LOCUS       sequence-351286-       10552 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian lentiviral expression vector encoding GNAS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10552)
  AUTHORS   Moyer AJ, Fernandez FX, Li Y, Klinedinst DK, Florea LD, Kazuki Y, 
            Oshimura M, Reeves RH
  TITLE     Overexpression screen of chromosome 21 genes reveals modulators of 
            Sonic hedgehog signaling relevant to Down syndrome.
  JOURNAL   Dis Model Mech. 2023 Apr 1;16(4):dmm049712. doi: 10.1242/dmm.049712.
            Epub 2023 Apr 13.
  PUBMED    36995257
REFERENCE   2  (bases 1 to 10552)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dis Model 
            Mech."; date: "2023-04-1"; volume: "16(4):dmm049712. doi"; pages: " 
            10.1242/dmm.049712. Epub 2023 Apr 13"
COMMENT     Sequence Label: GNAS_pLENTI-CAG-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..10552
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          122..1138
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1062..1084
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1146..1165
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    1254..1278
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2521..2545)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2565..2588
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    2623..3172
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2767..2784)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2994..3013
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(3175..3177,3178..3180,3181..3894)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3175..3177,3178..3180,3181..3891)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3220..3241)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3481..3500)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3828..3849
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    3971..4559
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4024..4044)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4442..4453)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4562..4578)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4563..4579)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4768..5001
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5073..5194
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5110..5129)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5164..5183
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5234..5369
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5389..5408)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5390..5408)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(5431..5789)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5471..5492)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5683..5702
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5816..5920
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5921..5989,5990..6781)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPKLNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6139..6158)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6952..7540
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7441..7460
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        7715..8018
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8020..8218
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8173..8193
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             8236..8416
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8463..8588
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9085..9318
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9503..9547
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9845..9962
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        10060..10439
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
       61 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      121 gggagtcgct gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc
      181 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
      241 tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga
      301 aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg
      361 cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg
      421 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg
      481 gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt
      541 gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca
      601 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg
      661 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc
      721 ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccc ggagcgccgg
      781 cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc
      841 gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac
      901 cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga
      961 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc
     1021 gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt
     1081 gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
     1141 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tcccctagcc
     1201 gcggaatcta gactgccgga tcgaattgtc gacgaattcg gatctggcca atcacaagtt
     1261 tgtacaaaaa agcaggctcc gcggccgccc ccttcaccat gggctgcctc gggaacagta
     1321 agaccgagga ccagcgcaac gaggagaagg cgcagcgtga ggccaacaaa aagatcgaga
     1381 agcagctgca gaaggacaag caggtctacc gggccacgca ccgcctgctg ctgctgggtg
     1441 ctggagaatc tggtaaaagc accattgtga agcagatgag gatcctgcat gttaatgggt
     1501 ttaatggaga gggcggcgaa gaggacccgc aggctgcaag gagcaacagc gatggcagtg
     1561 agaaggcaac caaagtgcag gacatcaaaa acaacctgaa agaggcgatt gaaaccattg
     1621 tggccgccat gagcaacctg gtgccccccg tggagctggc caaccccgag aaccagttca
     1681 gagtggacta catcctgagt gtgatgaacg tgcctgactt tgacttccct cccgaattct
     1741 atgagcatgc caaggctctg tgggaggatg aaggagtgcg tgcctgctac gaacgctcca
     1801 acgagtacca gctgattgac tgtgcccagt acttcctgga caagatcgac gtgatcaagc
     1861 aggctgacta tgtgccgagc gatcaggacc tgcttcgctg ccgtgtcctg acttctggaa
     1921 tctttgagac caagttccag gtggacaaag tcaacttcca catgtttgac gtgggtggcc
     1981 agcgcgatga acgccgcaag tggatccagt gcttcaacga tgtgactgcc atcatcttcg
     2041 tggtggccag cagcagctac aacatggtca tccgggagga caaccagacc aaccgcctgc
     2101 aggaggctct gaacctcttc aagagcatct ggaacaacag atggctgcgc accatctctg
     2161 tgatcctgtt cctcaacaag caagatctgc tcgctgagaa agtccttgct gggaaatcga
     2221 agattgagga ctactttcca gaatttgctc gctacactac tcctgaggat gctactcccg
     2281 agcccggaga ggacccacgc gtgacccggg ccaagtactt cattcgagat gagtttctga
     2341 ggatcagcac tgccagtgga gatgggcgtc actactgcta ccctcatttc acctgcgctg
     2401 tggacactga gaacatccgc cgtgtgttca acgactgccg tgacatcatt cagcgcatgc
     2461 accttcgtca gtacgagctg ctctactagc caactttctt gtacaagggt gggcgcgccg
     2521 acccagcttt cttgtacaaa gtggtgggag gaggtggatc tggcgactac aaggacgacg
     2581 atgacaagtg actcgagcgg gatcaattcc gccccccccc taacgttact ggccgaagcc
     2641 gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt
     2701 ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc
     2761 tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc
     2821 tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc
     2881 cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg
     2941 cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct
     3001 cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat
     3061 ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaaacgtc
     3121 taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgataa taccatggtg
     3181 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     3241 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
     3301 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     3361 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
