LOCUS       sequence-351282-       10453 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes SARS-CoV-2 B.1.1.529 Spike Protein lacking 18 C-terminal 
            amino acids for pseudovirus production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10453)
  AUTHORS   Garcia-Beltran WF, St Denis KJ, Hoelzemer A, Lam EC, Nitido AD, 
            Sheehan ML, Berrios C, Ofoman O, Chang CC, Hauser BM, Feldman J, 
            Roederer AL, Gregory DJ, Poznansky MC, Schmidt AG, Iafrate AJ, 
            Naranbhai V, Balazs AB
  TITLE     mRNA-based COVID-19 vaccine boosters induce neutralizing immunity 
            against SARS-CoV-2 Omicron variant.
  JOURNAL   Cell. 2022 Jan 6. pii: S0092-8674(21)01496-3. doi: 
            10.1016/j.cell.2021.12.033.
  PUBMED    34995482
REFERENCE   2  (bases 1 to 10453)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2022 
            Jan 6. pii: S0092-8674(21)01496-3. doi: 10.1016/j.cell.2021.12.033."
COMMENT     Sequence Label: pTwist-SARS-CoV-2 Delta-18 B.1.1.529
FEATURES             Location/Qualifiers
     source          1..10453
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    3923..4511
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3976..3996)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4394..4405)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4564..4685
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4575..4594)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4629..4648
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      4800..4846
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      4876..5012
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    5057..5128
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     promoter        5173..5369
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5220..5355
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5383..6177
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5437..5456)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6047..6066
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6188..6412
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(6608..7196)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6688..6707)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(7942..8010,8011..8802)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8160..8179)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      8815..8951
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    9103..9174
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(9179..9225)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    9243..9291
                     /note="synthetic polyadenylation signal"
     enhancer        9372..9675
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9677..9879
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9830..9850
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          join(10013..10453,1..35)
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
ORIGIN
        1 taatcatgtt catacctctt atcttcctcc cacagctcct gggcaacgtg ctggtctgtg
       61 tgctggccca tcactttggc aaaggcggcc gccatgtttg tgttcctcgt tctcctgccc
      121 ctcgtttctt cccaatgcgt gaacctcaca actagaacgc agctcccacc cgcttacacg
      181 aactctttta ctcgtggagt atactatccc gataaagtct ttaggtccag tgtccttcat
      241 tctactcaag accttttcct gccattcttc tcaaatgtca cctggttcca tgttatctct
      301 ggcaccaacg gaactaaacg atttgataat cccgtcttgc catttaacga tggcgtatac
      361 tttgcctcaa tagagaagtc caacattatc cggggatgga tatttggcac caccctggac
      421 tccaagacac agtctctcct cattgtgaac aacgccacca atgtcgtcat caaagtctgt
      481 gaatttcaat tttgtaacga tccctttctc gatcataaga ataacaagtc ttggatggag
      541 tcagagttcc gcgtttacag ttccgctaac aactgtactt ttgagtacgt ttcccaacca
      601 tttctcatgg atcttgaagg gaagcaaggc aacttcaaga atctccgcga atttgtgttt
      661 aagaatatag acggctactt caaaatttat agcaagcata ccccgataat tgttcgggaa
