LOCUS       sequence-351264-       10438 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  construct expresses the reverse tet-responsive transactivator 3 
            (rtTA3) together with Luciferase (Luc) for bioluminescence in vivo 
            imaging, and mTaqBFP for enriching transgenic cells by flow 
            cytometry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10438)
  AUTHORS   Liu WH, Mrozek-Gorska P, Wirth AK, Herold T, Schwarzkopf L, Pich D, 
            Volse K, Melo-Narvaez MC, Carlet M, Hammerschmidt W, Jeremias I
  TITLE     Inducible transgene expression in PDX models in vivo identifies KLF4
            as a therapeutic target for B-ALL.
  JOURNAL   Biomark Res. 2020 Sep 16;8:46. doi: 10.1186/s40364-020-00226-z. 
            eCollection 2020.
  PUBMED    32944247
REFERENCE   2  (bases 1 to 10438)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biomark 
            Res."; date: "2020-09-16"; volume: "8:46. doi"; pages: " 
            10.1186/s40364-020-00226-z. eCollection 2020"
COMMENT     Sequence Label: pRRL-SFFV-rtTA3-IRES-Luc-P2A-mtagBFP
FEATURES             Location/Qualifiers
     source          1..10438
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1806..1923
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1961..2367
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             join(2388..3005,3006..3011,3012..3047,3048..3050,3051..3086,
                     3087..3089,3090..3125,3126..3134)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    3151..3718
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3168..3720
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3312..3329)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3539..3558
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3732..5381
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     CDS             5403..5459
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5466..6167
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     misc_feature    6187..6775
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6240..6260)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6658..6669)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6982..7215
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7287..7408
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7324..7343)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7378..7397
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7427..7562
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7582..7601)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7583..7601)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7611..7628)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7611..7627)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7620..7642)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7769..8224
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7856..7875)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8066..8087
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8250..8354
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8355..8423,8424..9215)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8573..8592)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9386..9974
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9875..9894
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10128..10145
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10262..10283
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10298..10303,10304..10321,10322..10328)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10336..10352
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10341..10363
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10360..10376
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10360..10376
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10395..10415
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10397..10415
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     1741 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcgg
     1801 ttaactttta aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac
     1861 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat
     1921 tttatcgatc acgagactag cctcgatcga gctagctgca gtaacgccat tttgcaaggc
     1981 atggaaaaat accaaaccaa gaatagagaa gttcagatca agggcgggta catgaaaata
     2041 gctaacgttg ggccaaacag gatatctgcg gtgagcagtt tcggccccgg cccggggcca
     2101 agaacagatg gtcaccgcag tttcggcccc ggcccgaggc caagaacaga tggtccccag
     2161 atatggccca accctcagca gtttcttaag acccatcaga tgtttccagg ctcccccaag
     2221 gacctgaaat gaccctgcgc cttatttgaa ttaaccaatc agcctgcttc tcgcttctgt
     2281 tcgcgcgctt ctgcttcccg agctctataa aagagctcac aacccctcac tcggcgcgcc
     2341 agtcctccga cagactgagt cggccggtgg atctaccgga gctagggatg tctagactgg
     2401 acaagagcaa agtcataaac ggagctctgg aattactcaa tggtgtcggt atcgaaggcc
     2461 tgacgacaag gaaactcgct caaaagctgg gagttgagca gcctaccctg tactggcacg
     2521 tgaagaacaa gcgggccctg ctcgatgccc tgccaatcga gatgctggac aggcatcata
     2581 cccacttctg ccccctggaa ggcgagtcat ggcaagactt tctgcggaac aacgccaagt
     2641 cataccgctg tgctctcctc tcacatcgcg acggggctaa agtgcatctc ggcacccgcc
     2701 caacagagaa acagtacgaa accctggaaa atcagctcgc gttcctgtgt cagcaaggct
     2761 tctccctgga gaacgcactg tacgctctgt ccgccgtggg ccactttaca ctgggctgcg
     2821 tattggagga acaggagcat caagtagcaa aagaggaaag agagacacct accaccgatt
     2881 ctatgccccc acttctgaga caagcaattg agctgttcga ccggcaggga gccgaacctg
     2941 ccttcctttt cggcctggaa ctaatcatat gtggcctgga gaaacagcta aagtgcgaaa
     3001 gcggcgggcc gaccgacgcc cttgacgatt ttgacttaga catgctccca gccgatgccc
     3061 ttgacgactt tgaccttgat atgctgcctg ctgacgctct tgacgatttt gaccttgaca
     3121 tgctccccgg gtaagtcgag gttaacgaat tccgcccccc ccccctaacg ttactggccg
     3181 aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc
     3241 gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag
     3301 gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt
     3361 tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa
     3421 ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc
     3481 aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg
     3541 gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat
     3601 gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa
     3661 acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataata
     3721 tggccacaac catggaagat gccaaaaaca ttaagaaggg cccagcgcca ttctacccac
     3781 tcgaagacgg gaccgccggc gagcagctgc acaaagccat gaagcgctac gccctggtgc
     3841 ccggcaccat cgcctttacc gacgcacata tcgaggtgga cattacctac gccgagtact
     3901 tcgagatgag cgttcggctg gcagaagcta tgaagcgcta tgggctgaat acaaaccatc
     3961 ggatcgtggt gtgcagcgag aatagcttgc agttcttcat gcccgtgttg ggtgccctgt
     4021 tcatcggtgt ggctgtggcc ccagctaacg acatctacaa cgagcgcgag ctgctgaaca
     4081 gcatgggcat cagccagccc accgtcgtat tcgtgagcaa gaaagggctg caaaagatcc
     4141 tcaacgtgca aaagaagcta ccgatcatac aaaagatcat catcatggat agcaagaccg
