LOCUS       sequence-350849-       10867 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10867)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3217
FEATURES             Location/Qualifiers
     source          1..10867
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1199..1223
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3621..3645)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3653..3694
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3805..4393
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3858..3878)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4276..4287)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4465..4698
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4776..4897
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4813..4832)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4867..4886
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4937..5072
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5092..5111)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5093..5111)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5121..5138)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5121..5137)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5130..5152)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5279..5734
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5366..5385)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5576..5597
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5760..5864
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5865..5933,5934..6725)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6083..6102)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6896..7484
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7385..7404
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7638..7655
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7772..7793
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7808..7813,7814..7831,7832..7838)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7846..7862
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7851..7873
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7870..7886
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7870..7886
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7905..7925
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7907..7925
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7953..8179
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8180..8360
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8407..8532
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9025..9258
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9443..9487
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9718..9928
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9740..9936
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9787..9922
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9968..10570
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9968..9987)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10464..10484
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10613..10730
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcatcg attgatcaac
     1201 aagtttgtac aaaaaagttg gcatgccatc tcagaactat gaccttcccc agaagaagca
     1261 ggagaaaatg accaagtttc aggaggctgt gacattcaag gatgtggctg tggtcttctc
     1321 cagggaggaa ctgcgactgc tcgatcttac ccagaggaag ctgtaccgag atgtcatggt
     1381 ggagaacttc aagaacctgg ttgcagtggg gcatcttccc ttccaaccag atatggtatc
     1441 ccaattggaa gcagaagaaa agctttggat gatggaaaca gaaacccaaa gaagcagcaa
     1501 gcatcaaaat aagatggaaa cactccaaaa atttgcatta aaataccttt caaatcaaga
     1561 gctgtcctgc tggcaaatct ggaaacaggt tgcaagtgaa ttaaccaggt gtcttcaggg
     1621 gaagagttcc cagttattac aaggtgactc tattcaggtt tctgaaaatg agaacaatat
     1681 aatgaaccct aaaggagata gctctattta tattgaaaat caagagtttc cattttggag
     1741 aacccagcat tcttgcggga atacatatct gagtgagtca cagattcaga gtagaggtaa
     1801 gcaaattgat gtgaaaaata acctgcaaat acatgaagac ttcatgaaga aatcaccatt
     1861 tcatgagcat attaaaactg acacagaacc aaaaccctgc aaaggtaatg aatatggcaa
     1921 aatcattagt gatggctcca accagaaatt acccttagga gagaaacccc atccatgtgg
     1981 tgagtgtgga aggggcttca gttatagccc aaggcttccc cttcatccga atgttcacac
     2041 aggagaaaaa tgcttcagtc aaagctcaca tctgcgaact catcagagaa ttcacccagg
     2101 agagaaactc aatagatgtc atgaatctgg tgattgcttc aataagagct cttttcattc
     2161 ttatcaatct aatcatacag gagagaagtc ttatagatgc gacagttgcg gcaagggatt
     2221 cagtagcagc acgggtctta tcattcatta cagaactcat actggagaga aaccctataa
     2281 atgcgaggaa tgtggtaaat gctttagtca aagttcaaat tttcagtgcc atcagagagt
     2341 ccacactgaa gaaaaaccat acaaatgcga agagtgtggt aagggcttcg gttggagtgt
     2401 taatctccgt gttcaccaga gggtccacag gggtgagaag ccctataaat gtgaggaatg
     2461 tggtaagggc ttcactcagg ctgcacattt tcacatccat cagagagtcc acactggaga
     2521 gaaaccctac aagtgtgatg tgtgtggtaa gggcttcagc cacaattcac cattaatatg
     2581 ccatcggaga gtccacacag gagagaagcc atacaagtgt gaggcgtgtg ggaaaggctt
     2641 tacccgtaat acagatctgc atattcattt cagagttcac acgggagaga aaccctataa
     2701 atgtaaggag tgtggtaagg gcttcagtca ggcttcaaat cttcaagtcc atcagaatgt
     2761 ccacactggg gagaaacgat tcaagtgtga aacgtgtggg aagggcttca gtcagtcctc
     2821 aaagcttcaa acccatcagc gagtccacac tggagagaaa ccatatagat gtgatgtgtg
     2881 tggtaaggac ttcagttata gttcaaatct taaactacac caagtaattc acactggaga
     2941 aaaaccatat aaatgtgagg aatgtgggaa gggcttcagt tggagatcaa atcttcatgc
     3001 acatcaaaga gttcactcag gagaaaaacc ctataaatgt gagcagtgtg ataagagctt
     3061 cagtcaggcc atagattttc gggtacatca gagagtccat actggagaga agccatacaa
     3121 atgtggtgtc tgtggtaagg gcttcagtca gtcctctggt cttcaatccc atcagagagt
     3181 ccacacgggg gaaaagccat acaaatgtga tgtgtgtgga aagggcttta gatacagttc
     3241 gcagtttata taccatcaga gaggccacac tggagaaaaa ccttacaaat gtgaagagtg
     3301 tgggaaaggc tttggtagga gcttgaatct tcgccatcat cagagggtcc acacgggaga
     3361 gaaaccccat atatgtgagg agtgtggtaa ggccttcagt ctcccctcaa atcttcgagt
     3421 ccacctgggt gttcacacca gggaaaaact ctttaaatgt gaagagtgtg gtaaaggctt
     3481 cagtcagagt gcacgtcttg aagcccatca gagagtccac actggagaaa aaccatacaa
     3541 atgtgacata tgtgataagg acttccgtca ccgttcacgt cttacatatc atcagaaagt
     3601 ccatactggt aaaaagcttt acccaacttt cttgtacaaa gtggttgata tcggtaagcc
     3661 tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgaactagtc cgtaacttga
     3721 aagtatttcg atttcttggc tttatatatc ttgtggaaag gacgagtgtc aatactacgg
     3781 aaatttaaaa cgcgttaagt cgacaatcaa cctctggatt acaaaatttg tgaaagattg
     3841 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     3901 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     3961 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     4021 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     4081 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     4141 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     4201 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     4261 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     4321 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     4381 gccgcctccc cgcgtcgact ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     4441 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
