LOCUS       sequence-35102-e         882 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Paz Z, Garcia-Pedrajas MD, Andrews DL, Klosterman SJ, Baeza-Montanez
            L, Gold SE
  TITLE     One step construction of Agrobacterium-Recombination-ready-plasmids 
            (OSCAR), an efficient and robust tool for ATMT based gene deletion 
            construction in fungi.
  JOURNAL   Fungal Genet Biol. 2011 Jul;48(7):677-84. doi: 
            10.1016/j.fgb.2011.02.003. Epub 2011 Mar 6.
  PUBMED    21362493
REFERENCE   2  (bases 1 to 882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.fgb.2011.02.003"; journalName: "Fungal Genet Biol"; date:
            "2011-07"; volume: "48"; issue: "7"; pages: "677-84"
COMMENT     Sequence Label: pNTC OSCAR
FEATURES             Location/Qualifiers
     source          1..882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    64..295
                     /gene="mutant version of attP"
                     /label=attP1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(305..874)
                     /codon_start=1
                     /gene="Streptomyces noursei nat1"
                     /product="nourseothricin acetyltransferase"
                     /label=NrsR
                     /note="confers resistance to nourseothricin"
                     /translation="MTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGFT
                     LREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNRR
                     LTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFTL
                     CGLDTALYDGTASDGEQALYMSMPCP"
ORIGIN
        1 cctcactaag ggaacaaaag ctgggtaccg ggccccccct cgaggtcgac gctctagagc
       61 tccaaataat gattttattt tgactgatag tgacctgttc gttgcaacaa attgataagc
      121 aatgcttttt tataatgcca actttgtaca aaaaagttga acgagaaacg taaaatgata
      181 taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa tactgtaaaa
      241 cacaacatat gcagtcacta tgaaccaact acttagatgg tattagtgac ctgtagaatt
      301 cctctcaggg gcagggcatg ctcatgtaga gcgcctgctc gccgtccgag gcggtgccgt
      361 cgtacagggc ggtgtccagg ccgcagaggg tgaaccccat ccgccggtac gcgtggatcg
      421 ccggtgcgtt gacgttggtg acctccagcc agaggtgccc ggcgccccgc tcgcgggcga
      481 actccgtcgc gagccccatc aacgcgcgcc cgaccccgtg cccccggtgc tccggggcga
      541 cctcgatgtc ctcgacggtc agccggcggt tccagccgga gtacgagacg accacgaagc
      601 ccgccaggtc gccgtcgtcc ccgtacgcga cgaacgtccg ggagtccggg tcgccgtcct
      661 ccccgtcgtc cgattcgtcg tccgattcgt cgtcggggaa caccttggtc aggggcgggt
      721 ccaccggcac ctcccgcagg gtgaagccgt ccccggtggc ggtgacgcgg aagacggtgt
      781 cggtggtgaa ggacccatcc agtgcctcga tggcctcggc gtcccccggg acactggtgc
      841 ggtaccggta agccgtgtcg tcaagagtgg tcatggccat gg
//
