LOCUS       sequence-350880-        9784 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses rtTA and SspB-mScarlet-I-MYPT (1-169 aa) in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9784)
  AUTHORS   Yamamoto K, Miura H, Ishida M, Mii Y, Kinoshita N, Takada S, Ueno N,
            Sawai S, Kondo Y, Aoki K
  TITLE     Optogenetic relaxation of actomyosin contractility uncovers 
            mechanistic roles of cortical tension during cytokinesis.
  JOURNAL   Nat Commun. 2021 Dec 8;12(1):7145. doi: 10.1038/s41467-021-27458-3.
  PUBMED    34880255
REFERENCE   2  (bases 1 to 9784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-12-8"; volume: "12(1):7145. doi"; pages: " 
            10.1038/s41467-021-27458-3"
COMMENT     Sequence Label: pPBbsr2-rtTA2-TRE-SspB-mScarlet-I-MYPT169
FEATURES             Location/Qualifiers
     source          1..9784
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(635..1426,1427..1495))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1258..1277
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1496..1600)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1626..2081)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1763..1784)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1975..1994
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2208..2230
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2222..2239
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2223..2239
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2249..2268
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2249..2267
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   2297..2331
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     polyA_signal    2653..2877
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(2894..2920)
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             complement(3458..4153)
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet-I
                     /note="matures faster than mScarlet"
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(3733..3752)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     4002..4020
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             complement(4166..4495)
                     /codon_start=1
                     /product="SspB from Haemophilus influenzae, with the 
                     mutations Y11K and A15E to disrupt the natural dimerization
                     of the protein"
                     /label=SspB nano
                     /note="dimerizes with iLID at high affinity to create iLID 
                     nano (Guntas et al., 2015)"
                     /translation="SSPKRPKLLREYYDWLVDNSFTPYLVVDATYLGVNVPVEYVKDGQ
                     IVLNLSASATGNLQLTNDFIQFNARFKGVSRELYIPMGAALAIYARENGDGVMFEPEEI
                     YDELNI"
     promoter        complement(4513..4827)
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     primer_bind     complement(4516..4540)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    4587..4605
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4623..4641
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4658..4676
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4694..4712
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4730..4748
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4765..4783
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4801..4819
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     enhancer        4969..5272
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          5551..6567
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     6491..6513
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     6575..6594
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     misc_feature    7471..8020
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7615..7632)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7842..7861
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     complement(8491..8508)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8497..8721
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8768..8784)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8792..8808
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8816..8822,8823..8840,8841..8846))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8861..8882
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     repeat_region   9116..9178
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(9219..9239)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9219..9237)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9258..9274)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    9282..9298
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9306..9312,9313..9330,9331..9336))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9351..9372
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9489..9506)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(9660..9784,1..464))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9740..9759)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
       61 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
      121 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
      181 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
      241 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
      301 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
      361 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
      421 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
