LOCUS       sequence-35105-e         879 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Kaizuka T, Hara T, Oshiro N, Kikkawa U, Yonezawa K, Takehana K, 
            Iemura S, Natsume T, Mizushima N
  TITLE     Tti1 and Tel2 are critical factors in mammalian target of rapamycin 
            complex assembly.
  JOURNAL   J Biol Chem. 2010 Jun 25. 285(26):20109-16.
  PUBMED    20427287
REFERENCE   2  (bases 1 to 879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2010 Jun 25. 285(26):20109-16."
COMMENT     Sequence Label: p3xFLAG-CMV10-hTti1
FEATURES             Location/Qualifiers
     source          1..879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             24..89
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 gtgaaccgtc agaattaacc atggactaca aagaccatga cggtgattat aaagatcatg
       61 acatcgatta caaggatgac gatgacaagc ttgcggccgc gaattcatcg atagatctga
      121 tatcggtacc agtcgactct agaggatcca tggcagtttt tgatactcct gaggaggcct
      181 ttggtgtctt acgtccagtc tgtgttcagc tcacaaagac ccagacagtg gagaatgtgg
      241 agcatctgca gacacgacta caagctgtga gtgacagtgc ccttcaggaa cttcagcagt
      301 acatcctctt ccctctgcga tttaccctga agaccccagg tcccaaaaga gagcgtttga
      361 tccaaagtgt ggtggaatgc ctcacatttg tcctttcttc aacatgtgtg aaagaacagg
      421 agcttctcca ggaactcttt tcagaactct ctgcttgtct gtattcaccc agctcccaaa
      481 aacctgcggc tgtgtccgag gagttgaaat tggctgtgat ccagggactt agcacattaa
      541 tgcactcagc ttatggggac atcattctga ctttttatga gccctccatt ctgccacgtt
      601 taggatttgc tgtatcttta ctgttaggcc ttgcagaaca ggagaaatca aagcaaatta
      661 aaattgctgc cttaaaatgt ttacaggttc tactcttgca gtgtgattgt caggaccatc
      721 caaggtcatt ggatgaactt gaacaaaagc agctggggga tttgtttgcc tcttttttac
      781 ctggaatctc aactgcactg accaggctta tcacaggaga ctttaaacaa ggtcacagca
      841 ttgtcgtatc ttccctaaag atcttttaca agacagtga
//
