LOCUS       sequence-350846-       11029 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11029)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 11029)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3214
FEATURES             Location/Qualifiers
     source          1..11029
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1199..1223
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3783..3807)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3815..3856
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3967..4555
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4020..4040)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4438..4449)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4627..4860
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4938..5059
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4975..4994)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5029..5048
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5099..5234
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5254..5273)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5255..5273)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5283..5300)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5283..5299)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5292..5314)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5441..5896
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5528..5547)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5738..5759
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5922..6026
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6027..6095,6096..6887)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6245..6264)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7058..7646
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7547..7566
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7800..7817
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7934..7955
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7970..7975,7976..7993,7994..8000)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8008..8024
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8013..8035
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8032..8048
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8032..8048
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8067..8087
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8069..8087
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8115..8341
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8342..8522
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8569..8694
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9187..9420
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9605..9649
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9880..10090
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9902..10098
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9949..10084
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             10130..10732
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(10130..10149)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10626..10646
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10775..10892
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcatcg attgatcaac
     1201 aagtttgtac aaaaaagttg gcaccatgac caagtccctg gaatcagtgt cattcaagga
     1261 cgtaactgta gacttcagta gggatgagtg gcaacaatta gaccttgctc agaaaagcct
     1321 gtacagagaa gtgatgctgg aaaactattt caacttgatc tcagtgggat gtcaagttcc
     1381 caaaccagaa gtcatcttca gcttggaaca agaagagcca tgtatgttgg atggtgagat
     1441 ccccagtcag agccgtccag atggggacat tggttttgga cctttacaac agaggatgtc
     1501 tgaagaagtt tctttccagt ctgagattaa tattaatctc ttcacaagag atgacccata
     1561 ttccatttta gaagaattgt ggaaagacga tgaacacaca agaaaatgtg gagaaaacca
     1621 gaacaaacct ttaagtcgtg ttgtcttcat taacaagaaa acactagcta atgacagcat
     1681 ctttgaatat aaggacattg gggaaatagt tcatgtaaac acacacctgg tttcctcaag
     1741 aaaaagaccc cataactgta actcgtgtgg aaagaatttg gagcctatca taaccttata
     1801 taatagaaac aatgcaacag aaaattctga taagactatt ggagatggtg atattttcac
     1861 tcatttgaat tctcatacag aagtgactgc ttgtgaatgt aaccaatgtg ggaaacctct
     1921 gcatcataag caagctctca ttcaacaaca gaaaattcat actagagaga gcctctattt
     1981 gttttctgac tacgtaaatg ttttctcccc gaagtcacat gcctttgcac atgagagtat
     2041 ttgtgctgaa gaaaagcagc atgaatgcca tgaatgtgag gcagtcttca ctcagaagtc
     2101 ccagcttgat ggcagtcaga gggtttatgc aggaatatgc actgaatatg agaaggattt
     2161 ttccctcaag tcaaaccgtc agaaaactcc ttatgagggg aattactata aatgcagtga
     2221 ctatggaaga gcctttatcc agaagtcaga tctgttcaga tgccagagaa ttcattctgg
     2281 agaaaaacct tatgagtaca gtgaatgtga gaaaaacctc cctcagaatt caaaccttaa
     2341 tatacataaa aaaattcata ctggagggaa acactttgaa tgtactgaat gtggaaaagc
     2401 tttcacaagg aaatcaacac taagtatgca tcagaaaatc catacaggag aaaaacctta
     2461 tgtatgtact gaatgtggga aggcctttat ccggaagtca cattttatca cacatgaaag
     2521 aattcatact ggagaaaaac cctatgaatg cagtgactgt gggaaatcct ttattaaaaa
     2581 atcacaactc catgtgcatc agcgaattca cacaggagag aatcccttta tatgttcaga
     2641 atgtgggaag gtcttcactc acaagacaaa tctcattata caccagaaaa ttcatacagg
     2701 agaaagaccc tatatatgta ctgtgtgtgg taaggccttt actgacaggt caaatctcat
     2761 taagcaccaa aaaattcata ctggagagaa accttataaa tgcagcgact gtggaaaatc
     2821 attcacctgg aagtctcggc tcaggataca tcagaagtgt catactggag agagacatta
     2881 tgaatgcagt gaatgtggga aagcattcat tcagaagtca acattaagta tgcaccagag
     2941 aattcataga ggggaaaaac catatgtttg cactgaatgt ggtaaggcct tcttccacaa
     3001 atcccatttt attacacatg agagaattca tactggagag aaaccctatg aatgcagtat
     3061 ttgtgggaaa tccttcacta agaaatcaca gctccacgta catcagcaga ttcacacagg
     3121 agagaaaccc tataggtgtg ctgaatgtgg aaaggctttt actgacagat caaatctctt
     3181 tacacaccag aaaattcaca ctggagagaa accttataaa tgtagtgact gtgggaaagc
     3241 cttcactagg aagtcaggtc tccatataca tcagcaatcc catactggag aaaggcatta
     3301 tgagtgcagt gaatgtggga aagcctttgc aagaaaatca acactaatta tgcatcagag
     3361 aattcataca ggagagaaac cctatatttg taatgaatgt gggaaatcct tcatccagaa
     3421 gtcacactta aatagacaca ggagaattca tactggagag aaaccctatg aatgcagtga
     3481 ctgtggcaag tctttcatta agaaatcaca actccatgag catcatcgaa ttcacacagg
     3541 agagaaacca tatatatgtg ctgagtgtgg aaaggccttc accatcagat caaatcttat
     3601 taaacaccag aaaattcata ctaaacagaa accctataag tgcagtgact tggggaaagc
     3661 cttaaactgg aagccacaac tcagtatgcc tcagaaatct gacaatgggg aagtagagtg
     3721 ctccatgcca caattatggt gtggggactc agaaggtgac caaggccaac tttcttctat
     3781 cttgccaact ttcttgtaca aagtggttga tatcggtaag cctatcccta accctctcct
     3841 cggtctcgat tctacgtagt aatgaactag tccgtaactt gaaagtattt cgatttcttg
     3901 gctttatata tcttgtggaa aggacgagtg cagatcggtc tctatcggct tacgcgttaa
     3961 gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     4021 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     4081 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     4141 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     4201 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     4261 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     4321 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     4381 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     4441 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     4501 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcgtcga
