LOCUS       sequence-35099-e         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Garcia-Pedrajas MD, Nadal M, Kapa LB, Perlin MH, Andrews DL, Gold SE
  TITLE     DelsGate, a robust and rapid gene deletion construction method.
  JOURNAL   Fungal Genet Biol. 2008 Apr . 45(4):379-88.
  PUBMED    18248826
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet Biol. 2008 Apr . 45(4):379-88."
COMMENT     Sequence Label: pDONRHYG
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     28..47
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     protein_bind    160..391
                     /gene="mutant version of attP"
                     /label=attP2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        536..638
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(705..724)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 tcgcggcctc gacgtttccc gttgaatatg gctcataaca ccccttgtat tactgtttat
       61 gtaagcagac agttttattg ttcatgatga tatattttta tcttgtgcaa tgtaacatca
      121 gagattttga gacacgggcc agagctgcag ctggatggca aataatgatt ttattttgac
      181 tgatagtgac ctgttcgttg caacaaattg ataagcaatg ctttcttata atgccaactt
      241 tgtacaagaa agctgaacga gaaacgtaaa atgatataaa tatcaatata ttaaattaga
      301 ttttgcataa aaaacagact acataatact gtaaaacaca acatatccag tcactatgaa
      361 tcaactactt agatggtatt agtgacctgt agtcgactaa gttggcagca tcacccgacg
      421 cactttgcgc cgaataaata cctgtgacgg aagatcactt cgcagaataa ataaatcctg
      481 gtgtccctgt tgataccggg aagccctggg ccaacttttg gcgaaaatga gacgttgatc
      541 ggcacgtaag aggttccaac tttcaccata atgaaataag atcactaccg ggcgtatttt
      601 ttgagttatc gagattttca ggagctaagg aagctaaaat ggagaaaaaa atcactggat
      661 ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca tttcagtcag
      721 ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg
      781 taaagaaaat aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa
//
