LOCUS       sequence-35087-e         745 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Allows expression of shRNA from U6 promoter and a rescue cDNA from a
            doxycycline (Tet) inducible promoter in the same plasmid. Contains 
            puromycin resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Ma HT, On KF, Tsang YH, Poon RY
  TITLE     An inducible system for expression and validation of the specificity
            of short hairpin RNA in mammalian cells.
  JOURNAL   Nucleic Acids Res. 2007  . 35(4):e22.
  PUBMED    17234679
REFERENCE   2  (bases 1 to 745)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2007  . 35(4):e22."
COMMENT     Sequence Label: pKAR1/Pur
FEATURES             Location/Qualifiers
     source          1..745
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             59..82
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             89..112
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     polyA_signal    144..278
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(168..187)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     222..241
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(527..550)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 ttttgacctc catagaagag accgggaccg atccagcctc cgcgggctag ccacaatgga
       61 ctacaaagac gatgacgata aagccatgct ggaagttctg tttcaggggc ccatggctcg
      121 agaattcgag ctcggtaccc ggggatccag acatgataag atacattgat gagtttggac
      181 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
      241 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
      301 ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca
      361 aatgtggtat ggctgattat gatcctgcaa gcctcgtcgt cctggccgga ccacgctatc
      421 tgtgcaaggt ccccggcccc ggacgcgcgc tccatgagca gagcgnccgc cgccgaggcg
      481 aagcttctac cgggtagggg aggcgctttt cccaaggcag tctggagcat gcgctttagc
      541 agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac attccacatc
      601 caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc
      661 tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat
      721 ggaagtagca cgtctcacta gtctc
//
