LOCUS       sequence-35124-e         851 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Murakoshi H, Wang H, Yasuda R
  TITLE     Local, persistent activation of Rho GTPases during plasticity of 
            single dendritic spines.
  JOURNAL   Nature. 2011 Apr 7;472(7341):100-4. doi: 10.1038/nature09823. Epub 
            2011 Mar 20.
  PUBMED    21423166
REFERENCE   2  (bases 1 to 851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature09823"; journalName: "Nature"; date: "2011-04-7- 7"; 
            volume: "472"; issue: "7341"; pages: "100-4"
COMMENT     Sequence Label: mCherry-Pak3(60-113)/S74A/F84A-mCherry-C1
FEATURES             Location/Qualifiers
     source          1..851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             144..851
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(289..307)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     557..576
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     751..774
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 agatttctct ccctgcagat tttgaacaca caattcatgt cggtgctgat gctgtcacag
       61 gggagtttac gggaatacca gagcagtggg cccgcttgct tcagacatca aatatcacta
      121 agttggagca gaagggatcc ggaatggtga gcaagggcga ggaggacaac atggccatca
      181 tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg
      241 agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg
      301 tgaccaaggg cggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg
      361 gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc
      421 ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga
      481 cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca
      541 acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg
      601 agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga
      661 aggacggcgg ccactacgac gccgaggtca agaccaccta caaggccaag aagcccgtgc
      721 agctgcccgg cgcctacaac gtcaacatca agctggacat cacctcccac aacgaggact
      781 acaccatcgt ggaacagtac gagcgcgccg agggccgcca ctccaccggc ggcatggacg
      841 agctgtacaa g
//
