LOCUS       sequence-351335-        8800 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses KSHV ORF 8 gB GQ994935 tagged.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8800)
  AUTHORS   Alzhanova D, Corcoran K, Bailey AG, Long K, Taft-Benz S, Graham RL, 
            Broussard GS, Heise M, Neumann G, Halfmann P, Kawaoka Y, Baric RS, 
            Damania B, Dittmer DP
  TITLE     Novel modulators of p53-signaling encoded by unknown genes of 
            emerging viruses.
  JOURNAL   PLoS Pathog. 2021 Jan 7;17(1):e1009033. doi: 
            10.1371/journal.ppat.1009033. eCollection 2021 Jan.
  PUBMED    33411764
REFERENCE   2  (bases 1 to 8800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Pathog."; date: "2021-01-7"; volume: "17(1):e1009033. doi"; pages: "
            10.1371/journal.ppat.1009033. eCollection 2021 Jan"
COMMENT     Sequence Label: pOME0116
FEATURES             Location/Qualifiers
     source          1..8800
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    914..938
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3518..3583
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(3613..3637)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(3648..3650,3651..3653,3654..4367)
                     /codon_start=1
                     /product="Cycle 3 GFP (Crameri et al., 1996)"
                     /label=Cycle 3 GFP
                     /note="mammalian codon-optimized"
                     /translation="MASKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(3681..3709)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     4304..4324
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(4389..4406)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4395..4619
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4665..5093
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4752..4771)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4962..4983
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5102..5122)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5107..5436
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5287..5422
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5349..5368
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5503..6297
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5557..5576)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6167..6186
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6473..6594
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6510..6529)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6564..6583
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6643..6659)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6643..6659)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6656..6678)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6667..6683
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6691..6697,6698..6715,6716..6721))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6736..6757
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6874..6891)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7045..7633)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7125..7144)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7804..8595,8596..8664))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8427..8446
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8665..8769)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt
      901 taagcttgat caaacaagtt tgtacaaaaa agcaggctct ttaaaggaac caattcagtc
      961 gactggatcc ggtaccgcca ccatgactcc caggtctaga ttggccaccc tggggactgt
     1021 catcctgttg gtctgctttt gcgcaggcgc ggcgcactcg aggggtgaca cctttcagac
     1081 gtccagttcc cccacacccc caggatcttc ctctaaggcc cccaccaaac ctggtgagga
     1141 agcatctggt cctaagagtg tggactttta ccagttcaga gtgtgtagtg catcgatcac
     1201 cggggagctt tttcggttca acctggagca gacgtgccca gacaccaaag acaagtacca
     1261 ccaagaagga attttactgg tgtacaaaaa aaacatagtg cctcatatct ttaaggtgcg
     1321 gcgctatagg aaaattgcca cctctgtcac ggtctacagg ggcttgacag agtccgccat
     1381 caccaacaag tatgaactcc cgagacccgt gccactctat gagataagcc acatggacag
     1441 cacctatcag tgctttagtt ccatgaaggt aaatgtcaac ggggtagaaa acacatttac
     1501 tgacagagac gatgttaaca ccacagtatt cctccaacca gtagaggggc ttacggataa
     1561 cattcaaagg tactttagcc agccggtcat ctacgcggaa cccggctggt ttcccggcat
     1621 atacagagtt aggaccaccg tcaattgcga gatagtggac atgatagcca ggtctgctga
     1681 accatacaat tactttgtca cgtcactggg tgacacggtg gaagtctccc ctttttgcta
     1741 taacgaatcc tcatgcagca caacccccag caacaaaaat ggccttagcg tccaagtagt
     1801 tctcaaccac actgtggtca cgtactctga cagaggaacc agtcccactc cccaaaacag
     1861 gatctttgtg gaaacgggag cgtacacgct ttcgtgggcc tccgagagca agaccacggc
     1921 cgtgtgtccg ctggcactgt ggaaaacctt cccgcgctcc atccagacta cccacgagga
     1981 cagcttccac tttgtggcca acgagatcac ggccaccttc acggctcctc taacgccagt
     2041 ggccaacttt accgacacgt actcttgtct gacctcggat atcaacacca cgctaaacgc
     2101 cagcaaggcc aaactggcga gcactcacgt ccctaacggg acggtccagt acttccacac
     2161 aacaggcgga ctctatttgg tctggcagcc catgtccgcg attaacctga ctcacgctca
     2221 gggcgacagc gggaacccca cgtcatcgcc gcccccctcc gcatccccca tgaccacctc
     2281 tgccagccgc agaaagagac ggtcagccag taccgctgct gccggcggcg gggggtccac
     2341 ggacaacctg tcttacacgc agctgcagtt tgcctacgac aaactgcggg atggcattaa
     2401 tcaggtgtta gaagaactct ccagggcatg gtgtcgcgag caggtcaggg acaacctaat
     2461 gtggtacgag ctcagtaaaa tcaaccccac cagcgttatg acagccatct acggtcgacc
     2521 tgtatccgcc aagttcgtag gagacgccat ttccgtgacc gagtgcatta acgtggacca
     2581 gagctccgta aacatccaca agagcctcag aaccaatagt aaggacgtgt gttacgcgcg
     2641 ccccctggtg acgtttaagt ttttgaacag ttccaaccta ttcaccggcc agctgggcgc
     2701 gcgcaatgag ataatactga ccaacaacca ggtggaaacc tgcaaagaca cctgcgaaca
     2761 ctacttcatc acccgcaacg agactctggt gtataaggac tacgcgtacc tgcgcactat
     2821 aaacaccact gacatatcca ccctgaacac ttttatcgcc ctgaatctat cctttattca
     2881 aaacatagac ttcaaggcca tcgagctgta cagcagtgca gagaaacgac tcgcgagtag
     2941 cgtgtttgac ctggagacga tgttcaggga gtacaactac tacacacatc gtctcgcggg
     3001 tttgcgcgag gatctggaca acaccataga tatgaacaag gagcgcttcg taagggactt
     3061 gtcggagata gtggcggacc tgggtggcat cggaaaaacg gtggtgaacg tggccagcag
     3121 cgtggtcact ctatgtggct cattggttac cggattcata aattttatta aacaccccct