     4561 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     4621 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg
     4681 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     4741 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     4801 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     4861 gtacgtatag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     4921 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     4981 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     5041 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat
     5101 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     5161 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     5221 cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc
     5281 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     5341 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     5401 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
     5461 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     5521 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     5581 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     5641 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     5701 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     5761 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     5821 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     5881 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     5941 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     6001 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     6061 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     6121 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     6181 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     6241 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     6301 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     6361 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     6421 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     6481 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     6541 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     6601 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     6661 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     6721 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     6781 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     6841 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     6901 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     6961 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     7021 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     7081 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     7141 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     7201 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     7261 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     7321 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     7381 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     7441 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     7501 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     7561 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     7621 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     7681 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     7741 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     7801 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     7861 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     7921 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     7981 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     8041 gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg
     8101 agctgcaagc ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat
     8161 gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt
     8221 aaggtggtac gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg
     8281 aaccactgaa ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa
     8341 cgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     8401 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     8461 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     8521 cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc
     8581 aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg
     8641 ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt
     8701 aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa
     8761 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
     8821 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
     8881 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
     8941 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
     9001 caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg
     9061 agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc
     9121 attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt
     9181 gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc
     9241 gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa
     9301 caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat
     9361 caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct
     9421 ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag
     9481 ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag
     9541 agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca
     9601 agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt
     9661 taacataaca aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt
     9721 aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc
     9781 accattatcg tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat
     9841 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     9901 atgcgtctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     9961 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
    10021 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
    10081 ggcctaggct tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta
    10141 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc
    10201 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga
    10261 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
    10321 gtgggtcgcg gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc
    10381 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
    10441 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
    10501 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
    10561 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc
    10621 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
    10681 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct
    10741 agatcttgag acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg
    10801 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
    10861 tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc
    10921 cactttggcg ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag
    10981 gaatctttgc agctaatgga ccttctaggt cttgaaagga gtgggaatt
//
