LOCUS       sequence-350847-       10210 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10210)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10210)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3215
FEATURES             Location/Qualifiers
     source          1..10210
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1199..1223
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2964..2988)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2996..3037
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3148..3736
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3201..3221)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3619..3630)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3808..4041
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4119..4240
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4156..4175)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4210..4229
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4280..4415
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4435..4454)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4436..4454)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4464..4481)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4464..4480)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4473..4495)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4622..5077
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4709..4728)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4919..4940
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5103..5207
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5208..5276,5277..6068)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5426..5445)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6239..6827
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6728..6747
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6981..6998
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7115..7136
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7151..7156,7157..7174,7175..7181)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7189..7205
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7194..7216
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7213..7229
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7213..7229
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7248..7268
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7250..7268
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7296..7522
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7523..7703
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7750..7875
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8368..8601
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8786..8830
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9061..9271
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9083..9279
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9130..9265
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9311..9913
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9311..9330)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9807..9827
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9956..10073
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcatcg attgatcaac
     1201 aagtttgtac aaaaaagttg gcaccatgaa caaatcccag gaacaagtgt cattcaagga
     1261 tgtatgtgtg gacttcactc aggaagagtg gtatctgctg gaccctgctc agaagattct
     1321 atacagagat gtgatcctgg aaaattatag caatcttgtc tcagtagggt attgcattac
     1381 taaaccagaa gtgatcttta agatcgagca aggagaagag ccctggatat tagaaaaagg
     1441 attcccaagc cagtgccacc cagaaaggaa atggaaagtt gatgacgtgt tagagagcag
     1501 ccaggaaaat gaagatgacc atttttggga gcttctattc cacaacaaca aaacagtaag
     1561 tgtagaaaat ggagatagag gaagcaaaac tttcaatttg ggcacagacc ctgtttcttt
     1621 aagaaattat ccctataaaa tatgtgactc atgtgaaatg aatttgaaaa atatttcggg
     1681 cttaattatt agtaaaaaga actgttccag aaagaagcct gatgagttta atgtatgtga
     1741 gaaattgctc cttgatatta ggcatgagaa aatccctatt ggagagaagt cttataaata
     1801 tgatcaaaaa aggaatgcca ttaattatca ccaggatctc agtcagccaa gttttggcca
     1861 atcttttgag tatagtaaaa atggacaagg cttccatgat gaggcagcat tttttacaaa
     1921 taagagatct cagataggag agacagtctg taaatataac gaatgtggaa gaaccttcat
     1981 tgaaagttta aagctgaata tatctcaaag acctcatttg gaaatggagc cgtatggatg
     2041 cagtatttgc gggaagtcct tctgcatgaa tttaaggttt ggacatcaga gagctcttac
     2101 aaaggacaat ccttatgaat ataatgaata tggggaaatc ttctgtgaca attcagcttt
     2161 cattatccat cagggagctt acacaagaaa gattctccgt gaatataaag tgagtgacaa
     2221 aacctgggaa aagtcagctc tcttaaaaca tcaaatagta cacatggggg gaaagtctta
     2281 tgattacaat gaaaatggga gtaatttcag caagaagtca catcttaccc agcttcggag
     2341 agctcacaca ggagaaaaaa cctttgaatg tggtgaatgt gggaaaacct tctgggagaa
     2401 gtcaaacctc actcaacatc agagaacaca cacaggagag aagccctatg aatgtactga
     2461 atgtgggaaa gccttttgcc agaaaccaca cctgaccaac catcagcgaa cacatacagg
     2521 agaaaaaccc tatgaatgta agcaatgtgg aaaaacattc tgtgtgaagt caaacctcac
     2581 tgaacatcag agaacacaca caggggagaa gccctatgaa tgtaatgcat gtgggaaatc
     2641 cttctgccac agatcagccc tcactgtgca tcagagaaca cacacagggg agaaaccgtt
     2701 tatatgtaat gaatgtggaa aatccttctg tgtgaagtca aacctcattg tacatcaaag
     2761 aactcacact ggggagaaac catataagtg taatgaatgt gggaaaacct tctgtgaaaa
     2821 atcagctctc actaaacatc agaggactca cacaggggag aagccgtatg agtgtaatgc
     2881 atgtgggaag acctttagtc agaggtcagt gctcaccaaa catcagagaa ttcacacaag
     2941 ggtgaaagct ctttcaacat ccttgccaac tttcttgtac aaagtggttg atatcggtaa
     3001 gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgaacta gtccgtaact
     3061 tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgacg tatgccgatt
     3121 ttcaccgcca acacgcgtta agtcgacaat caacctctgg attacaaaat ttgtgaaaga
     3181 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     3241 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     3301 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     3361 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     3421 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     3481 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     3541 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     3601 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     3661 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     3721 tgggccgcct ccccgcgtcg actttaagac caatgactta caaggcagct gtagatctta
     3781 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
     3841 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     3901 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     3961 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     4021 cagtgtggaa aatctctagc agtacgtata gtagttcatg tcatcttatt attcagtatt
     4081 tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata
     4141 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     4201 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat
     4261 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     4321 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     4381 attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat
     4441 agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     4501 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     4561 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     4621 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     4681 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     4741 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     4801 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     4861 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     4921 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     4981 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     5041 aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg
     5101 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     5161 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     5221 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     5281 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     5341 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     5401 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     5461 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     5521 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     5581 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     5641 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     5701 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     5761 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     5821 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     5881 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     5941 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     6001 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     6061 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     6121 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     6181 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     6241 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     6301 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     6361 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     6421 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     6481 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     6541 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     6601 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     6661 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     6721 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     6781 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     6841 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     6901 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     6961 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     7021 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     7081 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     7141 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     7201 aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc
     7261 actaaaggga acaaaagctg gagctgcaag cttaatgtag tcttatgcaa tactcttgta
     7321 gtcttgcaac atggtaacga tgagttagca acatgcctta caaggagaga aaaagcaccg
     7381 tgcatgccga ttggtggaag taaggtggta cgatcgtgcc ttattaggaa ggcaacagac
     7441 gggtctgaca tggattggac gaaccactga attgccgcat tgcagagata ttgtatttaa
     7501 gtgcctagct cgatacataa acgggtctct ctggttagac cagatctgag cctgggagct
     7561 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     7621 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     7681 gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa
     7741 ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag
     7801 gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat
     7861 gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg
     7921 ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag
     7981 ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata
     8041 ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat
     8101 acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct
     8161 ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct
     8221 gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata
     8281 taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt
     8341 gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc
     8401 aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc
     8461 tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt
     8521 gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata
     8581 cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac
     8641 tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac
     8701 gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat
     8761 tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg
     8821 ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa aattattcat
     8881 aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa
     8941 tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg
     9001 acccatgcat tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     9061 gcaggcagaa gtatgcaaag catgcgtctc aattagtcag caaccatagt cccgccccta
     9121 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     9181 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     9241 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctttctag aggtaccacc
     9301 ggagcttacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
     9361 cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
     9421 cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
     9481 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgccgtgg cggtctggac
     9541 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga
     9601 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg
     9661 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa
     9721 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
     9781 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
     9841 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc
     9901 cggtgccagt taacgaattc tagatcttga gacaaatggc agtattcatc cacaatttta
     9961 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
    10021 cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt
    10081 attacaggga cagcagagat ccactttggc gccggctcga ggcctgcagg tgcaaagatg
    10141 gataaagttt taaacagaga ggaatctttg cagctaatgg accttctagg tcttgaaagg
    10201 agtgggaatt
//
