LOCUS       sequence-350977-        9761 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of eCFP for auxotrophic selection in the absence of 
            histidine.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9761)
  AUTHORS   Olin-Sandoval V, Yu JSL, Miller-Fleming L, Alam MT, Kamrad S, 
            Correia-Melo C, Haas R, Segal J, Pena Navarro DA, Herrera-Dominguez 
            L, Mendez-Lucio O, Vowinckel J, Mulleder M, Ralser M
  TITLE     Lysine harvesting is an antioxidant strategy and triggers 
            underground polyamine metabolism.
  JOURNAL   Nature. 2019 Aug;572(7768):249-253. doi: 10.1038/s41586-019-1442-6. 
            Epub 2019 Jul 31.
  PUBMED    31367038
REFERENCE   2  (bases 1 to 9761)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-019-1442-6"; journalName: "Nature"; date: "2019-08";
            volume: "572"; issue: "7768"; pages: "249-253"
COMMENT     Sequence Label: pK-eCFP
FEATURES             Location/Qualifiers
     source          1..9761
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     promoter        165..404
                     /gene="S. cerevisiae LYS2"
                     /label=LYS2 promoter
     CDS             405..4583
                     /codon_start=1
                     /gene="S. cerevisiae LYS2"
                     /product="alpha-aminoadipate reductase, required for lysine
                     biosynthesis"
                     /label=LYS2
                     /note="yeast auxotrophic marker"
                     /translation="MTNEKVWIEKLDNPTLSVLPHDFLRPQQEPYTKQATYSLQLPQLD
                     VPHDSFSNKYAVALSVWAALIYRVTGDDDIVLYIANNKILRFNIQPTWSFNELYSTINN
                     ELNKLNSIEANFSFDELAEKIQSCQDLERTPQLFRLAFLENQDFKLDEFKHHLVDFALN
                     LDTSNNAHVLNLIYNSLLYSNERVTIVADQFTQYLTAALSDPSNCITKISLITASSKDS
                     LPDPTKNLGWCDFVGCIHDIFQDNAEAFPERTCVVETPTLNSDKSRSFTYRDINRTSNI
                     VAHYLIKTGIKRGDVVMIYSSRGVDLMVCVMGVLKAGATFSVIDPAYPPARQTIYLGVA
                     KPRGLIVIRAAGQLDQLVEDYINDELEIVSRINSIAIQENGTIEGGKLDNGEDVLAPYD
                     HYKDTRTGVVVGPDSNPTLSFTSGSEGIPKGVLGRHFSLAYYFNWMSKRFNLTENDKFT
                     MLSGIAHDPIQRDMFTPLFLGAQLYVPTQDDIGTPGRLAEWMSKYGCTVTHLTPAMGQL
                     LTAQATTPFPKLHHAFFVGDILTKRDCLRLQTLAENCRIVNMYGTTETQRAVSYFEVKS
                     KNDDPNFLKKLKDVMPAGKGMLNVQLLVVNRNDRTQICGIGEIGEIYVRAGGLAEGYRG
                     LPELNKEKFVNNWFVEKDHWNYLDKDNGEPWRQFWLGPRDRLYRTGDLGRYLPNGDCEC
                     CGRADDQVKIRGFRIELGEIDTHISQHPLVRENITLVRKNADNEPTLITFMVPRFDKPD
                     DLSKFQSDVPKEVETDPIVKGLIGYHLLSKDIRTFLKKRLASYAMPSLIVVMDKLPLNP
                     NGKVDKPKLQFPTPKQLNLVAENTVSETDDSQFTNVEREVRDLWLSILPTKPASVSPDD
                     SFFDLGGHSILATKMIFTLKKKLQVDLPLGTIFKYPTIKAFAAEIDRIKSSGGSSQGEV
                     VENVTANYAEDAKKLVETLPSSYPSREYFVEPNSAEGKTTINVFVTGVTGFLGSYILAD
                     LLGRSPKNYSFKVFAHVRAKDEEAAFARLQKAGITYGTWNEKFASNIKVVLGDLSKSQF
                     GLSDEKWMDLANTVDIIIHNGALVHWVYPYAKLRDPNVISTINVMSLAAVGKPKFFDFV
                     SSTSTLDTEYYFNLSDKLVSEGKPGILESDDLMNSASGLTGGYGQSKWAAEYIIRRAGE
                     RGLRGCIVRPGYVTGASANGSSNTDDFLLRFLKGSVQLGKIPDIENSVNMVPVDHVARV
                     VVATSLNPPKENELAVAQVTGHPRILFKDYLYTLHDYGYDVEIESYSKWKKSLEASVID
                     RNEENALYPLLHMVLDNLPESTKAPELDDRNAVASLKKDTAWTGVDWSNGIGVTPEEVG
                     IYIAFLNKVGFLPPPTHNDKLPLPSIELTQAQISLVASGAGARGSSAAA"
     rep_origin      complement(4671..5126)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4808..4829)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5020..5039
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5252..5274
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5266..5283
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5267..5283
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5290..5309
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5290..5308
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        5335..6001
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     5871..5894
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     CDS             6026..6742
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYIYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGIDELYK"
