LOCUS       sequence-351299-        8881 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plant Gene Expression Vector pSIM24 containing the GUS 
            (Beta-glucuronidase) reporter gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8881)
  AUTHORS   Sahoo DK, Dey N, Maiti IB
  TITLE     pSiM24 is a novel versatile gene expression vector for transient 
            assays as well as stable expression of foreign genes in plants.
  JOURNAL   PLoS One. 2014 Jun 4;9(6):e98988. doi: 10.1371/journal.pone.0098988.
            eCollection 2014.
  PUBMED    24897541
REFERENCE   2  (bases 1 to 8881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-06-4"; volume: "9(6):e98988. doi"; pages: " 
            10.1371/journal.pone.0098988. eCollection 2014"
COMMENT     Sequence Label: pSIM24-GUS
FEATURES             Location/Qualifiers
     source          1..8881
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      18..659
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     promoter        complement(1739..1918)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    2153..2177
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(2292..2880)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2372..2391)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3051..3842,3843..3911))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFCVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3674..3693
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3912..4016)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4057..4667
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
     rep_origin      4144..4675
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    6247..6271
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7061..8875
                     /codon_start=1
                     /gene="uidA (or gusA) from E. coli"
                     /product="beta-glucuronidase"
                     /label=GUS
                     /translation="MGLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQE
                     VMEHQGGYTPFEADVAPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFH
                     DFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQ
                     QVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQ
                     FLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLD
                     WADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIAR
                     DKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTIS
                     DLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSM
                     YTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDR
                     KPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
ORIGIN
        1 gagctcctgc agtctagagc tttcgttcgt atcatcggtt tcgacaacgt tcgtcaagtt
       61 caatgcatca gtttcattgc gcacacacca gaatcctact gagtttgagt attatggcat
      121 tgggaaaact gtttttcttg taccatttgt tgtgcttgta atttactgtg ttttttattc
      181 ggttttcgct atcgaactgt gaaatggaaa tggatggaga agagttaatg aatgatatgg
      241 tccttttgtt cattctcaaa ttaatattat ttgttttttc tcttatttgt tgtgtgttga
      301 atttgaaatt ataagagata tgcaaacatt ttgttttgag taaaaatgtg tcaaatcgtg
      361 gcctctaatg accgaagtta atatgaggag taaaacactt gtagttgtac cattatgctt
      421 attcactagg caacaaatat attttcagac ctagaaaagc tgcaaatgtt actgaataca
      481 agtatgtcct cttgtgtttt agacatttat gaactttcct ttatgtaatt ttccagaatc