      721 cccgaagatc tccctcaagg cttctccgcg ttggaacccc tcgtggactt gccgattggc
      781 atcaacatca cccggttcca aacccttctt gcccttcatc gaagctacct cacgcctggc
      841 gacagtagta gtggttggac agcgggcgca gcagcctact atgtgggata tttgcaacca
      901 cgcacctttc ttctcaaata taacgaaaac ggaacaatta ccgatgccgt agactgtgcc
      961 ctcgatcccc tgtccgaaac taaatgcaca ctcaagtcct tcacagttga gaaaggcata
     1021 taccaaacca gtaacttccg cgtccaaccc accgaatcca ttgttcgatt ccctaacatc
     1081 accaatttgt gtcctttcga tgaagtattt aatgccactc gatttgcatc agtttatgcc
     1141 tggaatcgca agcgaatatc aaattgtgtt gcagattata gtgtcctgta taatctcgca
     1201 ccattcttca cttttaagtg ttatggggtc agccctacga aattgaatga cttgtgtttt
     1261 accaacgttt acgcagattc tttcgtcata cgcggcgacg aagtccgaca gattgcaccg
     1321 gggcaaaccg gaaatatagc agactataat tataagttgc cggatgactt caccggctgc
     1381 gttatagcat ggaactccaa taagctcgat tcaaaagtca gcggcaacta taattatctt
     1441 taccgcctgt ttcgcaagtc aaatctgaag ccctttgaac gtgacatctc caccgaaatt
     1501 tatcaagctg gcaacaaacc atgtaatgga gtggcagggt ttaactgtta ttttcccctt
     1561 cgatcatact catttcgacc aacatatggg gtgggccatc aaccatatcg ggtggtagtc
     1621 ttgagctttg agctgctcca cgcaccggct acagtttgcg gaccgaagaa aagcactaac
     1681 ctggtgaaga ataaatgtgt gaacttcaac tttaatgggc tcaaaggaac aggggtgctg
     1741 acagaatcaa acaagaaatt tctccctttt caacaatttg gtagagacat tgccgatacc
     1801 acagatgccg tccgcgatcc gcaaacactg gaaatcctcg atataacacc ctgtagcttc
     1861 ggcggggtca gcgtgataac tcccggaacg aacacatcca accaagtggc tgtcctgtat
     1921 caaggtgtca actgtaccga agtccctgtg gcgatacacg ccgaccaact gacacccacc
     1981 tggagagtct actcaacagg ttccaatgtc tttcaaacta gagcgggttg tctcattggc
     2041 gctgagtatg tcaataattc atacgaatgt gatattccta ttggggcagg aatctgtgcg
     2101 tcttatcaaa ctcaaacgaa atcacaccgg cgcgctcgct cagtagccag ccagtccata
     2161 atcgcctata ccatgagtct cggggctgag aactccgtgg cttattcaaa caattccatc
     2221 gcaatcccaa ctaactttac cattagcgtt accacggaaa tcttgcccgt cagcatgact
     2281 aagacgagcg tagactgtac gatgtatatc tgtggggatt ccaccgagtg tagcaacctc
     2341 ttgcttcaat acgggtcttt ctgtactcaa ctcaaacgag cattgaccgg aattgcggtc
     2401 gaacaagaca agaataccca agaggtgttc gcacaagtaa agcaaatata caaaacgccg
     2461 ccaatcaaat attttggcgg gtttaatttt agccaaatac tgccagaccc atctaagcct
     2521 tcaaaacgca gctttatcga ggatctgctg ttcaacaaag ttacgttggc tgacgcgggt
     2581 ttcattaagc agtatgggga ttgtttggga gacattgcgg cccgcgacct tatctgtgct
     2641 caaaaattca aggggttgac agttctgccc cctctgctca ctgatgagat gattgcacag
     2701 tatacatcag cattgttggc aggaaccatt acatccggct ggacttttgg cgcgggtgcg
     2761 gcccttcaaa tacctttcgc tatgcaaatg gcttacagat tcaatgggat tggtgtcacg
     2821 caaaatgtat tgtatgaaaa tcagaagctg atcgctaatc aatttaacag cgcgattggc
     2881 aagatccaag attcactgtc aagcacagcg tccgccctcg gcaaactcca agatgttgtg
     2941 aatcacaacg cccaggccct gaataccctc gtgaagcaac tgagttctaa attcggcgca
     3001 atctccagcg tcctcaacga catattctcc agacttgata aggtggaagc cgaggtgcaa
     3061 attgatcggc tcatcacagg caggctccag tcactccaaa cttatgtgac acaacagttg
     3121 atccgggctg ctgagatacg agcaagcgcc aacctggccg ccactaaaat gtcagagtgc
     3181 gtgctcgggc agtccaaacg ggttgacttt tgtggcaagg gctatcactt gatgtccttc
     3241 ccccaatccg ccccacatgg agtggtcttt ctccacgtca cttatgtccc tgctcaagag
     3301 aagaacttta cgacagctcc agcaatctgt cacgatggca aagcgcactt tcccagggaa
     3361 ggcgttttcg tcagtaatgg cacgcattgg tttgtgaccc aaaggaattt ctatgaaccc
     3421 caaatcataa ccactgacaa tacatttgtc tcaggcaact gtgatgtggt cattgggatc
     3481 gtgaacaaca cggtatatga tcccctgcaa cccgagttgg atagcttcaa agaggaattg
     3541 gataaatact ttaagaatca tacttcaccc gatgttgatc tcggggacat atcaggaata
     3601 aatgcaagtg tcgtcaacat tcagaaagag attgatcgcc ttaatgaggt cgccaagaac
     3661 ttgaatgaat ctttgatcga cttgcaagaa ctgggcaaat acgaacaata tattaaatgg
     3721 ccctggtaca tttggttggg ctttatcgcc ggacttatag ctatcgttat ggtcacaatt