     terminator      6763..6950
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(6818..6842)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        complement(6988..7006)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7027..7043)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7027..7043)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7040..7062)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7051..7067
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7075..7081,7082..7099,7100..7105))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7120..7141
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7258..7275)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7429..8017)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7509..7528)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8188..8979,8980..9048))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8811..8830
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9049..9153)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    9183..9686
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     9734..9752
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcggcgc gtcgaggaaa actcttcaat agttttgcca gcggaattcc acttgcaatt
      181 acataaaaaa ttccggcggt ttttcgcgtg tgactcaatg tcgaaatacc tgcctaatga
      241 acatgaacat cgcccaaatg tatttgaaga cccgctggga gaagttcaag atatataagt
      301 aacaagcagc caatagtata aaaaaaaatc tgagtttatt acctttcctg gaatttcagt
      361 gaaaaactgc taattataga gagatatcac agagttactc actaatgact aacgaaaagg
      421 tctggataga gaagttggat aatccaactc tttcagtgtt accacatgac tttttacgcc
      481 cacaacaaga accttatacg aaacaagcta catattcgtt acagctacct cagctcgatg
      541 tgcctcatga tagtttttct aacaaatacg ctgtcgcttt gagtgtatgg gctgcattga
      601 tatatagagt aaccggtgac gatgatattg ttctttatat tgcgaataac aaaatcttaa
      661 gattcaatat tcaaccaacg tggtcattta atgagctgta ttctacaatt aacaatgagt
      721 tgaacaagct caattctatt gaggccaatt tttcctttga cgagctagct gaaaaaattc
      781 aaagttgcca agatctggaa aggacccctc agttgttccg tttggccttt ttggaaaacc
      841 aagatttcaa attagacgag ttcaagcatc atttagtgga ctttgctttg aatttggata
      901 ccagtaataa tgcgcatgtt ttgaacttaa tttataacag cttactgtat tcgaatgaaa
      961 gagtaaccat tgttgcggac caatttactc aatatttgac tgctgcgcta agcgatccat
     1021 ccaattgcat aactaaaatc tctctgatca ccgcatcatc caaggatagt ttacctgatc
     1081 caactaagaa cttgggctgg tgcgatttcg tggggtgtat tcacgacatt ttccaggaca
     1141 atgctgaagc cttcccagag agaacctgtg ttgtggagac tccaacacta aattccgaca
     1201 agtcccgttc tttcacttat cgcgacatca accgcacttc taacatagtt gcccattatt
     1261 tgattaaaac aggtatcaaa agaggtgatg tagtgatgat ctattcttct aggggtgtgg
     1321 atttgatggt atgtgtgatg ggtgtcttga aagccggcgc aaccttttca gttatcgacc
     1381 ctgcatatcc cccagccaga caaaccattt acttaggtgt tgctaaacca cgtgggttga
     1441 ttgttattag agctgctgga caattggatc aactagtaga agattacatc aatgatgaat
     1501 tggagattgt ttcaagaatc aattccatcg ctattcaaga aaatggtacc attgaaggtg
     1561 gcaaattgga caatggcgag gatgttttgg ctccatatga tcactacaaa gacaccagaa
     1621 caggtgttgt agttggacca gattccaacc caaccctatc tttcacatct ggttccgaag
     1681 gtattcctaa gggtgttctt ggtagacatt tttccttggc ttattatttc aattggatgt
     1741 ccaaaaggtt caacttaaca gaaaatgata aattcacaat gctgagcggt attgcacatg
     1801 atccaattca aagagatatg tttacaccat tatttttagg tgcccaattg tatgtcccta
     1861 ctcaagatga tattggtaca ccgggccgtt tagcggaatg gatgagtaag tatggttgca
     1921 cagttaccca tttaacacct gccatgggtc aattacttac tgcccaagct actacaccat
     1981 tccctaagtt acatcatgcg ttctttgtgg gtgacatttt aacaaaacgt gattgtctga
     2041 ggttacaaac cttggcagaa aattgccgta ttgttaatat gtacggtacc actgaaacac
     2101 agcgtgcagt ttcttatttc gaagttaaat caaaaaatga cgatccaaac tttttgaaaa
     2161 aattgaaaga tgtcatgcct gctggtaaag gtatgttgaa cgttcagcta ctagttgtta
     2221 acaggaacga tcgtactcaa atatgtggta ttggcgaaat aggtgagatt tatgttcgtg
     2281 caggtggttt ggccgaaggt tatagaggat taccagaatt gaataaagaa aaatttgtga
     2341 acaactggtt tgttgaaaaa gatcactgga attatttgga taaggataat ggtgaacctt
     2401 ggagacaatt ctggttaggt ccaagagata gattgtacag aacgggtgat ttaggtcgtt
     2461 atctaccaaa cggtgactgt gaatgttgcg gtagggctga tgatcaagtt aaaattcgtg
     2521 ggttcagaat cgaattagga gaaatagata cgcacatttc ccaacatcca ttggtaagag
