LOCUS       sequence-351283-       10456 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes SARS-CoV-2 B.1.1.529 Spike Protein lacking 18 C-terminal 
            amino acids for pseudovirus production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10456)
  AUTHORS   Garcia-Beltran WF, St Denis KJ, Hoelzemer A, Lam EC, Nitido AD, 
            Sheehan ML, Berrios C, Ofoman O, Chang CC, Hauser BM, Feldman J, 
            Roederer AL, Gregory DJ, Poznansky MC, Schmidt AG, Iafrate AJ, 
            Naranbhai V, Balazs AB
  TITLE     mRNA-based COVID-19 vaccine boosters induce neutralizing immunity 
            against SARS-CoV-2 Omicron variant.
  JOURNAL   Cell. 2022 Jan 6. pii: S0092-8674(21)01496-3. doi: 
            10.1016/j.cell.2021.12.033.
  PUBMED    34995482
REFERENCE   2  (bases 1 to 10456)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2022 
            Jan 6. pii: S0092-8674(21)01496-3. doi: 10.1016/j.cell.2021.12.033."
COMMENT     Sequence Label: pTwist-SARS-CoV-2 Delta-18 B.1.617.2v1
FEATURES             Location/Qualifiers
     source          1..10456
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    49..120
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(125..171)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    189..237
                     /note="synthetic polyadenylation signal"
     enhancer        318..621
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        623..825
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     776..796
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          959..1434
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     misc_feature    5325..5913
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5378..5398)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5796..5807)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    5966..6087
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5977..5996)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6031..6050
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      6202..6248
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      6278..6414
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    6459..6530
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     promoter        6575..6771
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6622..6757
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6785..7579
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6839..6858)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7449..7468
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7590..7814
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(8010..8598)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8090..8109)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(9344..9412,9413..10204)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9562..9581)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      10217..10353
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
ORIGIN
        1 cgtcagctat cgtcagctta cctttttggc agcgatcgcg gctcccgaca tcttggacca
       61 ttagctccac aggtatcttc ttccctctag tggtcataac agcagcttca gctacctctc
      121 aattcaaaaa acccctcaag acccgtttag aggccccaag gggttatgct atcaatcgtt
      181 gcgttacaca cacaaaaaac caacacacat ccatcttcga tggatagcga ttttattatc
      241 taactgctga tcgagtgtag ccagatctag taatcaatta cggggtcatt agttcatagc
      301 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
      361 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
      421 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
      481 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
      541 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
      601 ttagtcatcg ctattaccat gctgatgcgg ttttggcagt acatcaatgg gcgtggatag
      661 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
      721 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
