LOCUS       sequence-35097-e         737 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 737)
  AUTHORS   Garcia-Pedrajas MD, Nadal M, Kapa LB, Perlin MH, Andrews DL, Gold SE
  TITLE     DelsGate, a robust and rapid gene deletion construction method.
  JOURNAL   Fungal Genet Biol. 2008 Apr . 45(4):379-88.
  PUBMED    18248826
REFERENCE   2  (bases 1 to 737)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet Biol. 2008 Apr . 45(4):379-88."
COMMENT     Sequence Label: pDONRCBX
FEATURES             Location/Qualifiers
     source          1..737
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(37..139)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     protein_bind    complement(284..515)
                     /gene="mutant version of attP"
                     /label=attP2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(628..647)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(706..725)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa
       61 tctcgataac tcaaaaaata cgcccggtag tgatcttatt tcattatggt gaaagttgga
      121 acctcttacg tgccgatcaa cgtctcattt tcgccaaaag ttggcccagg gcttcccggt
      181 atcaacaggg acaccaggat ttatttattc tgcgaagtga tcttccgtca caggtattta
      241 ttcggcgcaa agtgcgtcgg gtgatgctgc caacttagtc gactacaggt cactaatacc
      301 atctaagtag ttgattcata gtgactggat atgttgtgtt ttacagtatt atgtagtctg
      361 ttttttatgc aaaatctaat ttaatatatt gatatttata tcattttacg tttctcgttc
      421 agctttcttg tacaaagttg gcattataag aaagcattgc ttatcaattt gttgcaacga
      481 acaggtcact atcagtcaaa ataaaatcat tatttgccat ccagctgcag ctctggcccg
      541 tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca tgaacaataa
      601 aactgtctgc ttacataaac agtaatacaa ggggtgttat gagccatatt caacgggaaa
      661 cgtcgaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat aaatgggctc
      721 gcgataatgt cgggcaa
//
