LOCUS       sequence-351212-        9450 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human codon optimized tardigrade damage suppressing 
            protein Dsup under a CMV promoter; to provide increased radio 
            tolerance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9450)
  AUTHORS   Westover C, Najjar D, Meydan C, Grigorev K, Veling MT, Chang ML, 
            Chin C, Butler D, Afshin EE, Silver PA, Mason CE
  TITLE     Multi-omics Analysis of Dsup Expressing Human Cells Reveals Open 
            Chromatin Architectural Dynamics Underlying Radioprotection
  JOURNAL   bioRxiv 2020.11.10.373571
REFERENCE   2  (bases 1 to 9450)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2020.11.10.373571"
COMMENT     Sequence Label: Dsup pLVX-Puro
FEATURES             Location/Qualifiers
     source          1..9450
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    680..805
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1302..1535
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1720..1764
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2027..2142
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        2180..2483
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2508..2711
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2662..2682
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2708..2732
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     RBS             3102..3110
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             4147..4173
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             4147..4173
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    join(4199..4773,4774..4776,4777..4785)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4366..4383)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4593..4612
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4805..5404
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4805..4824)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5301..5321
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5418..6006
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5471..5491)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5889..5900)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6213..6846
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6974..6990)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6974..6990)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6987..7009)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6998..7014
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7022..7028,7029..7046,7047..7052))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7067..7088
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7205..7222)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7376..7964)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7456..7475)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8135..8926,8927..8995))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8758..8777
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8996..9100)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    9148..9282
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9185..9204)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9239..9258
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(9450,1..633)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac
       61 acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact
      121 gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa
      181 taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga
      241 gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga
      301 gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc
      361 tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc
      421 ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag
      481 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
      541 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
      601 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
      661 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
      721 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
      781 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
      841 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
      901 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
      961 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     1021 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     1081 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     1141 agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     1201 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1261 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1321 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1381 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1441 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1501 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1561 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1621 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1681 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1741 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1801 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     1861 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     1921 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     1981 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccttta aaagaaaagg
     2041 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     2101 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     2161 cagcagagat ccagtttatc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     2221 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     2281 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     2341 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     2401 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
     2461 tattagtcat cgctattacc atggtattag tcatcgctat taccatggtg atgcggtttt
     2521 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     2581 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     2641 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     2701 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
     2761 acctccatag aagacaccga ctctactaga ggatctattt ccggtgaatt catggcctcc
     2821 acccaccaga gcagcaccga gccttccagc accggcaaat ccgaggaaac aaagaaggac
     2881 gccagccaag gttccggcca agattccaag aacgtgacag tcaccaaggg caccggctcc
     2941 agcgccacta gcgccgccat cgtgaagacc ggaggcagcc aaggtaaaga cagctccaca
     3001 accgccggat cctccagcac ccaaggtcag aagttctcca ccacccctac agaccctaag
     3061 accttttcct ccgaccagaa ggaaaagtcc aagtcccccg ctaaggaggt gcctagcggc
     3121 ggagactcca agtcccaagg tgacaccaag agccagagcg acgccaaaag cagcggccag
     3181 agccaaggtc agagcaagga ctccggcaag agctcctccg actccagcaa gagccacagc
     3241 gtgatcggcg ccgtgaagga cgtcgtggcc ggcgctaaag atgtggctgg caaagccgtg