      781 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt
      841 cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca ccgggaccga
      901 tccagcctcc cctcgaagct tacatgtggt accgagctcg gatcctgaga acttcagggt
      961 gagtctatgg gacccttgat gttttctttc cccttctttt ctatggttaa gttcatgtca
     1021 taggaagggg agaagtaaca gggtacacat attgaccaaa tcagggtaat tttgcatttg
     1081 taattttaaa aaatgctttc ttcttttaat atactttttt gtttatctta tttctaatac
     1141 tttccctaat ctctttcttt cagggcaata atgatacaat gtatcatgcc tctttgcacc
     1201 attctaaaga ataacagtga taatttctgg gttaaggcaa tagcaatatt tctgcatata
     1261 aatatttctg catataaatt gtaactgatg taagaggttt catattgcta atagcagcta
     1321 caatccagct accattctgc ttttatttta tggttgggat aaggctggat tattctgagt
     1381 ccaagctagg cccttttgct aatcatgttc atacctctta tcttcctccc acagctcctg
     1441 ggcaacgtgc tggtctgtgt gctggcccat cactttggca aaggcggccg ccatgtttgt
     1501 gttcctcgtt ctcctgcccc tcgtttcttc ccaatgcgtg aacctcagaa ctagaacgca
     1561 gctcccaccc gcttacacga actcttttac tcgtggagta tactatcccg ataaagtctt
     1621 taggtccagt gtccttcatt ctactcaaga ccttttcctg ccattcttct caaatgtcac
     1681 ctggttccat gctatccacg tttctggcac caacggaact aaacgatttg ataatcccgt
     1741 cttgccattt aacgatggcg tatactttgc ctcaacggag aagtccaaca ttatccgggg
     1801 atggatattt ggcaccaccc tggactccaa gacacagtct ctcctcattg tgaacaacgc
     1861 caccaatgtc gtcatcaaag tctgtgaatt tcaattttgt aacgatccct ttctcgatgt
     1921 ttattatcat aagaataaca agtcttggat ggagtcagga gtttacagtt ccgctaacaa
     1981 ctgtactttt gagtacgttt cccaaccatt tctcatggat cttgaaggga agcaaggcaa
     2041 cttcaagaat ctccgcgaat ttgtgtttaa gaatatagac ggctacttca aaatttatag
     2101 caagcatacc ccgataaatc tggttcggga tctccctcaa ggcttctccg cgttggaacc
     2161 cctcgtggac ttgccgattg gcatcaacat cacccggttc caaacccttc ttgcccttca
     2221 tcgaagctac ctcacgcctg gcgacagtag tagtggttgg acagcgggcg cagcagccta
     2281 ctatgtggga tatttgcaac cacgcacctt tcttctcaaa tataacgaaa acggaacaat
     2341 taccgatgcc gtagactgtg ccctcgatcc cctgtccgaa actaaatgca cactcaagtc
     2401 cttcacagtt gagaaaggca tataccaaac cagtaacttc cgcgtccaac ccaccgaatc
     2461 cattgttcga ttccctaaca tcaccaattt gtgtcctttc ggtgaagtat ttaatgccac
     2521 tcgatttgca tcagtttatg cctggaatcg caagcgaata tcaaattgtg ttgcagatta
     2581 tagtgtcctg tataatagcg catcattcag cacttttaag tgttatgggg tcagccctac
     2641 gaaattgaat gacttgtgtt ttaccaacgt ttacgcagat tctttcgtca tacgcggcga
     2701 cgaagtccga cagattgcac cggggcaaac cggaaagata gcagactata attataagtt
     2761 gccggatgac ttcaccggct gcgttatagc atggaactcc aataatctcg attcaaaagt
     2821 cggcggcaac tataattatc gttaccgcct gtttcgcaag tcaaatctga agccctttga
     2881 acgtgacatc tccaccgaaa tttatcaagc tggcagcaaa ccatgtaatg gagtggaagg
     2941 gtttaactgt tattttcccc ttcaatcata cggatttcaa ccaacaaatg gggtgggcta
     3001 tcaaccatat cgggtggtag tcttgagctt tgagctgctc cacgcaccgg ctacagtttg
     3061 cggaccgaag aaaagcacta acctggtgaa gaataaatgt gtgaacttca actttaatgg
     3121 gctcaccgga acaggggtgc tgacagaatc aaacaagaaa tttctccctt ttcaacaatt
     3181 tggtagagac attgccgata ccacagatgc cgtccgcgat ccgcaaacac tggaaatcct
     3241 cgatataaca ccctgtagct tcggcggggt cagcgtgata actcccggaa cgaacacatc
     3301 caaccaagtg gctgtcctgt atcaaggtgt caactgtacc gaagtccctg tggcgataca
     3361 cgccgaccaa ctgacaccca cctggagagt ctactcaaca ggttccaatg tctttcaaac
     3421 tagagcgggt tgtctcattg gcgctgagca tgtcaataat tcatacgaat gtgatattcc
     3481 tattggggca ggaatctgtg cgtcttatca aactcaaacg aactcacgtc ggcgcgctcg
     3541 ctcagtagcc agccagtcca taatcgccta taccatgagt ctcggggctg agaactccgt
     3601 ggcttattca aacaattcca tcgcaatccc aactaacttt accattagcg ttaccacgga
     3661 aatcttgccc gtcagcatga ctaagacgag cgtagactgt acgatgtata tctgtgggga
     3721 ttccaccgag tgtagcaacc tcttgcttca atacgggtct ttctgtactc aactcaaccg
     3781 agcattgacc ggaattgcgg tcgaacaaga caagaatacc caagaggtgt tcgcacaagt
