LOCUS       sequence-350899-        1913 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Entry vector containing J23117 anderson promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Goosens VJ, Walker KT, Aragon SM, Singh A, Senthivel VR, Dekker L, 
            Caro-Astorga J, Buat MLA, Song W, Lee KY, Ellis T
  TITLE     Komagataeibacter Tool Kit (KTK): A Modular Cloning System for 
            Multigene Constructs and Programmed Protein Secretion from Cellulose
            Producing Bacteria.
  JOURNAL   ACS Synth Biol. 2021 Dec 17;10(12):3422-3434. doi: 
            10.1021/acssynbio.1c00358. Epub 2021 Nov 12.
  PUBMED    34767345
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00358"; journalName: "ACS Synth Biol"; date: 
            "2021-12-17- 17"; volume: "10"; issue: "12"; pages: "3422-3434"
COMMENT     Sequence Label: KTK_044
FEATURES             Location/Qualifiers
     source          1..1913
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     459..478
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(697..801)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      904..1492
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1393..1412
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        1607..1641
                     /label=BBa_J23117
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.06"
     CDS             complement(join(1749..1913,1..627,628..696))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
       61 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
      121 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
      181 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
      241 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
      301 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
      361 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
      421 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
      481 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
      541 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
      601 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
      661 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
      721 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
      781 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgtca tgaccaaaat
      841 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
      901 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
      961 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     1021 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     1081 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     1141 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     1201 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     1261 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     1321 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     1381 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     1441 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     1501 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     1561 tgcgttatcc cctgattctg tggataaccg tagtcggtct ctgaccttga cagctagctc
     1621 agtcctaggg attgtgctag ctggctgaga ccaagattat caaaaaggat cttcacctag
     1681 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     1741 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     1801 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     1861 tctggcccca gtgctgcaat gataccgcgg gacccacgct caccggctcc aga
//