      541 cttgtcagat tctaatcatt gctttataat tatagttata ctcatggatt tgtagttgag
      601 tatgaaaata ttttttaatg cattttatga cttgccaatt gattgacaac atgcatcaat
      661 cgatactagt ctagtaacat agatgacacc gcgagcgata atttatccta gtttgcgagc
      721 tatattttgt tttctatcgc gtattaaatg tataattgcg ggactctaat cataaaaacc
      781 catctcataa ataacgtcat gcattacatg ttaattatta catgcttaac gtaattcaac
      841 agaaattata tgataatcat cgcaagaccg gcaacaggat tcaatcttaa gaaactttat
      901 tgccaaatgt ttgaacgatt cagaagaact catcgaggag cctgtagaaa gcaatccttt
      961 gggaatctgg agcagcaatt ccgtaaagca caaggaatct atcagcccac tctccaccaa
     1021 gttcttcagc aatatcccta gtagcgagag caatatcctg atacctatca gcaactccaa
     1081 gccttccgca atcaatgaat ccagaaaacc ttccgttctc caccataatg tttggaaggc
     1141 aagcatcacc atgagtaaca acaagatcct caccatctgg cattctagcc ttgagcctag
     1201 caaaaagttc agctggagcc aatccttgat gttcctcatc aagatcatcc tgatccacaa
     1261 gtccagcttc catcctagtt ctagcccttt caattctgtg cttagcttgg tgatcaaatg
     1321 ggcaagtagc aggatcaaga gtgtgaagcc ttctcatagc atcagccata atggaaacct
     1381 tctcagctgg agcaaggtgt gaagaaagca aatcttgtcc tggaacctct cccaaaagaa
     1441 gccaatctct tccagcctca gtaacaacat caagaacagc agcgcatgga acaccagtag
     1501 tagcaagcca agaaagtcta gcagcctcat cttgaagctc attaagagca ccagaaagat
     1561 cagtcttaac gaaaagaact ggccttccct gagcagaaag cctaaaaaca gcagcatcag
     1621 agcatccaat agtctgttga gcccaatcgt atccaaaaag cctctcaacc caagcagcag
     1681 gacttccagc atgaagtcca tcttgctcaa ttcccatttg agagtgaata tgagactcta
     1741 attggatacc gaggggaatt tatggaacgt cagtggagca tttttgacaa gaaatatttg
     1801 ctagctgata gtgaccttag gcgacttttg aacgcgcaat aatggtttct gacgtatgtg
     1861 cttagctcat taaactccag aaacccgcgg ctcagtggct ccttcaacgt tgcggttctg
     1921 tcagttccaa acgtaaaacg gcttgtcccg cgtcatcggc gggggtcata acgtgactcc
     1981 cttaattctc cgctcatgat cagatctggt gacccctatg taaggggccg gccatttaaa
     2041 ttcgcgatta attaacctgt ggttggcatg cacatacaaa tggacgaacg gataaacctt
     2101 ttcacgccct tttaaatatc cgattattct aataaacgct cttttctctt aggtttaccc
     2161 gccaatatat cctgtcaaac actgatagtt taaactgaag gcgggaaacg acaatctgat
     2221 atcgcgcgcg gtacctgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     2281 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     2341 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     2401 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     2461 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     2521 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     2581 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     2641 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     2701 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     2761 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     2821 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     2881 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     2941 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     3001 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     3061 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     3121 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     3181 atgataccgc gagaaccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     3241 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     3301 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     3361 