LOCUS       sequence-35116-e        1024 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd gen lentiviral reverse tetracycline-controlled transactivator 3 
            (rtTA3) expression vector, CMV promoter, Hygro .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1024)
  TITLE     other plasmids
REFERENCE   2  (bases 1 to 1024)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLenti CMV rtTA3 Hygro (w785-1)
FEATURES             Location/Qualifiers
     source          1..1024
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     6..30
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    114..138
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             141..848
                     /codon_start=1
                     /product="reverse tetracycline transactivator 3"
                     /label=rtTA3
                     /note="activates expression from Tet-responsive promoters 
                     in the presence of doxycycline"
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPG"
     protein_bind    complement(847..871)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(955..975)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 agcagagctc gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac
       61 ctccatagaa gacaccgact ctagtccagt gtggtggaat tctgcagata tcaacaagtt
      121 tgtacaaaaa agttggcacc atgtctagac tggacaagag caaagtcata aacggagctc
      181 tggaattact caatggtgtc ggtatcgaag gcctgacgac aaggaaactc gctcaaaagc
      241 tgggagttga gcagcctacc ctgtactggc acgtgaagaa caagcgggcc ctgctcgatg
      301 ccctgccaat cgagatgctg gacaggcatc atacccactt ctgccccctg gaaggcgagt
      361 catggcaaga ctttctgcgg aacaacgcca agtcataccg ctgtgctctc ctctcacatc
      421 gcgacggggc taaagtgcat ctcggcaccc gcccaacaga gaaacagtac gaaaccctgg
      481 aaaatcagct cgcgttcctg tgtcagcaag gcttctccct ggagaacgca ctgtacgctc
      541 tgtccgccgt gggccacttt acactgggct gcgtattgga ggaacaggag catcaagtag
      601 caaaagagga aagagagaca cctaccaccg attctatgcc cccacttctg agacaagcaa
      661 ttgagctgtt cgaccggcag ggagccgaac ctgccttcct tttcggcctg gaactaatca
      721 tatgtggcct ggagaaacag ctaaagtgcg aaagcggcgg gccgaccgac gcccttgacg
      781 attttgactt agacatgctc ccagccgatg cccttgacga ttttgacctt gacatgctcc
      841 ccgggtaatc aactttcttg tacaaagtgg ttgatatcca gcacagtggc ggccgctcga
      901 caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
      961 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     1021 tatg
//