     3781 atgttgtgct gtatgactag ctgttgttcc tgcttgaaag gatgttgcag ttgcggatct
     3841 tgttgtaaat aaaggatcct ctagaagttg tctcctcctg cactgactga ctgatacaat
     3901 cgatttctgg atccgcaggc ctaatcaacc tctggattac aaaatttgtg aaagattgac
     3961 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     4021 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     4081 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     4141 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     4201 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     4261 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct
     4321 gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     4381 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     4441 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     4501 cgcctccccg cctgtctagc ttgactgact gagatacagc gtaccttcag ctcacagaca
     4561 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
     4621 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
     4681 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg
     4741 ttttttaaag caagtaaaac ctctacaaat gtggtattgg cccatctcta tcggtatcgt
     4801 agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgtgcc cctcgggccg
     4861 gattgctatc taccggcatt ggcgcagaaa aaaatgcctg atgcgacgct gcgcgtctta
     4921 tactcccaca tatgccagat tcagcaacgg atacggcttc cccaacttgc ccacttccat
     4981 acgtgtcctc cttaccagaa atttatcctt aaggtcgtca gctatcctgc aggcgatctc
     5041 tcgatttcga tcaagacatt cctttaatgg tcttttctgg acaccactag gggtcagaag
     5101 tagttcatca aactttcttc cctccctaat ctcattggtt accttgggct atcgaaactt
     5161 aattaagcga tctgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     5221 tcccgcccct aactccgccc agttccgccc attctccgcc ccatcgctga ctaatttttt
     5281 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     5341 gcttttttgg aggcctaggc ttttgcaaag gaggtagcca acatgattga acaagatgga
     5401 ttgcacgcag gttctcccgc cgcttgggtg gagaggctat tcggctatga ctgggcacaa
     5461 cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt
     5521 ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tccaggacga ggcagcgcgg
     5581 ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa
     5641 gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac
     5701 cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt
     5761 gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact
     5821 cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg
     5881 ccagccgaac tgttcgccag gctcaaggcg cggatgcccg acggcgagga tctcgtcgtg
     5941 acccacggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc
     6001 atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt
     6061 gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc
     6121 gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctagtat
     6181 gtaagccctg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     6241 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     6301 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     6361 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggttaattaa
     6421 ccagtcaagt cagctacttg gcgagatcga cttgtctggg tttcgactac gctcagaatt
     6481 gcgtcagtca agttcgatct ggtccttgct attgcacccg ttctccgatt acgagtttca
     6541 tttaaatcat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     6601 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     6661 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     6721 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     6781 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     6841 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     6901 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     6961 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     7021 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     7081 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     7141 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     7201 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     7261 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     7321 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     7381 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcatttaaat
     7441 ttccgaactc tccaaggccc tcgtcggaaa atcttcaaac ctttcgtccg atccatcttg
     7501 caggctacct ctcgaacgaa ctatcgcaag tctcttggcc ggccttgcgc cttggctatt
     7561 gcttggcagc gcctatcgcc aggtattact ccaatcccga atatccgaga tcgggatcac
     7621 ccgagagaag ttcaacctac atcctcaatc ccgatctatc cgagatccga ggaatatcga
     7681 aatcggggcg cgcctggtgt accgagaacg atcctctcag tgcgagtctc gacgatccat
     7741 atcgttgctt ggcagtcagc cagtcggaat ccagcttggg acccaggaag tccaatcgtc
     7801 agatattgta ctcaagcctg gtcacggcag cgtaccgatc tgtttaaacc tagatattga
     7861 tagtctgatc ggtcaacgta taatcgagtc ctagcttttg caaacatcta tcaagagaca
     7921 ggatcagcag gaggctttcg catgagtatt caacatttcc gtgtcgccct tattcccttt
     7981 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     8041 gctgaagatc agttgggtgc gcgagtgggt tacatcgaac tggatctcaa cagcggtaag
     8101 atccttgaga gttttcgccc cgaagaacgc tttccaatga tgagcacttt taaagttctg
     8161 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     8221 cactattctc agaatgactt ggttgagtat tcaccagtca cagaaaagca tcttacggat
     8281 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     8341 aacttacttc tgacaacgat tggaggaccg aaggagctaa ccgctttttt gcacaacatg
     8401 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     8461 gacgagcgtg acaccacgat gcctgtagca atggcaacaa ccttgcgtaa actattaact
     8521 ggcgaactac ttactctagc ttcccggcaa cagttgatag actggatgga ggcggataaa
     8581 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     8641 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     8701 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     8761 cagatcgctg agataggtgc ctcactgatt aagcattggt aaccgattct aggtgcattg
     8821 gcgcagaaaa aaatgcctga tgcgacgctg cgcgtcttat actcccacat atgccagatt
     8881 cagcaacgga tacggcttcc ccaacttgcc cacttccata cgtgtcctcc ttaccagaaa
     8941 tttatcctta agatcccgaa tcgtttaaac tcgactctgg ctctatcgaa tctccgtcgt
     9001 ttcgagctta cgcgaacagc cgtggcgctc atttgctcgt cgggcatcga atctcgtcag
     9061 ctatcgtcag cttacctttt tggcagcgat cgcggctccc gacatcttgg accattagct
     9121 ccacaggtat cttcttccct ctagtggtca taacagcagc ttcagctacc tctcaattca
     9181 aaaaacccct caagacccgt ttagaggccc caaggggtta tgctatcaat cgttgcgtta
     9241 cacacacaaa aaaccaacac acatccatct tcgatggata gcgattttat tatctaactg
     9301 ctgatcgagt gtagccagat ctagtaatca attacggggt cattagttca tagcccatat
     9361 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
     9421 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
     9481 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
     9541 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
     9601 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
     9661 atcgctatta ccatgctgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
     9721 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
     9781 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
     9841 ggtaggcgtg tacggtggga ggtctatata agcagagctg gtttagtgaa ccgtcagatc
     9901 gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggga ccgatccagc
     9961 ctcccctcga agcttacatg tggtaccgag ctcggatcct gagaacttca gggtgagtct
    10021 atgggaccct tgatgttttc tttccccttc ttttctatgg ttaagttcat gtcataggaa
    10081 ggggagaagt aacagggtac acatattgac caaatcaggg taattttgca tttgtaattt
    10141 taaaaaatgc tttcttcttt taatatactt ttttgtttat cttatttcta atactttccc
    10201 taatctcttt ctttcagggc aataatgata caatgtatca tgcctctttg caccattcta
    10261 aagaataaca gtgataattt ctgggttaag gcaatagcaa tatttctgca tataaatatt
    10321 tctgcatata aattgtaact gatgtaagag gtttcatatt gctaatagca gctacaatcc
    10381 agctaccatt ctgcttttat tttatggttg ggataaggct ggattattct gagtccaagc
    10441 taggcccttt tgc
//
