LOCUS       sequence-351317-        9367 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses KSHV ORF 10 tagged.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9367)
  AUTHORS   Alzhanova D, Corcoran K, Bailey AG, Long K, Taft-Benz S, Graham RL, 
            Broussard GS, Heise M, Neumann G, Halfmann P, Kawaoka Y, Baric RS, 
            Damania B, Dittmer DP
  TITLE     Novel modulators of p53-signaling encoded by unknown genes of 
            emerging viruses.
  JOURNAL   PLoS Pathog. 2021 Jan 7;17(1):e1009033. doi: 
            10.1371/journal.ppat.1009033. eCollection 2021 Jan.
  PUBMED    33411764
REFERENCE   2  (bases 1 to 9367)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Pathog."; date: "2021-01-7"; volume: "17(1):e1009033. doi"; pages: "
            10.1371/journal.ppat.1009033. eCollection 2021 Jan"
COMMENT     Sequence Label: pOME0004L
FEATURES             Location/Qualifiers
     source          1..9367
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1804..1921
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        1944..2247
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2248..2451
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2402..2422
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2448..2472
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2568..2592
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3884..3949
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(3985..4009)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4062..4103
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    4127..4715
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4180..4200)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4598..4609)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4730..5046
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4897..5032
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             join(5097..5099,5100..5102,5103..5816)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5142..5163)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5403..5422)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5750..5771
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             5888..6121
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6193..6314
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6230..6249)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6284..6303
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6354..6489
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6509..6528)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6510..6528)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6538..6555)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6538..6554)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6547..6569)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6696..7151
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6783..6802)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6993..7014
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7177..7281
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7282..7350,7351..8142)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7500..7519)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8313..8901
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8802..8821
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9055..9072
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9189..9210
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9225..9230,9231..9248,9249..9255)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9263..9279
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9268..9290
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9287..9303
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9287..9303
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9322..9342
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9324..9342
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgataag cttgggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
     1981 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
     2041 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
     2101 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
     2161 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
     2221 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
     2281 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
     2341 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
     2401 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg
     2461 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccga
     2521 ctctagagga tccactagtc cagtgtggtg gaattctgca gatatcaaca agtttgtaca
     2581 aaaaagcagg ctctttaaag gaaccaattc agtcgacggt accgccacca tgcagacaga
     2641 ggcaacgttc atcctaggtg actgggagat aacggtgtct aactgccggt ttacttgcag
     2701 cagcctaaca tgtggccccc tttacagatc tagcggcgac tacacgcggc taagaatccc
     2761 cttctctctg gatcgactaa tacgtgacca tgccatcttt gggctagtgc caaatattga
     2821 ggatctgtta acccatgggt catgcgtcgc cgtagtggcc gacgcaaacg ccacaggcgg
     2881 caacgcgcga cgcatcgtcg cgcctggcgt gataaacaat ttttcagaac ccatcggcat
     2941 ttgggtacgc ggccctccgc cgcaaacgcg caaggaagct attaagttct gcatattttt
     3001 tgtcagtccc atgcccccgc gggagatgac cacatatgtg ttcaagggcg gcgatttgcc
     3061 tcccggagca gaggaacccg aaacactaca ctccgccgag gcacccctac cgtcgcgcga
     3121 gacgctggta actggacagc tgcgatccac ctcgccgcga acgtatacgg gatactttca
     3181 cagtcctgtc ccgctctctt ttttggacct cctgacattc gagtccattg ggtgtgacaa
     3241 cgtggaaggt gaccccgagc aattgacacc caagtacttg acgttcacgc agacgggaga
     3301 aagactttgc aaagtaaccg tttacaacac ccattcgaca gcatgcaaga aggcccgtgt
     3361 tcgtttcgtc tacagaccga cgccgtccgc ccgtcagctt gtcatgggtc aggcttcacc
     3421 cctcataaca acccctctgg gagccagggt attcgcagtc tatccagact gtgagaaaac
     3481 tatcccacct caggaaacca ccaccctgag gattcaattg ctgttcgagc agcatggtgc
     3541 caacgccgga gactgcgcct ttgtcatcat ggggctcgcc cgtgaaacaa agtttgtctc
     3601 atttcccgca gtactccttc cgggcaagca cgaacacctt attgtattca acccacagac
     3661 acatcctctg accattcaac gggacacaat agtgggcgtg gcaatggctt gctatatcca
     3721 ccccggtaag gcagccagcc aggcaccata cagcttctac gactgcaagg aagagagctg
     3781 gcacgtgggg ctcttccaga tcaaacgcgg accgggaggg gtctgtacac caccttgcca
     3841 cgtagcgatt agggccgacc gccacgagga acccatgcaa tcggactaca aagaccatga
     3901 cggtgattat aaagatcatg atatcgatta caaggatgac gatgacaagt agtaactcga
     3961 ggcggccgca ctcgagatat ctagacccag ctttcttgta caaagtggtt gatatccagc
     4021 acagtggcgg ccgctcgagt ctagagggcc cgcggttcga aggtaagcct atccctaacc
     4081 ctctcctcgg tctcgattct acgcgtaccg gttagtaatg atcgacaatc aacctctgga
     4141 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     4201 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     4261 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     4321 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     4381 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     4441 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     4501 ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac
     4561 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     4621 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     4681 gacgagtcgg atctcccttt gggccgcctc cccgcctggc gatggtaccg gtgtggaaag
     4741 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     4801 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     4861 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     4921 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     4981 gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     5041 tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcagca cctagcatgg
     5101 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
     5161 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
     5221 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
     5281 tgaccacctt cacctacggc gtgcagtgct tcgcccgcta ccccgaccac atgaagcagc
     5341 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
     5401 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
     5461 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
     5521 tggagtacaa ctacaacagc cacaaggtct atatcaccgc cgacaagcag aagaacggca
     5581 tcaaggtgaa cttcaagacc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
     5641 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     5701 tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     5761 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaaggta
     5821 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     5881 gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact
     5941 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     6001 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     6061 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     6121 agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca
     6181 gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     6241 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     6301 atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc
     6361 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     6421 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     6481 ttggaggcct agggacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact
     6541 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
     6601 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
     6661 ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag
     6721 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     6781 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     6841 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     6901 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     6961 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     7021 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     7081 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     7141 gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     7201 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     7261 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     7321 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     7381 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     7441 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     7501 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     7561 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     7621 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     7681 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     7741 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     7801 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     7861 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     7921 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     7981 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     8041 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     8101 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     8161 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     8221 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     8281 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     8341 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     8401 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     8461 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     8521 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     8581 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     8641 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     8701 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     8761 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     8821 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     8881 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     8941 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     9001 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     9061 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     9121 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     9181 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
     9241 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
     9301 gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg
     9361 caagctt
//