     2581 aaaacattac tttagttcgc aaaaatgccg acaatgagcc aacattgatc acatttatgg
     2641 tcccaagatt tgacaagcca gatgacttgt ctaagttcca aagtgatgtt ccaaaggagg
     2701 ttgaaactga ccctatagtt aagggcttaa tcggttacca tcttttatcc aaggacatca
     2761 ggactttctt aaagaaaaga ttggctagct atgctatgcc ttccttgatt gtggttatgg
     2821 ataaactacc attgaatcca aatggtaaag ttgataagcc taaacttcaa ttcccaactc
     2881 ccaagcaatt aaatttggta gctgaaaata cagtttctga aactgacgac tctcagttta
     2941 ccaatgttga gcgcgaggtt agagacttat ggttaagtat attacctacc aagccagcat
     3001 ctgtatcacc agatgattcg tttttcgatt taggtggtca ttctatcttg gctaccaaaa
     3061 tgatttttac cttaaagaaa aagctgcaag ttgatttacc attgggcaca attttcaagt
     3121 atccaacgat aaaggccttt gccgcggaaa ttgacagaat taaatcatcg ggtggatcat
     3181 ctcaaggtga ggtcgtcgaa aatgtcactg caaattatgc ggaagacgcc aagaaattgg
     3241 ttgagacgct accaagttcg tacccctctc gagaatattt tgttgaacct aatagtgccg
     3301 aaggaaaaac aacaattaat gtgtttgtta ccggtgtcac aggatttctg ggctcctaca
     3361 tccttgcaga tttgttagga cgttctccaa agaactacag tttcaaagtg tttgcccacg
     3421 tcagggccaa ggatgaagaa gctgcatttg caagattaca aaaggcaggt atcacctatg
     3481 gtacttggaa cgaaaaattt gcctcaaata ttaaagttgt attaggcgat ttatctaaaa
     3541 gccaatttgg tctttcagat gagaagtgga tggatttggc aaacacagtt gatataatta
     3601 tccataatgg tgcgttagtt cactgggttt atccatatgc caaattgagg gatccaaatg
     3661 ttatttcaac tatcaatgtt atgagcttag ccgccgtcgg caagccaaag ttctttgact
     3721 ttgtttcctc cacttctact cttgacactg aatactactt taatttgtca gataaacttg
     3781 ttagcgaagg gaagccaggc attttagaat cagacgattt aatgaactct gcaagcgggc
     3841 tcactggtgg atatggtcag tccaaatggg ctgctgagta catcattaga cgtgcaggtg
     3901 aaaggggcct acgtgggtgt attgtcagac caggttacgt aacaggtgcc tctgccaatg
     3961 gttcttcaaa cacagatgat ttcttattga gatttttgaa aggttcagtc caattaggta
     4021 agattccaga tatcgaaaat tccgtgaata tggttccagt agatcatgtt gctcgtgttg
     4081 ttgttgctac gtctttgaat cctcccaaag aaaatgaatt ggccgttgct caagtaacgg
     4141 gtcacccaag aatattattc aaagactact tgtatacttt acacgattat ggttacgatg
     4201 tcgaaatcga aagctattct aaatggaaga aatcattgga ggcgtctgtt attgacagga
     4261 atgaagaaaa tgcgttgtat cctttgctac acatggtctt agacaactta cctgaaagta
     4321 ccaaagctcc ggaactagac gataggaacg ccgtggcatc tttaaagaaa gacaccgcat
     4381 ggacaggtgt tgattggtct aatggaatag gtgttactcc agaagaggtt ggtatatata
     4441 ttgcattttt aaacaaggtt ggatttttac ctccaccaac tcataatgac aaacttccac
     4501 tgccaagtat agaactaact caagcgcaaa taagtctagt tgcttcaggt gctggtgctc
     4561 gtggaagctc cgcagcagct taaggttgag cattacgtat gatatgtgaa acagatccta
     4621 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg aaattgtaag
     4681 cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca
     4741 ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag
     4801 tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg
     4861 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt
     4921 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag
     4981 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc
     5041 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc
     5101 gcttaatgcg ccgctacagg gcgcgtccat tcgccattca ggctgcgcaa ctgttgggaa
     5161 gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca
     5221 aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc
     5281 agtgaattgt aatacgactc actatagggc gaattggagc tccaccgcgg tggctcatta
     5341 tcaatactgc catttcaaag aatacgtaaa taattaatag tagtgatttt cctaacttta
     5401 tttagtcaaa aaattagcct tttaattctg ctgtaacccg tacatgccca aaataggggg
     5461 cgggttacac agaatatata acatcgtagg tgtctgggtg aacagtttat tcctggcatc
     5521 cactaaatat aatggagccc gctttttaag ctggcatcca gaaaaaaaaa gaatcccagc
     5581 accaaaatat tgttttcttc accaaccatc agttcatagg tccattctct tagcgcaact
     5641 acagagaaca ggggcacaaa caggcaaaaa acgggcacaa cctcaatgga gtgatgcaac
     5701 ctgcctggag taaatgatga cacaaggcaa ttgacccacg catgtatcta tctcattttc
     5761 ttacaccttc tattaccttc tgctctctct gatttggaaa aagctgaaaa aaaaggttga
     5821 aaccagttcc ctgaaattat tcccctactt gactaataag tatataaaga cggtaggtat
     5881 tgattgtaat tctgtaaatc tatttcttaa acttcttaaa ttctactttt atagttagtc
     5941 ttttttttag ttttaaaaca ccaagaactt agtttcgaat aaacacacat aaacaaacaa
