LOCUS       sequence-35123-e         943 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 943)
  TITLE     other plasmids
REFERENCE   2  (bases 1 to 943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLenti CMVTRE3G eGFP Puro (w819-1)
FEATURES             Location/Qualifiers
     source          1..943
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    40..92
                     /label=MCS
                     /note="multiple cloning site"
     protein_bind    complement(121..145)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(191..569)
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     primer_bind     complement(258..282)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    327..345
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    363..381
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    399..417
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    435..453
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    471..489
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    507..525
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    543..561
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    596..713
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gctcctcgcc cttgctcacc atggtggcga ccggccggtg gatcccgggc ccgcggtacc
       61 gtcgactgca gaattcgaag cttgagctcg agatccagtc gactgaattg gttcctttaa
      121 agcctgcttt tttgtacaaa cttgttgata tctgcagaat tccaccacac tggactagtc
      181 gacgaaccgg tttacgaggg taggaagtgg tacggaaagt tggtataaga caaaagtgtt
      241 gtggaattgc tccaggcgat ctgacggttc actaaacgag ctctgctttt ataggcgccc
      301 accgtacacg cctaaagctt atacgttctc tatcactgat agggagtaaa ctggatatac
      361 gttctctatc actgataggg agtaaactgt agatacgttc tctatcactg atagggagta
      421 aactggtcat acgttctcta tcactgatag ggagtaaact ccttatacgt tctctatcac
      481 tgatagggag taaagtctgc atacgttctc tatcactgat agggagtaaa ctcttcatac
      541 gttctctatc actgataggg agtaaagcca actcgaggct agtctcgtga tcgataaaat
      601 tttgaatttt tgtaatttgt ttttgtaatt ctttagtttg tatgtctgtt gctattatgt
      661 ctactattct ttcccctgca ctgtaccccc caatcccccc ttttctttta aaagttaacc
      721 gataccgtcg agatccgttc actaatcgaa tggatctgtc tctgtctctc tctccacctt
      781 cttcttctat tccttcgggc ctgtcgggtc ccctcggggt tgggaggtgg gtctgaaacg
      841 ataatggtga atatccctgc ctaactctat tcactataga aagtacagca aaaactattc
      901 ttaaacctac caagcctcct actatcatta tgaataattt tat
//