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     3421 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     3481 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     3541 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     3601 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     3661 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     3721 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     3781 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     3841 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acagaaatgt
     3901 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     3961 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     4021 tttccccgaa aagtgccacg ggccccgctc accgggctgg ttgccctcgc cgctgggctg
     4081 gcggccgtct atggccctgc aaacgcgcca gaaacgccgt cgaagccgtg tgcgagacac
     4141 cgcggccgcc ggcgttgtgg atacctcgcg gaaaacttgg ccctcactga cagatgaggg
     4201 gcggacgttg acacttgagg ggccgactca cccggcgcgg cgttgacaga tgaggggcag
     4261 gctcgatttc ggccggcgac gtggagctgg ccagcctcgc aaatcggcga aaacgcctga
     4321 ttttacgcga gtttcccaca gatgatgtgg acaagcctgg ggataagtgc cctgcggtat
     4381 tgacacttga ggggcgcgac tactgacaga tgaggggcgc gatccttgac acttgagggg
     4441 cagagtgctg acagatgagg ggcgcaccta ttgacatttg aggggctgtc cacaggcaga
     4501 aaatccagca tttgcaaggg tttccgcccg tttttcggcc accgctaacc tgtcttttaa
     4561 cctgctttta aaccaatatt tataaacctt gtttttaacc agggctgcgc cctgtgcgcg
     4621 tgaccgcgca cgccgaaggg gggtgccccc ccttctcgaa ccctcccggc ccgctgtcga
     4681 ctacctagat gtggcgcaac gatgccggcg acaagcagga gcgcaccgac ttcttccgca
     4741 tcaagtgttt tggctctcag gccgaggccc acggcaagta tttgggcaag gggtcgctgg
     4801 tattcgtgca gggcaagatt cggaatacca agtacgagaa ggacggccag acggtctacg
     4861 ggaccgactt cattgccgat aaggtggatt atctggacac caaggcacca ggcgggtcaa
     4921 atcaggaata agggcacatt gccccggcgt gagtcggggc aatcccgcaa ggagggtgaa
     4981 tgaatcgcac cttcgatcgc aaggcgtatc gccaggaact gatcgacgcc ggcttctcgg
     5041 ccgaagatgc cgaaacgatc gcctcgcgca ccgtcatgcg tgccccgcgc gaaaccttcc
     5101 agtcggtcgg ctcgatggtc cagcaggcca ccgccaagat cgaacgcgat tcggtccagc
     5161 tggccccgcc ggcccttccg gccccgtcgg ccgccgtcga acgctcgcgc cggctggaac
     5221 aggaagccgc cggcctggcc aagtcgatga ccatcgacac ccgcggcacc atgaccacca
     5281 agaagcgcaa gaccgccggc gaggatctgg ccaagcaggt cagcgaagcc aagcaggccg
     5341 ccctgctgaa gcataccaag cagcagatca aggaaatgca gctgtcgctg ttcgacatcg
     5401 ccccgtggcc ggataccatg cgggccatgc cgaatgatac cgcccgctcg gccctgttca
     5461 ccacccgcaa taagaagatc ccgcgcgaag ccctccagaa taaggtcatc ttccacgtca
     5521 acaaggatgt caagatcacc tataccggcg tcgaactgcg ggccgatgat gatgaactgg
     5581 tctggcagca ggtcctggaa tatgccaagc gcaccccgat cggcgaaccg atcaccttca
     5641 ccttctatga actgtgccag gatctgggct ggtcgatcaa tggccgctat tataccaagg
     5701 ccgaagaatg cctgtcgcgc ctccaggcga ccgcgatggg cttcacctcg gatcgcgtcg
     5761 gccatctgga atcggtcagc ctgctgcacc gcttccgcgt cctggatcgc ggcaagaaga
     5821 cctcgcgctg ccaggtcctg atcgacgaag aaatcgtcgt cctgttcgcc ggcgatcatt
     5881 ataccaagtt catctgggag aagtatcgca agctgtcgcc gaccgcccgc cgcatgttcg
     5941 attatttctc gtcgcaccgc gaaccgtatc cgctgaagct ggaaaccttc cgcctgatgt
     6001 gcggctcgga ttcgacccgc gtcaagaagt ggcgcgaaca ggtcggcgaa gcctgcgaag
     6061 aactgcgcgg ctcgggcctg gtcgaacatg cctgggtcaa tgatgatctg gtccattgca
     6121 agcgctaggg ccttgtgggg tcagttccgg ctgggggttc agccagcgct ttacgatatc