     4501 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     4561 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     4621 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     4681 tgtggaaaat ctctagcagt acgtatagta gttcatgtca tcttattatt cagtatttat
     4741 aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg
     4801 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     4861 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc
     4921 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     4981 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     5041 ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc gccctatagt
     5101 gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     5161 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     5221 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac
     5281 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     5341 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     5401 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     5461 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     5521 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     5581 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     5641 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     5701 gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc
     5761 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     5821 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     5881 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     5941 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     6001 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     6061 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     6121 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     6181 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     6241 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     6301 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     6361 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     6421 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     6481 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     6541 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     6601 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     6661 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     6721 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     6781 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     6841 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     6901 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     6961 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     7021 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     7081 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     7141 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     7201 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     7261 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     7321 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     7381 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     7441 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     7501 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     7561 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     7621 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     7681 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     7741 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     7801 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     7861 aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact
     7921 aaagggaaca aaagctggag ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc
     7981 ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc
     8041 atgccgattg gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc aacagacggg
     8101 tctgacatgg attggacgaa ccactgaatt gccgcattgc agagatattg tatttaagtg
     8161 cctagctcga tacataaacg ggtctctctg gttagaccag atctgagcct gggagctctc
     8221 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     8281 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     8341 agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca
     8401 gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg
     8461 cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg
     8521 tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta
     8581 aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta
     8641 gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg
     8701 ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca
     8761 gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta
     8821 gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccgctgat
     8881 cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa
     8941 agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca
     9001 gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg
     9061 aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg
     9121 tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca
     9181 actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct
     9241 aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc
     9301 tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac
     9361 ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga
     9421 agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc
     9481 aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat
     9541 gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag
     9601 agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc
     9661 catgcattgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     9721 ggcagaagta tgcaaagcat gcgtctcaat tagtcagcaa ccatagtccc gcccctaact
     9781 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     9841 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     9901 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tttctagagg taccaccgga
     9961 gcttaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag
    10021 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga
    10081 tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg
    10141 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gccgtggcgg tctggaccac
    10201 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt
    10261 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc
    10321 caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg
    10381 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc
    10441 cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt
    10501 caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg
    10561 tgccagttaa cgaattctag atcttgagac aaatggcagt attcatccac aattttaaaa
    10621 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
    10681 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
    10741 acagggacag cagagatcca ctttggcgcc ggctcgaggc ctgcaggtgc aaagatggat
    10801 aaagttttaa acagagagga atctttgcag ctaatggacc ttctaggtct tgaaaggagt
    10861 gggaatt
//
