LOCUS       sequence-351013-        2640 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Codon-optimized bacterial expression plasmid. Expresses ALIX(1-868, 
            P801G)-Strep. P801G mutation facilitates expression in E. coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Elias RD, Zhu Y, Su Q, Ghirlando R, Zhang J, Deshmukh L
  TITLE     Reversible phase separation of ESCRT protein ALIX through tyrosine 
            phosphorylation.
  JOURNAL   Sci Adv. 2023 Jul 14;9(28):eadg3913. doi: 10.1126/sciadv.adg3913. 
            Epub 2023 Jul 14.
  PUBMED    37450591
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2023-07-14"; volume: "9(28):eadg3913. doi"; pages: " 
            10.1126/sciadv.adg3913. Epub 2023 Jul 14"
COMMENT     Sequence Label: ALIX(P801G)-Strep
FEATURES             Location/Qualifiers
     source          1..2640
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2608..2631
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
ORIGIN
        1 catatggcga cctttattag cgtgcagctg aaaaaaacga gcgaagtgga tctggcgaaa
       61 ccgctggtga aatttattca gcagacctat ccgagcggcg gcgaagaaca agcgcagtat
      121 tgtcgcgcag ctgaagagct gagcaaattg cgccgcgccg cggttggtcg cccgctggat
      181 aaacatgaag gcgcgctgga aaccctgctg cgctattatg atcagatttg cagcattgaa
      241 ccgaaatttc cgtttagcga aaatcagatt tgcctgacct ttacctggaa agatgcgttt
      301 gataaaggca gcctgtttgg cggcagcgtg aaactggctc tggctagcct gggctatgaa
      361 aaaagctgcg tgctgtttaa ctgcgcggcc ctggcgagtc agattgcggc ggaacagaac
      421 ctggataacg atgaaggcct gaaaattgca gcgaagcatt accaatttgc gagcggcgcg
      481 tttctgcata ttaaagaaac cgtgctgagc gcgctgagcc gcgaaccgac cgtggatatt
      541 agcccggata ccgtgggcac cctgagcctg attatgctgg cgcaagcaca agaagtgttt
      601 tttctgaaag cgacccgcga taaaatgaaa gatgcgatta ttgcgaaact ggcgaaccaa
      661 gcggcggatt attttggcga tgcgtttaaa cagtgtcagt ataaagatac cctgccgaaa
      721 gaagtgtttc cggtgctggc ggcgaaacat tgtataatgc aagcgaacgc ggaatatcac
      781 caaagtatct tggcgaagca gcagaaaaaa tttggcgaag aaattgcgcg cctgcagcat
      841 gcggcggaac tgattaaaac cgtggcgagc cgctatgatg aatatgtgaa cgtgaaagat
      901 tttagcgata aaattaaccg cgcgctggcg gcggcgaaaa aagataacga ttttatttat
      961 catgatcgcg tgccggatct gaaagacctg gacccgattg gcaaagcgac cctggtgaaa
     1021 agcaccccgg tgaacgtgcc gattagtcag aaatttaccg atctgtttga aaaaatggtg
     1081 ccggtgagcg tgcagcagag cctggcggcg tataatcagc gcaaagcgga tctggtgaac
     1141 cgcagcattg cgcagatgcg cgaagcgacc accctggcga acggcgtgct ggcgagcctg
     1201 aacctgccgg cggcgattga agatgtgagc ggcgataccg tgccgcaatc tatcctgacc
     1261 aaaagccgca gcgtgattga acaaggcggc attcagaccg tggatcagct gattaaagaa
     1321 ctgccggaac tgctgcaacg caatcgcgaa attctggatg aaagcctgcg cctgctggat
     1381 gaagaagaag cgaccgataa cgatctgcgc gcgaaattta aagaacgctg gcagcgcacc
     1441 ccgagcaacg aactgtataa accgctgcgc gcggaaggca ccaactttcg caccgtgctg
     1501 gataaagcgg tgcaagcgga tggccaagtg aaagaatgct atcagagcca tcgcgatacc
     1561 attgtgctgc tgtgcaaacc ggaaccggaa ctgaacgcgg cgattccgag cgcgaacccg
     1621 gcgaaaacca tgcaaggcag cgaagtggtg aacgtgctga aaagcctgct gagcaacctg
     1681 gatgaagtga aaaaagaacg cgaaggcctg gaaaacgatc tgaaaagcgt gaactttgat
     1741 atgacgagca aatttctgac cgcgctggcg caagatggcg tgattaacga agaagcgctg
     1801 agcgtgaccg aactggatcg cgtgtatggc ggcctgacca ccaaagtgca agaaagcctg
     1861 aaaaaacaag aaggcctgct gaaaaacatt caagtgagcc atcaagaatt tagcaaaatg
     1921 aaacagagca acaacgaagc gaacctgcgc gaagaagtgc tgaaaaacct ggcgaccgcg
     1981 tatgataact ttgtggaact ggtggcgaac ctgaaagaag gcaccaaatt ttataacgaa
     2041 ctgaccgaaa ttctggtgcg ctttcagaac aaatgcagcg atattgtgtt tgcgcgcaaa
     2101 accgaacgcg acgaactgct gaaagatctg cagcagagca ttgcccgcga accaagcgcc
     2161 ccaagcatcc cgacgccagc ctatcagagt agtccggcgg gtggtcatgc gccgacccca
     2221 ccaacgccgg ccccacgtac gatgccgcca accaaaccgc aaccaccagc gcgtccgcca
     2281 ccaccagttc tcccagccaa tcgcgcccca agtgcgaccg cgccaagccc agttggtgcg
     2341 ggtaccgccg cgccagcgcc aagtcaaacg ccgggtagtg ccccaccacc acaagcgcaa
     2401 ggcggcccat atccgacgta cccgggctat ccgggctact gccagatgcc aatgccgatg
     2461 ggttacaacc catacgcgta cggccagtat aacatgccgt acccaccggt gtaccaccag
     2521 agtccgggcc aagccccata cccgggtcca cagcagccaa gctacccgtt cccgcagcca
     2581 ccacagcaaa gttactaccc gcagcaatgg agccatccgc agttcgaaaa gtaaggatcc
//