     3421 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
     3481 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     3541 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     3601 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc
     3661 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
     3721 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     3781 agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     3841 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaaagcggc
     3901 cgcgactcta gagtcgacct gcaggcatgc aagcttcagg tagccggcta acgttatcaa
     3961 gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     4021 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     4081 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     4141 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     4201 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     4261 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     4321 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
     4381 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     4441 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     4501 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca
     4561 tcgataccgt cgacctcgat cgagacctag aaaaacatgg agcaatcaca agtagcaata
     4621 cagcagctac caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt
     4681 ttccagtcac acctcaggta cctttaagac caatgactta caaggcagct gtagatctta
     4741 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
     4801 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     4861 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     4921 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     4981 cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag tatttataac
     5041 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt
     5101 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     5161 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc
     5221 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     5281 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     5341 gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag
     5401 tcgtattagc atgtgagcaa aaggccagca taaatcaaaa gaatagaccg agatagggtt
     5461 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa
     5521 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag
     5581 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt
     5641 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg
     5701 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc
     5761 cgcgcttaat gcgccgctac agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg
     5821 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     5881 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     5941 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     6001 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     6061 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     6121 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     6181 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     6241 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     6301 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     6361 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccgaagct
     6421 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     6481 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     6541 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     6601 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     6661 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     6721 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     6781 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     6841 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     6901 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     6961 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     7021 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     7081 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     7141 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     7201 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     7261 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     7321 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     7381 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     7441 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     7501 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     7561 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     7621 tgattctgtg gataaccgta ttaccgccat gcattagtta ttaatagtaa tcaattacgg
     7681 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
     7741 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
     7801 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
     7861 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
     7921 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
     7981 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
     8041 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
     8101 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
     8161 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagcg
     8221 cgttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     8281 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     8341 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     8401 tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg
     8461 agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc
     8521 gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg
     8581 agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc
     8641 cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac
     8701 gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac
     8761 agctacaacc atcccttcag acaggatcag aagaacttag atcattatat aatacagtag
     8821 caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca
     8881 agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc ggccgctgat
     8941 cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa
     9001 agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca
     9061 gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg
     9121 aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg
     9181 tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca
     9241 actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct
     9301 aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc
     9361 tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac
     9421 ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga
     9481 agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc
     9541 aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat
     9601 gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag
     9661 agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc
     9721 cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg
     9781 attagtgaac ggatcggcac tgcgtgcgcc aattctgcag acaaatggca gtattcatcc
     9841 acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
     9901 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt
     9961 ttcgggttta ttacagggac agcagagatc cagtttggtt agtaccgggc ccgctctagc
    10021 cctagattta aatcgatgta cgggccagat atacgcgttg acattgatta ttgactagtt
    10081 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
    10141 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
    10201 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
    10261 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
    10321 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
    10381 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
    10441 tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc ccacccccaa
    10501 ttttgtattt atttattttt taattatttt gtgcagcgat gggggcgggg gg
//