     3181 aggtggcatg ctgatgatca ttatcgttat agcaatcatc ctgatcattt ttatgctcag
     3241 tcgccgcacc aataccatag cccaggcgcc ggtgaagatg atctaccccg acgtagatcg
     3301 cagggcacct cctagcggcg gagccccaac acgggaggaa atcaaaaaca tcctgctggg
     3361 aatgcaccag ctacaacaag aggagaggca gaaggcggat gatatgaaaa aaagtacacc
     3421 ctcggtgttt cagcgtaccg caaacggcct tcgtcagcgt ctgagaggat ataaacctct
     3481 gactcaatcg ctagacatca gtccggaaac gggggaggac tacaaagacc atgacggtga
     3541 ttataaagat catgatatcg attacaagga tgacgatgac aagtagtaag cggccgcact
     3601 cgagatatct agacccagct ttcttgtaca aagtggttcg atctagaatg gctagcaaag
     3661 gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt gatgttaatg
     3721 ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc tacatacgga aagcttaccc
     3781 ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt gtcactactt
     3841 tctcttatgg tgttcaatgc ttttcccgtt atccggatca tatgaaacgg catgactttt
     3901 tcaagagtgc catgcccgaa ggttatgtac aggaacgcac tatatctttc aaagatgacg
     3961 ggaactacaa gacgcgtgct gaagtcaagt ttgaaggtga tacccttgtt aatcgtatcg
     4021 agttaaaagg tattgatttt aaagaagatg gaaacattct cggacacaaa ctcgagtaca
     4081 actataactc acacaatgta tacatcacgg cagacaaaca aaagaatgga atcaaagcta
     4141 acttcaaaat tcgccacaac attgaagatg gatccgttca actagcagac cattatcaac
     4201 aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac ctgtcgacac
     4261 aatctgccct ttcgaaagat cccaacgaaa agcgtgacca catggtcctt cttgagtttg
     4321 taactgctgc tgggattaca catggcatgg atgagctcta caaataatga attaaacccg
     4381 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
     4441 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
     4501 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
     4561 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc
     4621 ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg
     4681 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     4741 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     4801 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     4861 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     4921 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     4981 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     5041 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg
     5101 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     5161 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     5221 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     5281 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     5341 atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag
     5401 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc
     5461 attttcggat ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga
     5521 ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa
     5581 cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt
     5641 ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg
     5701 ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa
     5761 gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac
     5821 cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt
     5881 gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact
     5941 cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg
     6001 ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg
     6061 acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc
     6121 atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt
     6181 gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc
     6241 gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg
     6301 ggactctggg gttcgcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt
     6361 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg
     6421 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt
     6481 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     6541 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     6601 ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt
     6661 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     6721 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     6781 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     6841 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     6901 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     6961 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     7021 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     7081 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     7141 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     7201 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     7261 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     7321 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     7381 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     7441 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     7501 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     7561 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     7621 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     7681 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     7741 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     7801 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     7861 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     7921 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     7981 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     8041 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     8101 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     8161 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     8221 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     8281 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     8341 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     8401 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     8461 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     8521 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     8581 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     8641 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     8701 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     8761 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
//