      481 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
      541 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
      601 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
      661 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
      721 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
      781 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
      841 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
      901 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
      961 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     1021 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     1081 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     1141 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     1201 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     1261 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     1321 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     1381 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     1441 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     1501 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     1561 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     1621 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
     1681 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
     1741 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
     1801 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
     1861 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
     1921 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
     1981 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
     2041 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct
     2101 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
     2161 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
     2221 ttgtaaaacg acggccagtg agcgcgcgta atacgactca ctatagggcg aattggggcg
     2281 cgccattcta gattaaccct agaaagatag tctgcgtaaa attgacgcat gcattcttga
     2341 aatattgctc tctctttcta aatagcgcga atccgtcgct gtgcatttag gacatctcag
     2401 tcgccgcttg gagctcccgt gaggcgtgct tgtcaatgcg gtaagtgtca ctgattttga
     2461 actataacga ccgcgtgagt caaaatgacg catgattatc ttttacgtga cttttaagat
     2521 ttaactcata cgataattat attgttattt catgttctac ttacgtgata acttattata
     2581 tatatatttt cttgttatag atatcaacta gaatgctagc atgggcccat ctcgacattg
     2641 attattgact agccatagag cccaccgcat ccccagcatg cctgctattg tcttcccaat
     2701 cctccccctt gctgtcctgc cccaccccac cccccagaat agaatgacac ctactcagac
     2761 aatgcgatgc aatttcctca ttttattagg aaaggacagt gggagtggca ccttccaggg
     2821 tcaaggaagg cacgggggag gggcaaacaa cagatggctg gcaactagaa ggcacagacg
     2881 cgtcgactta atccagagtc aggcgttcca gaggaggcag ctgcagtcta gaagctgctt
     2941 ctatatcaac cccttgccga ttaacttcat tttgaagtag ctcttccatt gcctcctcct
     3001 ccgcaatatc taaaggtgta tctccttcac tgttgacagc ccctacatgt gctccttgac
     3061 caatcaaaaa ctctgcaata tcaagatatc cacaggaagc tgctgcatgt agtggtatcc
     3121 agccttcatt atcaggttga ttaatatttg ctccattttc taccagaaac ttcaccatat
     3181 caacattgtc atcaatgcaa gcctggtgca gggcagtgag tccgtccaca ttggcgtaat
     3241 tgatgtcggc gccgcggtgc agcagcttga ggacctcgtc cgtgtcgccg ctggagcaag
     3301 cagccaggaa gacggcgcca tcgtcgaact tcaccttggt cttctggcgc ttcaccaccg
     3361 gaggctcgag gtccgtctcg gagccgatcc agcgtttcag ctgctcgttc cgcttctgct
     3421 tcgcgtccgc catcttcatg cggccgcctc cctcgagctt gtacagctcg tccatgccgc
     3481 cggtggagtg gcggccctcg gagcgttcgt actgttccac cacggtgtag tcctcgttgt
     3541 gggaggtgat gtccaacttg cggtcaacgt tgtaggcgcc gggcatctgc acgggcttct
     3601 tggccttgta ggtggtcttg aagtccgcca ggtagcggcc tccgtccttc aggcgcaggg
     3661 ccatcttaat gtcgcccttc agcacgccgt cctcggggta caaccgctcg gtggacgctt
     3721 cccagcccat tgtcttcttc tgcattacgg ggccgtcagg agggaagttg gtgccgcgga
     3781 gcttcacctt gtagatcagg gtgccgtcct ccagggaggt gtcctgggtc acggtcacgg
     3841 cgccgccgtc ctcgaagttc atcacgcgct cccacttgaa gccctcgggg aaggactgct
     3901 tatagtagtc ggggatgtcg gcggggtgct tgatgaaggc cctggagccg tacatgaact
     3961 gaggggacag gatgtcccag gagaagggca gggggccacc cttggtcacc ttcagcttgg
     4021 cggtctgggt gccctcgtag gggcggccct cgccctcgcc ctcgatctcg aactcgtggc
     4081 cgttcatgga gccctccatg tgcaccttga accgcatgaa ctccttgatc actgcctcgc
     4141 ccttgctcac catggtgaat tcaccaatat tcagctcgtc atagatttct tctggttcga
     4201 acatcacacc atcgccgttc tcgcgagcgt aaatggccag agcggcaccc atcgggatat
     4261 acagttcacg agacacgccc ttaaagcggg cgttgaactg gataaaatca tttgtcagtt
     4321 gcaggttgcc ggtcgcactt gcagacagat tcagcacgat ctgaccgtct ttcacatact
     4381 ccacgggcac gttcacgccc aggtatgtgg catccaccac cagatatggg gtaaagctgt
     4441 tatcaaccag ccaatcgtaa tattcacgca gcagcttagg gcgtttcggg gagctcatgg
     4501 tggaattctc caggcgatct gacggttcac taaacgagct ctgcttatat aggcctccca
     4561 ccgtacacgc ctacctcgac atacgttctc tatcactgat agggagtaaa ctcgacatac
     4621 gttctctatc actgataggg ataaactcga catacgttct ctatcactga tagggagtaa
     4681 actcgacata cgttctctat cactgatagg gagtaaactc gacatacgtt ctctatcact
     4741 gatagggagt aaactcgaca tcgttctcta tcactgatag ggagtaaact cgacatacgt
     4801 tctctatcac tgatagggag taaactcgac cccccccaaa gcccccaggg atgtaattac
     4861 gtccctcccc cgctaggggg cagcagcgag ccgcccgggg cggcgcgcta gttattaata
     4921 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
     4981 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
     5041 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
     5101 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
     5161 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
