LOCUS       sequence-350848-        9097 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9097)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9097)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3216
FEATURES             Location/Qualifiers
     source          1..9097
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1199..1223
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1851..1875)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1883..1924
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2035..2623
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2088..2108)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2506..2517)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2695..2928
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3006..3127
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3043..3062)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3097..3116
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3167..3302
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3322..3341)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3323..3341)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3351..3368)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3351..3367)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3360..3382)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3509..3964
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3596..3615)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3806..3827
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3990..4094
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4095..4163,4164..4955)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4313..4332)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5126..5714
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5615..5634
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5868..5885
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6002..6023
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6038..6043,6044..6061,6062..6068)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6076..6092
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6081..6103
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6100..6116
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6100..6116
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6135..6155
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6137..6155
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6183..6409
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6410..6590
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6637..6762
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7255..7488
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7673..7717
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7948..8158
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        7970..8166
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8017..8152
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8198..8800
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8198..8217)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8694..8714
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8843..8960
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcatcg attgatcaac
     1201 aagtttgtac aaaaaagttg gcatgaaatt caagttacat gtgaattctg ccaggcaata
     1261 caaggacctg tggaatatga gtgatgacaa accctttcta tgtactgcgc ctggatgtgg
     1321 ccagcgtttt accaacgagg atcatttggc tgtccataaa cataaacatg agatgacact
     1381 gaaatttggt ccagcacgta atgacagtgt cattgtggct gatcagaccc caacaccaac
     1441 aagattcttg aaaaactgtg aagaagtggg tttgtttaat gagttggcga gtccatttga
     1501 gaatgaattc aagaaagctt cagaagatga cattaaaaaa atgcctctag atttatcccc
     1561 tcttgcaaca cctatcataa gaagcaaaat tgaggagcct tctgttgtag aaacaactca
     1621 ccaggatagt cctttacctc acccagagtc tactaccagt gatgagaagg aagtaccatt
     1681 ggcacaaact gcacagccca catcagctat tgttcgtcca gcatcattac aggttcccaa
     1741 tgtgctgctt acaagttctg actcaagtgt aattattcag caggcagtac cttcaccaac
     1801 ctcaagtact gtaatcaccc aggcaccatc ctctaacagg ccaattgtgt acccaacttt
     1861 cttgtacaaa gtggttgata tcggtaagcc tatccctaac cctctcctcg gtctcgattc
     1921 tacgtagtaa tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc
     1981 ttgtggaaag gacgacccgg tggtgaccgc tccggtcgaa cgcgttaagt cgacaatcaa
     2041 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     2101 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     2161 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     2221 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     2281 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     2341 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     2401 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     2461 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     2521 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     2581 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcgtcgact ttaagaccaa
     2641 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     2701 ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt
     2761 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     2821 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     2881 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt acgtatagta
     2941 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     3001 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     3061 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     3121 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac
     3181 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     3241 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     3301 cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc
     3361 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     3421 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     3481 cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg
     3541 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     3601 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     3661 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     3721 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     3781 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     3841 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     3901 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
     3961 atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     4021 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     4081 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     4141 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     4201 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     4261 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     4321 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     4381 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     4441 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     4501 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     4561 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     4621 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     4681 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     4741 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     4801 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     4861 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     4921 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     4981 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     5041 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     5101 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     5161 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     5221 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     5281 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     5341 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     5401 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     5461 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     5521 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     5581 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     5641 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     5701 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     5761 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     5821 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     5881 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     5941 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     6001 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     6061 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
     6121 attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt
     6181 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
     6241 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
     6301 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
     6361 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
     6421 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     6481 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     6541 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
     6601 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
     6661 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
     6721 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
     6781 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
     6841 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
     6901 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
     6961 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
     7021 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
     7081 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
     7141 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     7201 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     7261 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     7321 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     7381 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     7441 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     7501 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     7561 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     7621 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     7681 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     7741 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     7801 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     7861 tcagacccac ctcccaaccc cgaggggacc catgcattgc atctcaatta gtcagcaacc
     7921 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcgtctcaat
     7981 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     8041 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     8101 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     8161 tgcaaaaagc tttctagagg taccaccgga gcttaccatg accgagtaca agcccacggt
     8221 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc
     8281 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga
     8341 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
     8401 cgacggcgcc gccgtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
     8461 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
     8521 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
     8581 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
     8641 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
     8701 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
     8761 gcgcacctgg tgcatgaccc gcaagcccgg tgccagttaa cgaattctag atcttgagac
     8821 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
     8881 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     8941 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca ctttggcgcc
     9001 ggctcgaggc ctgcaggtgc aaagatggat aaagttttaa acagagagga atctttgcag
     9061 ctaatggacc ttctaggtct tgaaaggagt gggaatt
//
