LOCUS       sequence-35101-e         771 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Paz Z, Garcia-Pedrajas MD, Andrews DL, Klosterman SJ, Baeza-Montanez
            L, Gold SE
  TITLE     One step construction of Agrobacterium-Recombination-ready-plasmids 
            (OSCAR), an efficient and robust tool for ATMT based gene deletion 
            construction in fungi.
  JOURNAL   Fungal Genet Biol. 2011 Jul;48(7):677-84. doi: 
            10.1016/j.fgb.2011.02.003. Epub 2011 Mar 6.
  PUBMED    21362493
REFERENCE   2  (bases 1 to 771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.fgb.2011.02.003"; journalName: "Fungal Genet Biol"; date:
            "2011-07"; volume: "48"; issue: "7"; pages: "677-84"
COMMENT     Sequence Label: pA-HYG OSCAR
FEATURES             Location/Qualifiers
     source          1..771
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    47..278
                     /gene="mutant version of attP"
                     /label=attP1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 aagctgggta ccgggccccc cctcgaggtc gacgctctag agctccaaat aatgatttta
       61 ttttgactga tagtgacctg ttcgttgcaa caaattgata agcaatgctt ttttataatg
      121 ccaactttgt acaaaaaagt tgaacgagaa acgtaaaatg atataaatat caatatatta
      181 aattagattt tgcataaaaa acagactaca taatactgta aaacacaaca tatgcagtca
      241 ctatgaacca actacttaga tggtattagt gacctgtaga attcctgcag cccaactgat
      301 attgaaggag cattttttgg gcttggctgg agctagtgga ggtcaacaat gaatgcctat
      361 tttggtttag tcgtccaggc ggtgagcaca aaatttgtgt cgtttgacaa gatggttcat
      421 ttaggcaact ggtcagatca gccccacttg tagcagtagc ggcggcgctc gaagtgtgac
      481 tcttattagc agacaggaac gaggacatta ttatcatctg ctgcttggtg cacgataact
      541 tggtgcgttt gtcaagcaag gtaagtggac gacccggtca taccttctta agttcgccct
      601 tcctcccttt atttcagatt caatctgact tacctattct acccaagcat ccaaatgaaa
      661 aagcctgaac tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc
      721 tccgacctga tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt c
//
