcc taatagaaca tcagaggact cacactggag aaaaacctta
     2041 taagtgccct gattgtggga agagttttag tcagagttcc agcctcattc gccaccagcg
     2101 gacacacaca ggtgagaagc cctacaaatg tcttgagtgt gaaaaaagct ttggttgtaa
     2161 ttctactcta ataaaacatc agagaataca tacaggagaa aagccttatc aatgtccaga
     2221 atgtgggaag aattttagtc gtagttcaaa ccttattaca caccagaaaa tgcacacagg
     2281 agagaaatcc tatgaaagtt ctgaatatga ggaaagtttg ggtcagaact gcaatgtgat
     2341 agaagaatgc agaatccagt taggagagaa accatataga tgttgtgaat gtgggaagag
     2401 ttttggcctt agctcccatc tcattagaca tcagagaaca catacaggag aaaaacctta
     2461 cagatgttct gagtgctgga aaactttcag tcagagttcc accctggtga ttcaccaaag
     2521 gacacataca ggagagaaac cttataaatg tcctgattgt ggtgaaagct tcagtcagag
     2581 ctttaacctt atcaggcacc ggaggaccca cataggggaa aaaccttaca aatgtaccag
     2641 ctgtgagaaa tgcttcagca gaagtgccta cctcagtcag catcggaaaa ttcacgtaga
     2701 aaagcctttt gagtctcccg acgttgggga ttttcctcat gaatggactt ggaaaaactg
     2761 ttcaggggaa atgcccttca tctcttcatt ttccgtctca aattcatctt ccttgccaac
     2821 tttcttgtac aaagtggttg atatcggtaa gcctatccct aaccctctcc tcggtctcga
     2881 ttctacgtag taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat
     2941 atcttgtgga aaggacgaaa ctatgcaatt cactttattt gcacgcgtta agtcgacaat
     3001 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     3061 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     3121 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     3181 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     3241 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     3301 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     3361 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     3421 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     3481 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     3541 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcgtcg actttaagac
     3601 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     3661 aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct
     3721 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     3781 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     3841 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtacgtata
     3901 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
     3961 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     4021 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     4081 atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct
     4141 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     4201 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     4261 aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc
     4321 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     4381 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     4441 caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
     4501 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     4561 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     4621 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     4681 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     4741 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     4801 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     4861 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
     4921 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     4981 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     5041 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     5101 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     5161 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     5221 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     5281 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     5341 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     5401 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     5461 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     5521 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     5581 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     5641 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     5701 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     5761 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     5821 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     5881 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     5941 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     6001 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     6061 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     6121 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     6181 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     6241 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     6301 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     6361 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     6421 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     6481 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     6541 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     6601 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     6661 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     6721 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     6781 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     6841 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     6901 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     6961 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     7021 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     7081 atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag
     7141 cttaatgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
     7201 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
     7261 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
     7321 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct
     7381 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     7441 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     7501 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
     7561 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
     7621 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
     7681 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
     7741 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
     7801 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
     7861 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
     7921 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
     7981 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
     8041 agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag
     8101 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     8161 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     8221 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     8281 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     8341 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     8401 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     8461 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     8521 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     8581 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     8641 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     8701 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     8761 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     8821 gtttcagacc cacctcccaa ccccgagggg acccatgcat tgcatctcaa ttagtcagca
     8881 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcgtctc
     8941 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     9001 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     9061 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     9121 ttttgcaaaa agctttctag aggtaccacc ggagcttacc atgaccgagt acaagcccac
     9181 ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt
     9241 cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac
     9301 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc
     9361 ggacgacggc gccgccgtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt
     9421 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca
     9481 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac
     9541 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg
     9601 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa
     9661 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg
     9721 accgcgcacc tggtgcatga cccgcaagcc cggtgccagt taacgaattc tagatcttga
     9781 gacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg
     9841 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
     9901 aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat ccactttggc
     9961 gccggctcga ggcctgcagg tgcaaagatg gataaagttt taaacagaga ggaatctttg
    10021 cagctaatgg accttctagg tcttgaaagg agtgggaatt
//