     3841 aaagcaaata tacaaaacgc cgccaatcaa agattttggc gggtttaatt ttagccaaat
     3901 actgccagac ccatctaagc cttcaaaacg cagctttatc gaggatctgc tgttcaacaa
     3961 agttacgttg gctgacgcgg gtttcattaa gcagtatggg gattgtttgg gagacattgc
     4021 ggcccgcgac cttatctgtg ctcaaaaatt caatgggttg acagttctgc cccctctgct
     4081 cactgatgag atgattgcac agtatacatc agcattgttg gcaggaacca ttacatccgg
     4141 ctggactttt ggcgcgggtg cggcccttca aatacctttc gctatgcaaa tggcttacag
     4201 attcaatggg attggtgtca cgcaaaatgt attgtatgaa aatcagaagc tgatcgctaa
     4261 tcaatttaac agcgcgattg gcaagatcca agattcactg tcaagcacag cgtccgccct
     4321 cggcaaactc caaaatgttg tgaatcaaaa cgcccaggcc ctgaataccc tcgtgaagca
     4381 actgagttct aacttcggcg caatctccag cgtcctcaac gacatattgt ccagacttga
     4441 taaggtggaa gccgaggtgc aaattgatcg gctcatcaca ggcaggctcc agtcactcca
     4501 aacttatgtg acacaacagt tgatccgggc tgctgagata cgagcaagcg ccaacctggc
     4561 cgccactaaa atgtcagagt gcgtgctcgg gcagtccaaa cgggttgact tttgtggcaa
     4621 gggctatcac ttgatgtcct tcccccaatc cgccccacat ggagtggtct ttctccacgt
     4681 cacttatgtc cctgctcaag agaagaactt tacgacagct ccagcaatct gtcacgatgg
     4741 caaagcgcac tttcccaggg aaggcgtttt cgtcagtaat ggcacgcatt ggtttgtgac
     4801 ccaaaggaat ttctatgaac cccaaatcat aaccactgac aatacatttg tctcaggcaa
     4861 ctgtgatgtg gtcattggga tcgtgaacaa cacggtatat gatcccctgc aacccgagtt
     4921 ggatagcttc aaagaggaat tggataaata ctttaagaat catacttcac ccgatgttga
     4981 tctcggggac atatcaggaa taaatgcaag tgtcgtcaac attcagaaag agattgatcg
     5041 ccttaatgag gtcgccaaga acttgaatga atctttgatc gacttgcaag aactgggcaa
     5101 atacgaacaa tatattaaat ggccctggta catttggttg ggctttatcg ccggacttat
     5161 agctatcgtt atggtcacaa ttatgttgtg ctgtatgact agctgttgtt cctgcttgaa
     5221 aggatgttgc agttgcggat cttgttgtaa ataaaggatc ctctagaagt tgtctcctcc
     5281 tgcactgact gactgataca atcgatttct ggatccgcag gcctaatcaa cctctggatt
     5341 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     5401 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     5461 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     5521 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     5581 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     5641 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     5701 ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct
     5761 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     5821 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     5881 cgagtcggat ctccctttgg gccgcctccc cgcctgtcta gcttgactga ctgagataca
     5941 gcgtaccttc agctcacaga catgataaga tacattgatg agtttggaca aaccacaact
     6001 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta
     6061 accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag
     6121 gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtatt
     6181 ggcccatctc tatcggtatc gtagcataac cccttggggc ctctaaacgg gtcttgaggg
     6241 gttttttgtg cccctcgggc cggattgcta tctaccggca ttggcgcaga aaaaaatgcc
     6301 tgatgcgacg ctgcgcgtct tatactccca catatgccag attcagcaac ggatacggct
     6361 tccccaactt gcccacttcc atacgtgtcc tccttaccag aaatttatcc ttaaggtcgt
     6421 cagctatcct gcaggcgatc tctcgatttc gatcaagaca ttcctttaat ggtcttttct
     6481 ggacaccact aggggtcaga agtagttcat caaactttct tccctcccta atctcattgg
     6541 ttaccttggg ctatcgaaac ttaattaagc gatctgcatc tcaattagtc agcaaccata
     6601 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     6661 ccccatcgct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     6721 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aggaggtagc
     6781 caacatgatt gaacaagatg gattgcacgc aggttctccc gccgcttggg tggagaggct
     6841 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct
     6901 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga
     6961 actccaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc
     7021 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg
     7081 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc
     7141 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca
     7201 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga
     7261 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgcggatgcc
     7321 cgacggcgag gatctcgtcg tgacccacgg cgatgcctgc ttgccgaata tcatggtgga
     7381 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca
     7441 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg
     7501 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct
     7561 tcttgacgag ttcttctagt atgtaagccc tgtgccttct agttgccagc catctgttgt
     7621 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     7681 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     7741 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
     7801 ggtgggctct atggttaatt aaccagtcaa gtcagctact tggcgagatc gacttgtctg
     7861 ggtttcgact acgctcagaa ttgcgtcagt caagttcgat ctggtccttg ctattgcacc
     7921 cgttctccga ttacgagttt catttaaatc atgtgagcaa aaggccagca aaaggccagg
     7981 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     8041 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     8101 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     8161 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     8221 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     8281 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     8341 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     8401 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     8461 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     8521 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     8581 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     8641 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     8701 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     8761 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     8821 tcatccatag ttgcatttaa atttccgaac tctccaaggc cctcgtcgga aaatcttcaa
     8881 acctttcgtc cgatccatct tgcaggctac ctctcgaacg aactatcgca agtctcttgg
     8941 ccggccttgc gccttggcta ttgcttggca gcgcctatcg ccaggtatta ctccaatccc
     9001 gaatatccga gatcgggatc acccgagaga agttcaacct acatcctcaa tcccgatcta
     9061 tccgagatcc gaggaatatc gaaatcgggg cgcgcctggt gtaccgagaa cgatcctctc
     9121 agtgcgagtc tcgacgatcc atatcgttgc ttggcagtca gccagtcgga atccagcttg
     9181 ggacccagga agtccaatcg tcagatattg tactcaagcc tggtcacggc agcgtaccga
     9241 tctgtttaaa cctagatatt gatagtctga tcggtcaacg tataatcgag tcctagcttt
     9301 tgcaaacatc tatcaagaga caggatcagc aggaggcttt cgcatgagta ttcaacattt
     9361 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     9421 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcgcgagtgg gttacatcga
     9481 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gctttccaat
     9541 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     9601 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt attcaccagt
     9661 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     9721 catgagtgat aacactgcgg ccaacttact tctgacaacg attggaggac cgaaggagct
     9781 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     9841 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     9901 aaccttgcgt aaactattaa ctggcgaact acttactcta gcttcccggc aacagttgat
     9961 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
    10021 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
    10081 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
    10141 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
    10201 gtaaccgatt ctaggtgcat tggcgcagaa aaaaatgcct gatgcgacgc tgcgcgtctt
    10261 atactcccac atatgccaga ttcagcaacg gatacggctt ccccaacttg cccacttcca
    10321 tacgtgtcct ccttaccaga aatttatcct taagatcccg aatcgtttaa actcgactct
    10381 ggctctatcg aatctccgtc gtttcgagct tacgcgaaca gccgtggcgc tcatttgctc
    10441 gtcgggcatc gaatct
//
