LOCUS       sequence-351011-         777 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Codon-optimized bacterial expression plasmid. Expresses 
            GB1-His6-TEV-ALIX(703-868, P801G). P801G mutation facilitates 
            expression in E. coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Elias RD, Zhu Y, Su Q, Ghirlando R, Zhang J, Deshmukh L
  TITLE     Reversible phase separation of ESCRT protein ALIX through tyrosine 
            phosphorylation.
  JOURNAL   Sci Adv. 2023 Jul 14;9(28):eadg3913. doi: 10.1126/sciadv.adg3913. 
            Epub 2023 Jul 14.
  PUBMED    37450591
REFERENCE   2  (bases 1 to 777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2023-07-14"; volume: "9(28):eadg3913. doi"; pages: " 
            10.1126/sciadv.adg3913. Epub 2023 Jul 14"
COMMENT     Sequence Label: GB1-PRD(703-868, P801G)
FEATURES             Location/Qualifiers
     source          1..777
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             169..186
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             187..198
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             238..255
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             256..276
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 atgcaataca aactggcgct gaacggcaaa acgctgaagg gtgaaacgac gaccgaggcc
       61 gttgatgcgg ccacggccga gaaagtgttc aagcagtacg ccaacgacaa cggtgttgat
      121 ggcgaatgga cctacgacga tgcgaccaaa accttcaccg tgaccgaact ggttccgcgc
      181 ggcagtatcg aaggtcgcat ccgcgagaat ctgaaagata gcggtctgtt tctggagcat
      241 caccaccatc accatgaaaa tctgtacttc caaggcgacg aactgctgaa agatctgcag
      301 cagagcattg cccgcgaacc aagcgcccca agcatcccga cgccagccta tcagagtagt
      361 ccggcgggtg gtcatgcgcc gaccccacca acgccggccc cacgtacgat gccgccaacc
      421 aaaccgcaac caccagcgcg tccgccacca ccagttctcc cagccaatcg cgccccaagt
      481 gcgaccgcgc caagcccagt tggtgcgggt accgccgcgc cagcgccaag tcaaacgccg
      541 ggtagtgccc caccaccaca agcgcaaggc ggcccatatc cgacgtaccc gggctatccg
      601 ggctactgcc agatgccaat gccgatgggt tacaacccat acgcgtacgg ccagtataac
      661 atgccgtacc caccggtgta ccaccagagt ccgggccaag ccccataccc gggtccacag
      721 cagccaagct acccgttccc gcagccacca cagcaaagtt actacccgca gcaataa
//
