LOCUS       sequence-351257-        5009 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning 
            ToolKit..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5009)
  AUTHORS   Mozsik L, Pohl C, Meyer V, Bovenberg RAL, Nygard Y, Driessen AJM
  TITLE     Modular Synthetic Biology Toolkit for Filamentous Fungi.
  JOURNAL   ACS Synth Biol. 2021 Nov 2. doi: 10.1021/acssynbio.1c00260.
  PUBMED    34726388
REFERENCE   2  (bases 1 to 5009)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2021 Nov 2. doi: 10.1021/acssynbio.1c00260."
COMMENT     Sequence Label: pFTK054
FEATURES             Location/Qualifiers
     source          1..5009
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(24..42)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        110..214
                     /gene="bla"
                     /label=AmpR promoter
     CDS             436..1227
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1398..1986
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1887..1906
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2140..2157
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtggctcttc agtggacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
       61 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
      121 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
      181 ataaatgctt caataatatt gaaaaaggaa gagtatgcgc tcacgcaact ggtccagaac
      241 cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg
      301 tttttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
      361 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
      421 acaaagttaa acatcatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
      481 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
      541 tccgcagtgg atggcggcct gaagccacac agcgatattg atttgctggt tacggtgacc
      601 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
      661 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
      721 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
      781 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
      841 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
      901 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
      961 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     1021 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     1081 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     1141 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccatta cgtaaaaggc
     1201 gagatcacca aggtagtcgg caaataactg tcagaccaag tttactcata tatactttag
     1261 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     1321 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     1381 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     1441 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     1501 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     1561 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     1621 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     1681 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     1741 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     1801 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     1861 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     1921 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     1981 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     2041 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     2101 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     2161 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatca
     2221 ctctgtggtc tcaaatgaac actattccag cgcgtcacgt tggagatgtg gcagctcgtg
     2281 acccactccc cctgccacat ataagttcca gtgtggccag tggcatgaaa cgatcgtttg
     2341 ccactatggc catgctctat aatgatacgg gcaatagcaa tgatgtcgga gcgcatgcgc
     2401 gacgcccacc gcgcactctg agtaacagcc gcagcacatc ggctcaccgg gtcccattgg
     2461 gtagctggag cgccccgaac tcgccaccgc gacgcgcact cccacaccac cccataacag
     2521 cttcgttcga cccggacgcc tcgatcgtga ttgccggaat ccgcggtgct ggaaagtcca
     2581 ctctcgccat aatggcttcg acggcgatga agcgcaagat cgttgacctc gaatcggaat
     2641 ttcaccacct gactggcctg tcctccagca gctacaagaa aacacacggc ccggtggact
     2701 atggtcgccg ccagatagca atcctgcaga acatccttaa cctgcaccga acgcgcgcca
     2761 ttctggtatg ttcctggttg gagcgcgatg tgcaggcaat gcttcaggat ttttcggtca
     2821 gcaacccggt gatctatgtc ctcagggatg ccaaagccat tgaggcccac ctgaaaggtt
     2881 atgacaagtc gaaggtgggt acgttgctgg atgcgacctc gaccgtcctg aggcgatgca
     2941 cgcgatttga gttcttcaat gtctccgaag aaaatctgga tactcattcc gccagcacgt
     3001 cccccccagc ggttccagat cagaggcata ctgctcccta cctgactctt aagcgcgcag
     3061 agcgtcactt tcttaagttc ctgtccttga ttttgcccaa aggaaccatc ccgttcgtag
     3121 aatccgcctt cccactggcg tcggttccgg tggagcaacg ccgcttcacc tatgcgctgg
     3181 cgcttcccgt gtcggcactc ctggacaagg gagtcgacat tcaggagctt gacgtaggcg
     3241 tcgacgcaat cgaaattatc gtagacgatc tggccacaag cgaatccggc ccaactagcc
     3301 ccctcggcct cgccccgcac cgagcaagcg aaatctccag ggtggttggc gagatccgca
     3361 gggataccgt catacccatc atcctccacg tggttttccc ggagagggcc ctgtacgaag
     3421 aggccctgtt ggccctgtac atgacctacc tgaaccacgc cttgcgtctc gcgcccgatt
     3481 accttacagt ggacctcgga ctggatagcg gcctgctggg tcagttgacg acagtgcagg
     3541 gcacaactaa agtgattggt aacaagcagc tggctgaagt gaatagccct cgctggggcg
     3601 acccatcgtg gttgcaggcc tacgaaaagg cccaaaatac tggctgtgac cttgtgcggt
     3661 tgacacgacc cgcctcgaac ccgcgtgaca atactgacat ccgtcagttc catgttgccg
     3721 tggaggctgt gggtggccca cgactgccct tcatcgccta taatactggt aggctcggcc
     3781 gaaccagtat gtgcttcaac gaaatcctga cgccagtcac tcccgtcccc accaaggagg
     3841 atgccattgg tctgcgcaat ccggcacacc gttacctgca gccaccgttg actgcactgg
     3901 aagcaaccca ggccctgtac agcgcgtttg ttcacgaccc aatgaagctg tacgttttcg
     3961 gagctaatgt tggatacagc ctgagccccg ctatgcacaa tgccgcgttg aaagcctgcg
     4021 gaatcccgca tcattacaag cccctgagta ctgctaacat aggcaccctt cgtgaggtga
     4081 tctccgaccc gcagttcgcg ggagctagtg taggtctgcc ctttaaggtg gaaatcatta
     4141 gcctgactca ctcgctgagc cgtcacgcta aagccattgg cgcggtgaat acgttgattc
     4201 ctgtgaggca tcttacagca gatggaggca tccccgacga ggtgtccatg tttaataaca
     4261 tcagccaggc aggtgctgtg agggcattgt acggcgagaa taccgattgg atcggaatcc
     4321 gtgcctgtct gcgtcgaggt ttgagtcctg caaatgcagt ccgctccaca tccactggat
     4381 tggtgatcgg agcaggcggc atggcccgag cagcggtgta cgcaatgctc cagctcggag
     4441 tcaagaagat cctcattttc aaccgcacct tcgctaacgc cgagaagctc gtgctgcatt
     4501 tcgagaatct gttggtcagg gatgccctgc ccctgttgtc gacaggccct cgcagccacg
     4561 ataacacgtg ttttcacatc atacggtcga gggatgatcc gctcccagag aacttcaaga
     4621 accctaccat gatagtgtcg tgcatcccaa cgcacactgt agataatacg cccgatcccg
     4681 agttcaccgt gccattgcac tggttggaca acccgaccgg cggaatcgta ctggagctgg
     4741 actacaagtg cttgacaagt ccactgttgg agcagacgcg gcgtgaggct catcgcggat
     4801 gggttgcgat ggatggcttg gacctgctcc ctgaacaagg atttgcgcag tttgaattgt
     4861 tcaccggccg acgtgcaccg cgtcgcctga tgcgccgcga ggtcctgcga gcatatccgg
     4921 acgaccaggc caagagtcat actgcacagc tgcaacctcg tctcaatgga atagcaacgc
     4981 agatttcgta aaagctttga gaccacgaa
//
