LOCUS       sequence-35119-e         862 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 862)
  TITLE     other plasmids
REFERENCE   2  (bases 1 to 862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLenti CMVTRE3G eGFP Blast (w818-1)
FEATURES             Location/Qualifiers
     source          1..862
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      18..27
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    39..91
                     /label=MCS
                     /note="multiple cloning site"
     protein_bind    complement(120..144)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(190..568)
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     primer_bind     complement(257..281)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    326..344
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    362..380
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    398..416
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    434..452
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    470..488
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    506..524
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    542..560
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    595..712
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ctcctcgccc ttgctcacca tggtggcgac cggccggtgg atcccgggcc cgcggtaccg
       61 tcgactgcag aattcgaagc ttgagctcga gatccagtcg actgaattgg ttcctttaaa
      121 gcctgctttt ttgtacaaac ttgttgatat ctgcagaatt ccaccacact ggactagtcg
      181 acgaaccggt ttacgagggt aggaagtggt acggaaagtt ggtataagac aaaagtgttg
      241 tggaattgct ccaggcgatc tgacggttca ctaaacgagc tctgctttta taggcgccca
      301 ccgtacacgc ctaaagctta tacgttctct atcactgata gggagtaaac tggatatacg
      361 ttctctatca ctgataggga gtaaactgta gatacgttct ctatcactga tagggagtaa
      421 actggtcata cgttctctat cactgatagg gagtaaactc cttatacgtt ctctatcact
      481 gatagggagt aaagtctgca tacgttctct atcactgata gggagtaaac tcttcatacg
      541 ttctctatca ctgataggga gtaaagccaa ctcgaggcta gtctcgtgat cgataaaatt
      601 ttgaattttt gtaatttgtt tttgtaattc tttagtttgt atgtctgttg ctattatgtc
      661 tactattctt tcccctgcac tgtacccccc aatcccccct tttcttttaa aagttaaccg
      721 ataccgtcga gatccgttca ctaatcgaat ggatctgtct ctgtctctct ctccaccttc
      781 ttcttctatt ccttcgggcc tgtcgggtcc cctcggggtt gggaggtggg tctgaaacga
      841 taatggtgaa tatccctgcc ta
//