     6181 ggcgcgcggt acccagtaca ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt
     6241 caatttgttt acaccacaat atatcctgcc accagccagc caacagctcc ccgaccggca
     6301 gctcggcaca aaatcaccac tcgatacagg cagcccatca gtccactaga ccagtacctt
     6361 ggaggcctgt gcgcatccca gggtgggtcc cgtatacccc ggggaattcg tcaacttcgt
     6421 ccacagacat caacatctta tcgtcctttg aagataagat aataatgttg aagataagag
     6481 tgggagcccc cactaaaaca ttgctttgtc aaaagctaaa aaagatgatg cccgacagcc
     6541 acttgtgtga agcatgagaa gccggtccct ccactaagaa aattagtgaa gcatcttcca
     6601 gtggtccctc cactcacagc tcaatcagtg agcaacagga cgaaggaaat gacgtaagcc
     6661 atgacgtcta atcccaactt cgtccacaga catcaacatc ttatcgtcct ttgaagataa
     6721 gataataatg ttgaagataa gagtgggagc caccactaaa acattgcttt gtcaaaagct
     6781 aaaaaagatg atgcccgaca gccacttgtg tgaagcatga gaagccggtc cctccactaa
     6841 gaaaattagt gaagcatctt ccagtggtcc ctccactcac agctcaatca gtgagcaaca
     6901 ggacgaagga aatgacgtaa gccatgacgt ctaatcccac aagaatttcc ttatataagg
     6961 aacacaaatc agaaggaaga gatcaatcga aatcaaaatc ggaatcgaaa tcaaaatcgg
     7021 aatcgaaatc tctcatctaa gcttggatcc ctcgagaacc atgggtttac gtcctgtaga
     7081 aaccccaacc cgtgaaatca aaaaactcga cggcctgtgg gcattcagtc tggatcgcga
     7141 aaactgtgga attgatcagc gttggtggga aagcgcgtta caagaaagcc gggcaattgc
     7201 tgtgccaggc agttttaacg atcagttcgc cgatgcagat attcgtaatt atgcgggcaa
     7261 cgtctggtat cagcgcgaag tctttatacc gaaaggttgg gcaggccagc gtatcgtgct
     7321 gcgtttcgat gcggtcactc attacggcaa agtgtgggtc aataatcagg aagtgatgga
     7381 gcatcagggc ggctatacgc catttgaagc cgatgtcgcg ccgtatgtta ttgccgggaa
     7441 aagtgtacgt atcaccgttt gtgtgaacaa cgaactgaac tggcagacta tcccgccggg
     7501 aatggtgatt accgacgaaa acggcaagaa aaagcagtct tacttccatg atttctttaa
     7561 ctatgccgga atccatcgca gcgtaatgct ctacaccacg ccgaacacct gggtggacga
     7621 tatcaccgtg gtgacgcatg tcgcgcaaga ctgtaaccac gcgtctgttg actggcaggt
     7681 ggtggccaat ggtgatgtca gcgttgaact gcgtgatgcg gatcaacagg tggttgcaac
     7741 tggacaaggc actagcggga ctttgcaagt ggtgaatccg cacctctggc aaccgggtga
     7801 aggttatctc tatgaactgt gcgtcacagc caaaagccag acagagtgtg atatctaccc
     7861 gcttcgcgtc ggcatccggt cagtggcagt gaagggcgaa cagttcctga ttaaccacaa
     7921 accgttctac tttactggct ttggtcgtca tgaagatgcg gacttgcgtg gcaaaggatt
     7981 cgataacgtg ctgatggtgc acgaccacgc attaatggac tggattgggg ccaactccta
     8041 ccgtacctcg cattaccctt acgctgaaga gatgctcgac tgggcagatg aacatggcat
     8101 cgtggtgatt gatgaaactg ctgctgtcgg ctttaacctc tctttaggca ttggtttcga
     8161 agcgggcaac aagccgaaag aactgtacag cgaagaggca gtcaacgggg aaactcagca
     8221 agcgcactta caggcgatta aagagctgat agcgcgtgac aaaaaccacc caagcgtggt
     8281 gatgtggagt attgccaacg aaccggatac ccgtccgcaa ggtgcacggg aatatttcgc
     8341 gccactggcg gaagcaacgc gtaaactcga cccgacgcgt ccgatcacct gcgtcaatgt
     8401 aatgttctgc gacgctcaca ccgataccat cagcgatctc tttgatgtgc tgtgcctgaa
     8461 ccgttattac ggatggtatg tccaaagcgg cgatttggaa acggcagaga aggtactgga
     8521 aaaagaactt ctggcctggc aggagaaact gcatcagccg attatcatca ccgaatacgg
     8581 cgtggatacg ttagccgggc tgcactcaat gtacaccgac atgtggagtg aagagtatca
     8641 gtgtgcatgg ctggatatgt atcaccgcgt ctttgatcgc gtcagcgccg tcgtcggtga
     8701 acaggtatgg aatttcgccg attttgcgac ctcgcaaggc atattgcgcg ttggcggtaa
     8761 caagaaaggg atcttcactc gcgaccgcaa accgaagtcg gcggcttttc tgctgcaaaa
     8821 acgctggact ggcatgaact tcggtgaaaa accgcagcag ggaggcaaac aatgagtcga
     8881 c
//
