LOCUS       sequence-351179-        1821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor with homologous arms for Rab11b to knock in DExogron 
            (=minilAA7-DExCon) mCherry module.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Gemperle J, Harrison TS, Flett C, Adamson AD, Caswell PT
  TITLE     On demand expression control of endogenous genes with DExCon, 
            DExogron and LUXon reveals differential dynamics of Rab11 family 
            members.
  JOURNAL   Elife. 2022 Jun 16;11. pii: 76651. doi: 10.7554/eLife.76651.
  PUBMED    35708998
REFERENCE   2  (bases 1 to 1821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-06-16"; volume: "11. pii: 76651. doi"; pages: " 
            10.7554/eLife.76651"
COMMENT     Sequence Label: pJET-50-DExogron-mCherry-R11b
FEATURES             Location/Qualifiers
     source          1..1821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    312..330
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    348..366
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    384..402
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    420..438
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    456..474
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    492..510
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    528..546
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 cagtcttccg ccccgccctc ttgagctcgc ccctgatagg ctggcgcgtc cgtcacttca
       61 aaaaggtccg cattccttcc gcctttctcc aggacaccga gggcgaggag ggtggtacca
      121 agcggcgccc accctcagag cactacttcc atctctgatt ggcttcgctg ggtgcccgtc
      181 gctactccac tcgccgctga taggccattc aaccgccgct ctgcgcggcg ccggctccgc
      241 ccccgtcggg tgtttgtggt ggggctgcgg agtcgccgat cccgccggaa gcgccaggac
      301 aaattcttta ctccctatca gtgatagaga acgtatgaag agtttactcc ctatcagtga
      361 tagagaacgt atgcagactt tactccctat cagtgataga gaacgtataa ggagtttact
      421 ccctatcagt gatagagaac gtatgaccag tttactccct atcagtgata gagaacgtat
      481 ctacagttta ctccctatca gtgatagaga acgtatatcc agtttactcc ctatcagtga
      541 tagagaacgt ataagctttg cttatgtaaa ccagggcgcc tataaaagag tgctgatttt
      601 ttgagtaaac ttcaattcca caacactttt gtcttatacc aactttccgt accacttcct
      661 accctcgtaa agatgggatc gggtgggact agtacgcgtg gcttctctga gaccgtggac
      721 ctgatgctga acctgcagtc caataaggag ggctctgtgg atctgaagaa cgtgagcgcc
      781 gtgcctaagg agaagaccac actgaaggac ccatccaagc cccctgccaa ggcacaggtg
      841 gtgggatggc cacccgtgcg gaactacaga aagaatatga tgacccagca gaagacaagc
      901 tccactagtg gcatggtgtc caagggcgaa gaggacaaca tggccatcat caaagaattc
      961 atgcggttca aggtgcacat ggaaggcagc gtgaacggcc acgagttcga gattgaaggc
     1021 gaaggcgagg gcagacctta cgagggaaca cagaccgcca agctgaaagt gaccaaaggc
     1081 ggccctctgc cttttgcctg ggacattctg agccctcagt ttatgtacgg cagcaaggcc
     1141 tacgtgaagc accccgccga tattcccgac tacctgaagc tgagcttccc cgagggcttc
     1201 aagtgggaga gagtgatgaa cttcgaggac ggcggcgtgg tcaccgtgac tcaagatagc
     1261 tctctgcagg acggcgagtt catctacaaa gtgaagctgc ggggcaccaa ctttccctct
     1321 gatggccccg tgatgcagaa aaagaccatg ggctgggaag ccagcagcga gagaatgtac
     1381 cctgaagatg gcgccctgaa gggcgagatc aagcagcggc tgaaactgaa ggatggcggc
     1441 cactacgacg ccgaagtgaa aaccacctac aaggccaaga aacccgtgca gctgcctggc
     1501 gcctacaacg tgaacatcaa gctggacatc accagccaca acgaggacta caccatcgtg
     1561 gaacagtacg agagagccga aggcagacac agcacaggcg gaatggacga gctgtacaag
     1621 ggaaccggtg ctggaagtgg tacccgggac gacgagtacg actacctatt caaaggtgcg
     1681 gccggtgggg cacagacggg cgaagtcgtg gcggcgaggc ggcgggcaga cgggccaagg
     1741 ccgcgctcca ggccgctggg cccaggccgg agcccggggc ttggggcccg gcgggtcagg
     1801 cagccgggaa ggccggagcc g
//
