LOCUS       sequence-35086-e         750 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Allows expression of shRNA from U6 promoter and a rescue cDNA from a
            doxycycline (Tet) inducible promoter in the same plasmid. Contains 
            puromycin resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Ma HT, On KF, Tsang YH, Poon RY
  TITLE     An inducible system for expression and validation of the specificity
            of short hairpin RNA in mammalian cells.
  JOURNAL   Nucleic Acids Res. 2007  . 35(4):e22.
  PUBMED    17234679
REFERENCE   2  (bases 1 to 750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2007  . 35(4):e22."
COMMENT     Sequence Label: pKAR1/Pur
FEATURES             Location/Qualifiers
     source          1..750
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             44..643
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(44..63)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     540..560
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 ctcatctccg ggcctttcga cctgcagccc aagctagctt accatgaccg agtacaagcc
       61 cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc
      121 gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt
      181 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
      241 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
      301 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
      361 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
      421 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
      481 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg
      541 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
      601 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgacgcccgc cccacgaccc
      661 gcagcgcccg accgaaagga gcgcacgacc ccatgcatcg ataagcttat ccgacgccgc
      721 catctctagg cccgcgccgg ccccctcgca
//