     4201 actaccaggg cttccaaagc atgtacacct tcgtgacttc ccatttgcca cccggcttca
     4261 acgagtacga cttcgtgccc gagagcttcg accgggacaa aaccatcgcc ctgatcatga
     4321 acagtagtgg cagtaccgga ttgcccaagg gcgtagccct accgcaccgc accgcttgtg
     4381 tccgattcag tcatgcccgc gaccccatct tcggcaacca gatcatcccc gacaccgcta
     4441 tcctcagcgt ggtgccattt caccacggct tcggcatgtt caccacgctg ggctacttga
     4501 tctgcggctt tcgggtcgtg ctcatgtacc gcttcgagga ggagctattc ttgcgcagct
     4561 tgcaagacta taagattcaa tctgccctgc tggtgcccac actatttagc ttcttcgcta
     4621 agagcactct catcgacaag tacgacctaa gcaacttgca cgagatcgcc agcggcgggg
     4681 cgccgctcag caaggaggta ggtgaggccg tggccaaacg cttccaccta ccaggcatcc
     4741 gccagggcta cggcctgaca gaaacaacca gcgccattct gatcaccccc gaaggggacg
     4801 acaagcctgg cgcagtaggc aaggtggtgc ccttcttcga ggctaaggtg gtggacttgg
     4861 acaccggtaa gacactgggt gtgaaccagc gcggcgagct gtgcgtccgt ggccccatga
     4921 tcatgagcgg ctacgttaac aaccccgagg ctacaaacgc tctcatcgac aaggacggct
     4981 ggctgcacag cggcgacatc gcctactggg acgaggacga gcacttcttc atcgtggacc
     5041 ggctgaagag cctgatcaaa tacaagggct accaggtagc cccagccgaa ctggagagca
     5101 tcctgctgca acaccccaac atcttcgacg ccggggtcgc cggcctgccc gacgacgatg
     5161 ccggcgagct gcccgccgca gtcgtcgtgc tggaacacgg taaaaccatg accgagaagg
     5221 agatcgtgga ctatgtggcc agccaggtta caaccgccaa gaagctgcgc ggtggtgttg
     5281 tgttcgtgga cgaggtgcct aaaggactga ccggcaagtt ggacgcccgc aagatccgcg
     5341 agattctcat taaggccaag aagggcggca agatcgccgt gctcgagtcc ggaggaagcg
     5401 gagctactaa cttcagcctg ctgaagcagg ctggagacgt ggaggagaac cctggaccta
     5461 tgcatatgag cgagctgatt aaggagaaca tgcacatgaa gctgtacatg gagggcaccg
     5521 tggacaacca tcacttcaag tgcacatccg agggcgaagg caagccctac gagggcaccc
     5581 agaccatgag aatcaaggtg gtcgagggcg gccctctccc cttcgccttc gacatcctgg
     5641 ctactagctt cctctacggc agcaagacct tcatcaacca cacccagggc atccccgact
     5701 tcttcaagca gtccttccct gagggcttca catgggagag agtcaccaca tacgaagacg
     5761 ggggcgtgct gaccgctacc caggacacca gcctccagga cggctgcctc atctacaacg
     5821 tcaagatcag aggggtgaac ttcacatcca acggccctgt gatgcagaag aaaacactcg
     5881 gctgggaggc cttcaccgag acgctgtacc ccgctgacgg cggcctggaa ggcagaaacg
     5941 acatggccct gaagctcgtg ggcgggagcc atctgatcgc aaacatcaag accacatata
     6001 gatccaagaa acccgctaag aacctcaaga tgcctggcgt ctactatgtg gactacagac
     6061 tggaaagaat caaggaggcc aacaacgaga cctacgtcga gcagcacgag gtggcagtgg
     6121 ccagatactg cgacctccct agcaaactgg ggcacaagct taattaagtc gacacgcgtc
     6181 tggaacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     6241 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     6301 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     6361 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     6421 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     6481 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     6541 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg
     6601 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     6661 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     6721 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgga
     6781 attaattctg cagtcgagac ctagaaaaac atggagcaat cacaagtagc aatacagcag
     6841 ctaccaatgc tgattgtgcc tggctagaag cacaagagga ggaggaggtg ggttttccag
     6901 tcacacctca ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact
     6961 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc
     7021 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     7081 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     7141 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     7201 ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa
     7261 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat
     7321 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     7381 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac
     7441 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     7501 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     7561 cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc
     7621 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     7681 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     7741 cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg
     7801 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     7861 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     7921 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     7981 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     8041 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     8101 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     8161 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
     8221 atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     8281 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     8341 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     8401 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     8461 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     8521 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     8581 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     8641 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     8701 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     8761 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     8821 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     8881 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     8941 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     9001 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     9061 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     9121 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     9181 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     9241 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     9301 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     9361 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     9421 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     9481 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     9541 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     9601 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     9661 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     9721 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     9781 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     9841 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     9901 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     9961 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
    10021 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
    10081 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
    10141 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
    10201 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
    10261 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
    10321 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
    10381 attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagc
//
