LOCUS       sequence-35115-e         939 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 939)
  TITLE     other plasmids
REFERENCE   2  (bases 1 to 939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: BirA in pET28a (w400-2)
FEATURES             Location/Qualifiers
     source          1..939
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(25..42)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ggctttgtta gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg cggccgcaag
       61 cttacctttt tctgcactac gcagggatat ttcaccgccc atccagggtt ttattattcc
      121 atcctgctca agtaataaag ccccctgttt gtctattccg cgtgaaatgc caaatatttc
      181 tttatcacca atgataagtt tcactgggcg attaataaaa ttatccagct tttcccagcg
      241 cgacagataa ggtgccaatc cttcttgttc gaagagttcc aacgcagcac gtaattcacg
      301 tattagcatg gccgccaacg tattacgatc gagattgatc cccgcttcct gcagcgtgat
      361 ccacccctga ttaacgacac tctcttcaac acggcgcatt gccatgttga tcccggctcc
      421 aatgactatt tgcgccgcat cgccagtttt gccagtcagc tccaccagaa tgcctgccag
      481 cttgcgatcc tgcagataga ggtcattagg ccatttaaca cgaactttat ctgcacccag
      541 cttgcgtaat acttccgcca tcacgatacc gataaccaga cttaaaccaa tcgccgccgc
      601 cgggccttgt tccagacgcc agaacatcga caaatataag tttgcgccaa aaggcgaaaa
      661 ccatttccga ccccggcgac cacggccagc ctgctggtat tctgcaatgc aagcatcgcc
      721 cgatttaagc tctccgatac gatcaagaag gtactgattc gtggagtcaa tcactggcag
      781 cacggctaca ctaccgccat ccagctgacc caatatctgt ttagcattaa gtaactggat
      841 aggctcaggc aggctgtatc ctttacccgg aacggtaaag acatcaacgc cccagtcacg
      901 cagtgtctga atgtgtttat taatagccgc ccggctcat
//
