LOCUS       sequence-351018-         522 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Codon-optimized bacterial expression plasmid. Expresses 
            GB1-His6-TEV-ALIX(800-868)-Strep..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 522)
  AUTHORS   Elias RD, Zhu Y, Su Q, Ghirlando R, Zhang J, Deshmukh L
  TITLE     Reversible phase separation of ESCRT protein ALIX through tyrosine 
            phosphorylation.
  JOURNAL   Sci Adv. 2023 Jul 14;9(28):eadg3913. doi: 10.1126/sciadv.adg3913. 
            Epub 2023 Jul 14.
  PUBMED    37450591
REFERENCE   2  (bases 1 to 522)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2023-07-14"; volume: "9(28):eadg3913. doi"; pages: " 
            10.1126/sciadv.adg3913. Epub 2023 Jul 14"
COMMENT     Sequence Label: GB1-PRD(800-868)-strep
FEATURES             Location/Qualifiers
     source          1..522
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             172..189
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             190..201
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             241..258
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             259..279
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             490..513
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
ORIGIN
        1 catatgcagt acaaactggc gctgaacggc aaaacgctga aaggtgagac caccaccgaa
       61 gcggttgatg cggcgaccgc cgaaaaggtg ttcaaacagt acgccaacga caacggtgtg
      121 gatggcgaat ggacctacga tgacgccacc aaaacgttca ccgtgaccga actggttccg
      181 cgcggtagca ttgaaggccg catccgcgaa aatctgaaag atagcggtct gtttctggaa
      241 catcatcacc accatcacga aaatctgtac ttccaaggcg gcggtccgcc gtatccaacc
      301 tacccgggtt acccgggcta ctgccagatg ccgatgccaa tgggctacaa cccgtacgcc
      361 tacggccagt acaatatgcc gtacccgccg gtgtaccatc agagtccggg ccaagcccca
      421 tatccgggtc cacagcagcc aagttacccg tttccgcagc cgccgcagca gagttactat
      481 ccgcagcagt ggagccatcc gcagttcgag aaataaggat cc
//
