LOCUS       sequence-351254-        4719 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning 
            ToolKit..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4719)
  AUTHORS   Mozsik L, Pohl C, Meyer V, Bovenberg RAL, Nygard Y, Driessen AJM
  TITLE     Modular Synthetic Biology Toolkit for Filamentous Fungi.
  JOURNAL   ACS Synth Biol. 2021 Nov 2. doi: 10.1021/acssynbio.1c00260.
  PUBMED    34726388
REFERENCE   2  (bases 1 to 4719)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2021 Nov 2. doi: 10.1021/acssynbio.1c00260."
COMMENT     Sequence Label: pFTK053
FEATURES             Location/Qualifiers
     source          1..4719
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(24..42)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        110..214
                     /gene="bla"
                     /label=AmpR promoter
     CDS             436..1227
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1398..1986
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1887..1906
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2140..2157
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtggctcttc agtggacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
       61 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
      121 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
      181 ataaatgctt caataatatt gaaaaaggaa gagtatgcgc tcacgcaact ggtccagaac
      241 cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg
      301 tttttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
      361 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
      421 acaaagttaa acatcatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
      481 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
      541 tccgcagtgg atggcggcct gaagccacac agcgatattg atttgctggt tacggtgacc
      601 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
      661 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
      721 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
      781 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
      841 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
      901 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
      961 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     1021 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     1081 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     1141 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccatta cgtaaaaggc
     1201 gagatcacca aggtagtcgg caaataactg tcagaccaag tttactcata tatactttag
     1261 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     1321 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     1381 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     1441 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     1501 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     1561 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     1621 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     1681 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     1741 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     1801 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     1861 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     1921 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     1981 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     2041 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     2101 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     2161 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatca
     2221 ctctgtggtc tcaaatgatg gcagatacgc gccgacgcca gaatcatagc tgcgatccct
     2281 gtcgcaaggg caagcgacgc tgtgatgccc cggaaaatag aaacgaggcc aatgaaaacg
     2341 gctgggtttc gtgttcaaat tgcaagcgtt ggaacaagga ttgtaccttc aattggctct
     2401 catcccaacg ctccaaggca aaaggggctg cacctagagc gagaacaaag aaagccagga
     2461 ccgcaacaac caccagtgaa ccatcaactt cagctgcaac aatccctaca ccggaaagtg
     2521 acaatcacga tgcgcctcca gtcataaact ctcacgacgc gctcccgagc tggactcagg
     2581 ggctactctc ccaccccggc gaccttttcg atttcagcca ctctgctatt cccgcaaatg
     2641 cagaagatgc ggccaacgtg cagtcagacg caccttttcc gtgggatcta gccatccccg
     2701 gtgatttcag catgggccaa cagctcgaga aacctctcag tccgctcagt tttcaagcag
     2761 tccttcttcc gccccatagc ccgaacacgg atgacctcat tcgcgagctg gaagagcaga
     2821 ctacggatcc ggactcggtt actgatacta atagtgtaca acaggtcgct caagatggat
     2881 cgctatggtc tgatcggcag tcgccgctac tgcctgagaa cagtctgtgc atggcctcag
     2941 acagcacagc acggcgatat gcccgttcca caatgacgaa gaatctgatg cgaatctacc
     3001 acgatagtat ggagaatgca ctgtcctgct ggctgacaga gcacaattgt ccatactccg
     3061 accagatcag ctacctgccg cccaagcagc gggcggaatg gggcccgaac tggtcaaaca
     3121 ggatgtgcat ccgggtgtgc cggctagatc gcgtatctac ctcattacgc gggcgcgccc
     3181 tgagtgcgga agaggacaaa gccgcagccc gagccctgca tctggcgatc gtagcttttg
     3241 cgtcgcaatg gacgcagcat gcgcagaggg gggctgggct aaatgttcct gcagacatag
     3301 ccgccgatga gaggtccatc cggaggaacg cctggaatga agcacgccat gccttgcagc
     3361 acacgacagg gattccatca ttccgggtta tatttgcgaa tatcatcttt tctctcacgc
     3421 agagtgtgct ggatgatgat gagcagcacg gtatgggtgc acgtctagac aagctactcg
     3481 aaaatgacgg tgcgcccgtg ttcctggaaa ccgcgaaccg tcagctttat acattccgac
     3541 ataagtttgc acgaatgcaa cgccgcggta aggctttcaa caggctcccg ggaggatctg
     3601 tcgcatcgac attcgccggt attttcgaga caccgacgcc gtcgtctgaa agcccacagc
     3661 ttgacccggt tgtggccagt gaggagcatc gcagtacatt aagccttatg ttctggctag
     3721 ggatcatgtt cgatacacta agcgctgcaa tgtaccagcg accactcgtg gtgtcagatg
     3781 aggatagcca gatatcatcg gcatctccac caaggcgcgg cgctgaaacg ccgatcaacc
     3841 tagactgctg ggagcccccg agacaggtcc cgagcaatca agaaaagagc gacgtatggg
     3901 gcgacctctt cctccgcacc tcggactctc tcccagatca cgaatcccac acacaaatct
     3961 ctcagccagc ggctcgatgg ccctgcacct acgaacaggc cgccgccgct ctctcctctg
     4021 caacgcccgt caaagtcctc ctctaccgcc gcgtcacgca gctccaaacc ctcctctatc
     4081 gcggcgccag ccctgcccgc cttgaagcgg ccatccagag aacgctctac gtttataatc
     4141 actggacagc gaagtaccaa ccatttatgc aggactgcgt tgctaaccac gagctcctcc
     4201 cttcgcgcat ccagtcttgg tacgtcattc tagacggtca ctggcatcta gccgcgatgt
     4261 tgctagcgga cgttttggag agcatcgacc gcgattcgta ctctgatatc aaccacatcg
     4321 accttgtaac aaagctaagg ctcgataatg cactagcagt tagtgccctt gcgcgctctt
     4381 cactccgagg ccaggagctg gacccgggca aagcatctcc gatgtatcgc catttccatg
     4441 attctctgac cgaggtggca ttcctggtag aaccgtggac cgtcgttctt attcactcgt
     4501 ttgccaaagc tgcgtatatc ttgctggact gtttagatct ggacggccaa ggaaatgcac
     4561 tagcggggta cctgcagctg cggcaaaatt gcaactactg cattcgggcg ctgcaatttc
     4621 tgggcaggaa gtcggatatg gcggcgctgg ttgcgaagga tttagagaga ggtttgaatg
     4681 ggaaagttga cagctttttg taggctttga gaccacgaa
//
