LOCUS       sequence-351323-        9073 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses KSHV K3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9073)
  AUTHORS   Alzhanova D, Corcoran K, Bailey AG, Long K, Taft-Benz S, Graham RL, 
            Broussard GS, Heise M, Neumann G, Halfmann P, Kawaoka Y, Baric RS, 
            Damania B, Dittmer DP
  TITLE     Novel modulators of p53-signaling encoded by unknown genes of 
            emerging viruses.
  JOURNAL   PLoS Pathog. 2021 Jan 7;17(1):e1009033. doi: 
            10.1371/journal.ppat.1009033. eCollection 2021 Jan.
  PUBMED    33411764
REFERENCE   2  (bases 1 to 9073)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Pathog."; date: "2021-01-7"; volume: "17(1):e1009033. doi"; pages: "
            10.1371/journal.ppat.1009033. eCollection 2021 Jan"
COMMENT     Sequence Label: pOME0008L
FEATURES             Location/Qualifiers
     source          1..9073
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1804..1921
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        1944..2247
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2248..2451
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2402..2422
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2448..2472
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2568..2592
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      2624..2633
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3596..3661
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(3691..3715)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3768..3809
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3833..4421
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3886..3906)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4304..4315)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4436..4752
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4603..4738
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             join(4803..4805,4806..4808,4809..5522)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4848..4869)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5109..5128)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5456..5477
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             5594..5827
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5899..6020
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5936..5955)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5990..6009
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6060..6195
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6215..6234)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6216..6234)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6244..6261)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6244..6260)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6253..6275)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6402..6857
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6489..6508)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6699..6720
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6883..6987
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6988..7056,7057..7848)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7206..7225)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8019..8607
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8508..8527
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8761..8778
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8895..8916
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8931..8936,8937..8954,8955..8961)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8969..8985
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8974..8996
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8993..9009
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8993..9009
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9028..9048
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9030..9048
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgataag cttgggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
     1981 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
     2041 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
     2101 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
     2161 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
     2221 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
     2281 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
     2341 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
     2401 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg
     2461 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccga
     2521 ctctagagga tccactagtc cagtgtggtg gaattctgca gatatcaaca agtttgtaca
     2581 aaaaagcagg ctctttaaag gaaccaattc agtcgacggt accgccacca tggaagatga
     2641 ggatgttcct gtctgctgga tttgcaacga ggagctcgga aatgagagat ttagagcctg
     2701 tggatgcaca ggagagctcg agaacgtcca tagaagttgt ttaagcacct ggctcactat
     2761 ctctagaaac acggcctgtc agatatgtgg cgtcgtatac aacacgcgcg tggtctggcg
     2821 acccttgcgc gaaatgacgc tattgcctcg gctgacttac caggagggtc tggaactgat
     2881 tgtttttatt ttcattatga cattgggggc cgctggcctt gccgctgcga cgtgggtttg
     2941 gctatatata gtgggcggtc atgacccaga gatagatcac gtcgctgcag ccgcgtacta
     3001 cgtttttttc gtgttttacc aattgtttgt cgtttttggg ttgggtgcgt ttttccacat
     3061 gatgcgccac gtggggcggg catacgctgc tgtaaacacg cgggttgaag tgtttccata
     3121 tagacctcgg ccgacatcac cagagtgtgc agtagaggag atcgagcttc aggaaattct
     3181 tccccgtggg gataaccagg acgaggaggg gcccgcgggg gcagctccag gcgaccaaga
     3241 tggccccgcg gatggcgctc ctgtgcatcg cgactcagaa gaatccgtgg atgaagctgc
     3301 agggtacaag gaagcgggag aaccaacaca taatgatgga cgtgatgaca atgtagagcc
     3361 aaccgcggtt gggtgtgact gtaacaactt gggcgctgag cggtataggg ccacttactg
     3421 tggcggttat gttggtgccc agtcgggcga tggggcttat agtgtctcct gccataataa
     3481 ggctggaccc tcctctctag ttgatatcct tccacagggt ttgcctgggg gtggctatgg
     3541 ttccatgggc gtgattagga aacgttcggc tgtttcgtct gcccttatgt ttcatgacta
     3601 caaagaccat gacggtgatt ataaagatca tgatatcgat tacaaggatg acgatgacaa
     3661 gtagtaagcg gccgcactcg agatatctag acccagcttt cttgtacaaa gtggttgata
     3721 tccagcacag tggcggccgc tcgagtctag agggcccgcg gttcgaaggt aagcctatcc
     3781 ctaaccctct cctcggtctc gattctacgc gtaccggtta gtaatgatcg acaatcaacc
     3841 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     3901 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     3961 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     4021 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     4081 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     4141 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     4201 tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt
     4261 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     4321 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     4381 ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggcgatg gtaccggtgt
     4441 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     4501 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     4561 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     4621 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     4681 gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     4741 ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga tcagcaccta
     4801 gcatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     4861 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
     4921 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     4981 ccctcgtgac caccttcacc tacggcgtgc agtgcttcgc ccgctacccc gaccacatga
     5041 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     5101 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     5161 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     5221 acaagctgga gtacaactac aacagccaca aggtctatat caccgccgac aagcagaaga
     5281 acggcatcaa ggtgaacttc aagacccgcc acaacatcga ggacggcagc gtgcagctcg
     5341 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     5401 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
     5461 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt
     5521 aaggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     5581 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct
     5641 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     5701 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     5761 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     5821 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
     5881 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     5941 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     6001 aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca
     6061 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     6121 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     6181 gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg
     6241 ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
     6301 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
     6361 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
     6421 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     6481 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     6541 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     6601 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     6661 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     6721 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     6781 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     6841 attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     6901 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     6961 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     7021 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     7081 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     7141 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     7201 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     7261 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     7321 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     7381 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     7441 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     7501 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     7561 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     7621 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     7681 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     7741 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     7801 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     7861 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     7921 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     7981 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     8041 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     8101 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     8161 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     8221 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     8281 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     8341 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     8401 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     8461 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     8521 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     8581 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     8641 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     8701 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     8761 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     8821 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     8881 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
     8941 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
     9001 agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg
     9061 gagctgcaag ctt
//
