LOCUS       sequence-35126-e         715 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 715)
  AUTHORS   Chen G, Gulbranson DR, Hou Z, Bolin JM, Ruotti V, Probasco MD, 
            Smuga-Otto K, Howden SE, Diol NR, Propson NE, Wagner R, Lee GO, 
            Antosiewicz-Bourget J, Teng JM, Thomson JA
  TITLE     Chemically defined conditions for human iPSC derivation and culture.
  JOURNAL   Nat Methods. 2011 May;8(5):424-9. doi: 10.1038/nmeth.1593. Epub 2011
            Apr 10.
  PUBMED    21478862
REFERENCE   2  (bases 1 to 715)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1593"; journalName: "Nat Methods"; date: "2011-05"; 
            volume: "8"; issue: "5"; pages: "424-9"
COMMENT     Sequence Label: pET-3c-rhVTN_FL
FEATURES             Location/Qualifiers
     source          1..715
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             102..124
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa ttaatacgac
       61 tcactatagg gagaccacaa cggtttccct ctagaaataa ttttgtttaa ctttaagaag
      121 gagatataca tatggaccaa gagtcatgca agggccgctg cactgagggc ttcaacgtgg
      181 acaagaagtg ccagtgtgac gagctctgct cttactacca gagctgctgc acagactata
      241 cggctgagtg caagccccaa gtgactcgcg gggatgtgtt cactatgccg gaggatgagt
      301 acacggtcta tgacgatggc gaggagaaaa acaatgccac tgtccatgaa caggtggggg
      361 gcccctccct gacctctgac ctccaggccc agtccaaagg gaatcctgag cagacacctg
      421 ttctgaaacc tgaggaagag gcccctgcgc ctgaggtggg cgcctctaag cctgagggga
      481 tagactcaag gcctgagacc cttcatccag ggagacctca gcccccagca gaggaggagc
      541 tgtgcagtgg gaagcccttc gacgccttca ccgacctcaa gaacggttcc ctctttgcct
      601 tccgagggca gtactgctat gaactggacg aaaaggcagt gaggcctggg taccccaagc
      661 tcatccgaga tgtctggggc atcgagggcc ccatcgatgc cgccttcacc cgcat
//