     5221 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtcgaggt
     5281 gagccccacg ttctgcttca ctctccccat ctcccccccc tccccacccc caattttgta
     5341 tttatttatt ttttaattat tttgtgcagc gatgggggcg gggggggggg gggnsgcgcg
     5401 ccrggsgggg sggggsgggg sgrggggsgg ggsggggsga ggcggagagg tgcggcggca
     5461 gccaatcaga gcggcgcgct ccgaaagttt ccttttatgg cgaggcggcg gcggcggcgg
     5521 ccctataaaa agcgaagcgc gcggcgggcg ggagtcgctg cgcgctgcct tcgccccgtg
     5581 ccccgctccg ccgccgcctc gcgccgcccg ccccggctct gactgaccgc gttactccca
     5641 caggtgagcg ggcgggacgg cccttctcct ccgggctgta attagcgctt ggtttaatga
     5701 cggcttgttt cttttctgtg gctgcgtgaa agccttgagg ggctccggga gggccctttg
     5761 tgcgggggga gcggctcggg gggtgcgtgc gtgtgtgtgt gcgtggggag cgccgcgtgc
     5821 ggctccgcgc tgcccggcgg ctgtgagcgc tgcgggcgcg gcgcggggct ttgtgcgctc
     5881 cgcagtgtgc gcgaggggag cgcggccggg ggcggtgccc cgcggtgcgg ggggggctgc
     5941 gaggggaaca aaggctgcgt gcggggtgtg tgcgtggggg ggtgagcagg gggtgtgggc
     6001 gcgtcggtcg ggctgcaacc ccccctgcac ccccctcccc gagttgctga gcacggcccg
     6061 gcttcgggtg cggggctccg tacggggcgt ggcgcggggc tcgccgtgcc gggcgggggg
     6121 tggcggcagg tgggggtgcc gggcggggcg gggccgcctc gggccgggga gggctcgggg
     6181 gaggggcgcg gcggcccccg gagcgccggc ggctgtcgag gcgcggcgag ccgcagccat
     6241 tgccttttat ggtaatcgtg cgagagggcg cagggacttc ctttgtccca aatctgtgcg
     6301 gagccgaaat ctgggaggcg ccgccgcacc ccctctagcg ggcgcggggc gaagcggtgc
     6361 ggcgccggca ggaaggaaat gggcggggag ggccttcgtg cgtcgccgcg ccgccgtccc
     6421 cttctccctc tccagcctcg gggctgtccg cggggggacg gctgccttcg ggggggacgg
     6481 ggcagggcgg ggttcggctt ctggcgtgtg accggcggct ctagagcctc tgctaaccat
     6541 gttcatgcct tcttcttttt cctacagctc ctgggcaacg tgctggttat tgtgctgtct
     6601 catcattttg gcaaagaatt gggatccgaa ttaccgccat gtctaggctg gacaagagca
     6661 aaatcatcaa cggggccctc gaactcttga atggggtcgg aatagaagga ttgacaactc
     6721 ggaaactggc ccaaaaactg ggagttgagc agcccacgtt gtattggcat gttaagaaca
     6781 agcgggctct cttggatgca ctgcccatcg agatgctgga tcggcaccac acgcatagtt
     6841 gtccccttga gcccgaaagt tggcaggatt tcttgagaaa taacgcgaag tcatatcgat
     6901 gcgccctgct cagccataga gatggtgcaa aggttcattt gggaacccgc cctaccgaaa
     6961 aacaatatga gaccttggaa aaccaattgg cgttcctctg ccaacaaggt ttcagtttgg
     7021 aaaatgcgct ttatgctctt tctgctgtgg gccattttac tctgggatgc gtactggagg
     7081 agcaagagca ccaagttgct aaagaggaaa gggagacacc aacaacggat agtatgccac
     7141 cgctgttgaa gcaagccata gaactgttcg accgacaggg ggctgaacct gcattccttt
     7201 tcggtctcga attgataatt tgcggactgg aaaagcagtt gaaatgcgag agcggggggc
     7261 ccgcagatgc gctggacgac ttcgaccttg atatgctccc cgccgacgct cttgatgact
     7321 tcgatcttga tatgctgcct gccgacgctc tggatgattt cgacttggac atgctccccg
     7381 ggtaatcgac gggccgcggt aacaattgtt aactaactta agctagcaac ggtttccctc
     7441 tagcgggatc aattccgccc ccccccccta acgttactgg ccgaagccgc ttggaataag
     7501 gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga
     7561 gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg
     7621 ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt
     7681 gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca
     7741 ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc
     7801 agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat
     7861 tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc
     7921 ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga
     7981 accacgggga cgtggttttc ctttgaaaaa cacgataata ccatggtcat gaaaacattt
     8041 aacatttctc aacaagatct agaattagta gaagtagcga cagagaagat tacaatgctt
     8101 tatgaggata ataaacatca tgtgggagcg gcaattcgta cgaaaacagg agaaatcatt
     8161 tcggcagtac atattgaagc gtatatagga cgagtaactg tttgtgcaga agccattgcg
     8221 attggtagtg cagtttcgaa tggacaaaag gattttgaca cgattgtagc tgttagacac
     8281 ccttattctg acgaagtaga tagaagtatt cgagtggtaa gtccttgtgg tatgtgtagg
     8341 gagttgattt cagactatgc accagattgt tttgtgttaa tagaaatgaa tggcaagtta
     8401 gtcaaaacta cgattgaaga actcattcca ctcaaatata cccgaaatta aaagtcgaga
     8461 tgcatcgatg atctagagct cgctgatcag cctcgactgt gccttctagt tgccagccat
     8521 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     8581 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     8641 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
     8701 gggatgcggt gggctctatg gcttctgagg cggaaagaac ctgcagccca agcttggcgt
     8761 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     8821 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     8881 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagcggatct
     8941 taaaagtttt gttactttat agaagaaatt ttgagttttt gttttttttt aataaataaa
     9001 taaacataaa taaattgttt gttgaattta ttattagtat gtaagtgtaa atataataaa
     9061 acttaatatc tattcaaatt aataaataaa cctcgatata cagaccgata aaacacatgc
     9121 gtcaatttta cgcatgatta tctttaacgt acgtcacaat atgattatct ttctagggtt
     9181 aatctagtat acgcgtttaa ttaatccagc ttttgttccc tttagtgagg gttaattgcg
     9241 cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     9301 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     9361 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     9421 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     9481 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     9541 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     9601 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     9661 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     9721 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     9781 tctc
//