     6001 aggtgacggt gctggtttaa ttaacatgtc taaaggtgaa gaattattca ctggtgttgt
     6061 cccaattttg gttgaattag atggtgatgt taatggtcac aaattttctg tctccggtga
     6121 aggtgaaggt gatgctactt acggtaaatt gaccttaaaa tttatttgta ctactggtaa
     6181 attgccagtt ccatggccaa ccttagtcac tactttaact tggggtgttc aatgtttttc
     6241 tagataccca gatcatatga aacaacatga ctttttcaag tctgccatgc cagaaggtta
     6301 tgttcaagaa agaactattt ttttcaaaga tgacggtaac tacaagacca gagctgaagt
     6361 caagtttgaa ggtgatacct tagttaatag aatcgaatta aaaggtattg attttaaaga
     6421 agatggtaac attttaggtc acaaattgga atacatttat aactctcaca atgtttacat
     6481 cactgctgac aaacaaaaga atggtatcaa agctaacttc aaaattagac acaacattga
     6541 agatggttct gttcaattag ctgaccatta tcaacaaaat actccaattg gtgatggtcc
     6601 agtcttgtta ccagacaacc attacttatc cactcaatct gccttatcca aagatccaaa
     6661 cgaaaagaga gaccacatgg tcttgttaga atttgttact gctgctggta ttacccatgg
     6721 tattgatgaa ttgtacaaat aaggcgcgcc acttctaaat aagcgaattt cttatgattt
     6781 atgattttta ttattaaata agttataaaa aaaataagtg tatacaaatt ttaaagtgac
     6841 tcttaggttt taaaacgaaa attcttattc ttgagtaact ctttcctgta ggtcaggttg
     6901 ctttctcagg tatagtatga ggtcgctctt attgaccaca cctctaccgg tcgacctgga
     6961 gggggggccc ggtaccagct tttgttccct ttagtgaggg ttaatttcga gcttggcgta
     7021 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     7081 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     7141 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     7201 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     7261 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     7321 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     7381 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     7441 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     7501 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     7561 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     7621 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     7681 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     7741 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     7801 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     7861 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     7921 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     7981 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     8041 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     8101 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     8161 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     8221 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     8281 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     8341 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     8401 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     8461 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     8521 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     8581 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     8641 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     8701 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     8761 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     8821 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     8881 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     8941 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     9001 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     9061 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     9121 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     9181 cggatcgctt gcctgtaact tacacgcgcc tcgtatcttt taatgatgga ataatttggg
     9241 aatttactct gtatttattt atttttatgt tttgtatttg gattttagaa agtaaataaa
     9301 gaaggtagaa gagttacgga atgaagaaaa aaaaataaac aaaggtttaa aaaatttcaa
     9361 caaaaagcgt actttacata tatatttatt agacaagaaa agcagattaa atagatatac
     9421 attcgattaa cgataagtaa aatgtaaaat cacaggattt tcgtgtgtgg tcttctacac
     9481 agacaagatg aaacaattcg gcattaatac ctgagagcag gaagagcaag ataaaaggta
     9541 gtatttgttg gcgatccccc tagagtcttt tacatcttcg gaaaacaaaa actatttttt
     9601 ctttaatttc tttttttact ttctattttt aatttatata tttatattaa aaaatttaaa
     9661 ttataattat ttttatagca cgtgatgaaa aggaccctaa gaaaccatta ttatcatgac
     9721 attaacctat aaaaataggc gtatcacgag gccctttcgt c
//
