LOCUS       sequence-35100-e         886 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Paz Z, Garcia-Pedrajas MD, Andrews DL, Klosterman SJ, Baeza-Montanez
            L, Gold SE
  TITLE     One step construction of Agrobacterium-Recombination-ready-plasmids 
            (OSCAR), an efficient and robust tool for ATMT based gene deletion 
            construction in fungi.
  JOURNAL   Fungal Genet Biol. 2011 Jul;48(7):677-84. doi: 
            10.1016/j.fgb.2011.02.003. Epub 2011 Mar 6.
  PUBMED    21362493
REFERENCE   2  (bases 1 to 886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.fgb.2011.02.003"; journalName: "Fungal Genet Biol"; date:
            "2011-07"; volume: "48"; issue: "7"; pages: "677-84"
COMMENT     Sequence Label: pOSCAR
FEATURES             Location/Qualifiers
     source          1..886
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(3..234)
                     /gene="mutant version of attP"
                     /label=attP2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        392..494
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(561..580)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 tctacaggtc actaatacca tctaagtagt tggttcatag tgactgcata tgttgtgttt
       61 tacagtatta tgtagtctgt tttttatgca aaatctaatt taatatattg atatttatat
      121 cattttacgt ttctcgttca actttcttgt acaaagttgg cattataaaa aagcattgct
      181 tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt atttggagct
      241 ctagagcgtc gactaagttg gcagcatcac ccgacgcact ttgcgccgaa taaatacctg
      301 tgacggaaga tcacttcgca gaataaataa atcctggtgt ccctgttgat accgggaagc
      361 cctgggccaa cttttggcga aaatgagacg ttgatcggca cgtaagaggt tccaactttc
      421 accataatga aataagatca ctaccgggcg tattttttga gttatcgaga ttttcaggag
      481 ctaaggaagc taaaatggag aaaaaaatca ctggatatac caccgttgat atatcccaat
      541 ggcatcgtaa agaacatttt gaggcatttc agtcagttgc tcaatgtacc tataaccaga
      601 ccgttcagct ggatattacg gcctttttaa agaccgtaaa gaaaaataag cacaagtttt
      661 atccggcctt tattcacatt cttgcccgcc tgatgaatgc tcatccggaa ttccgtatgg
      721 caatgaaaga cggtgagctg gtgatatggg atagtgttca cccttgttac accgttttcc
      781 atgagcaaac tgaaacgttt tcatcgctct ggagtgaata ccacgacgat ttccggcagt
      841 ttctacacat atattcgcaa gatgtggcgt gttacggtga aaacct
//