     3301 gaggacgccc cctccattat gcataccgcc gtggacgctg tgaagaacgc cgccaccacc
     3361 gtcaaagacg tggcctccag cgctgccagc acagtggccg agaaagtggt cgacgcctac
     3421 cacagcgtgg tcggcgataa gacagatgac aagaaggagg gcgagcacag cggcgacaaa
     3481 aaggacgaca gcaaggctgg aagcggcagc ggccaaggtg gcgataacaa gaagagcgag
     3541 ggcgagacaa gcggccaagc tgagagctcc agcggcaatg agggcgccgc tcccgctaag
     3601 ggcagaggaa ggggcagacc tcccgctgcc gccaaaggag tggccaaagg cgctgctaag
     3661 ggcgccgctg ccagcaaagg cgccaaatcc ggcgccgaga gctccaaagg aggagaacag
     3721 tccagcggcg acatcgagat ggccgacgcc agcagcaagg gaggaagcga ccagagagac
     3781 tccgctgcta ccgtgggaga aggcggagcc agcggaagcg aaggcggcgc caaaaagggc
     3841 agaggccgtg gcgccggcaa aaaggccgat gctggcgata cctccgccga gcctcccaga
     3901 aggagcagca gactgaccag ctccggaact ggtgctggat ccgcccccgc tgctgctaaa
     3961 ggaggcgcca aaagggccgc cagcagcagc agcacacctt ccaacgccaa gaagcaagct
     4021 actggtggcg ctggcaaagc tgccgctacc aaggccacag ccgccaaatc cgccgccagc
     4081 aaggcccccc aaaatggagc cggcgccaag aagaagggcg gaaaggccgg cggtcgtaag
     4141 aggaaatacc cctacgacgt gcccgactac gcctaatcta gagcggccgc ggatcccgcc
     4201 cctctccctc ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg
     4261 cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga
     4321 aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa
     4381 tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa
     4441 caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct
     4501 gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg
     4561 ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg
     4621 ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca
     4681 catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg
     4741 acgtggtttt cctttgaaaa acacgatgat aagcttgcca caacccacaa ggagacgacc
     4801 ttccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tcccccgggc
     4861 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgaccc
     4921 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
     4981 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc
     5041 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
     5101 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
     5161 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
     5221 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
     5281 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
     5341 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
     5401 ctagacgcgt ctggaacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     5461 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     5521 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     5581 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     5641 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     5701 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     5761 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt
     5821 cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     5881 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     5941 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     6001 ccccgcctgg aattaattct gcagtcgaga cctagaaaaa catggagcaa tcacaagtag
     6061 caatacagca gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt
     6121 gggttttcca gtcacacctc aggtaccttt aagaccaatg acttacaagg cagctgtaga
     6181 tcttagccac tttttaaaag aaaagagggg actggaaggg ctaattcact cccaacgaag
     6241 acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg attagcagaa
     6301 ctacacacca gggccagggg tcagatatcc actgaccttt ggatggtgct acaagctagt
     6361 accagttgag ccagataagg tagaagaggc caataaagga gagaacacca gcttgttaca
     6421 ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtgttagagt ggaggtttga
     6481 cagccgccta gcatttcatc acgtggcccg agagctgcat ccggagtact tcaagaactg
     6541 ctgatatcga gcttgctaca agggactttc cgctggggac tttccaggga ggcgtggcct
     6601 gggcgggact ggggagtggc gagccctcag atcctgcata taagcagctg ctttttgcct
     6661 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     6721 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     6781 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     6841 ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     6901 tatcagagag tgagaggcct tgacattgct agcgtttacc gtcgacctct agctagagct
     6961 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     7021 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     7081 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     7141 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     7201 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     7261 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     7321 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     7381 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     7441 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     7501 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     7561 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     7621 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     7681 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     7741 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     7801 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     7861 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     7921 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     7981 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     8041 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     8101 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     8161 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     8221 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     8281 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     8341 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     8401 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     8461 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     8521 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     8581 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     8641 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     8701 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     8761 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     8821 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     8881 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     8941 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     9001 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     9061 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     9121 cacctgacgt cgacggatcg ggagatcaac ttgtttattg cagcttataa tggttacaaa
     9181 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     9241 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaactggat aactcaagct
     9301 aaccaaaatc atcccaaact tcccacccca taccctatta ccactgccaa ttacctgtgg
     9361 tttcatttac tctaaacctg tgattcctct gaattatttt cattttaaag aaattgtatt
     9421 tgttaaatat gtactacaaa cttagtagtt
//
