LOCUS       sequence-351344-       12623 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for  NPC1 expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12623)
  AUTHORS   Davis OB, Shin HR, Lim CY, Wu EY, Kukurugya M, Maher CF, Perera RM, 
            Ordonez MP, Zoncu R
  TITLE     NPC1-mTORC1 Signaling Couples Cholesterol Sensing to Organelle 
            Homeostasis and Is a Targetable Pathway in Niemann-Pick Type C.
  JOURNAL   Dev Cell. 2020 Dec 7. pii: S1534-5807(20)30925-4. doi: 
            10.1016/j.devcel.2020.11.016.
  PUBMED    33308480
REFERENCE   2  (bases 1 to 12623)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Cell. 
            2020 Dec 7. pii: S1534-5807(20)30925-4. doi: 
            10.1016/j.devcel.2020.11.016."
COMMENT     Sequence Label: pLVX-NPC1(WT)-FLAG
FEATURES             Location/Qualifiers
     source          1..12623
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     315..338
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     CDS             complement(403..426)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(4332..5514)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4341..5283
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(4364..4384)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    5708..5823
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     CDS             complement(6086..6130)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6315..6548
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    7045..7170
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             7217..7850
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    8018..8152
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8042..8061)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8096..8115
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        8200..8304
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8305..8373,8374..9165)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8523..8542)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9336..9924
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9825..9844
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10078..10095
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10212..10233
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10248..10253,10254..10271,10272..10278)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10286..10302
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10291..10313
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10310..10326
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10310..10326
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     LTR             10455..11088
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    11295..11883
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             11401..11412
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     11810..11830
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(11897..12496)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(11980..12000)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     12477..12496
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     complement(12565..12584)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
ORIGIN
        1 cccccggacc caccccttcc cagcctctga gcccagaaag cgaaggagca aagctgctat
       61 tggccgctgc cccaaaggcc tacccgcttc cattgctcag cggtgctgtc catctgcacg
      121 agactagtga gacgtgctac ttccatttgt cacgtcctgc acgacgcgag ctgcggggcg
      181 ggggggaact tcctgactag gggaggagta gaaggtggcg cgaaggggcc accaaagaac
      241 ggagccggtt ggcgcctacc ggtggatgtg gaatgtgtgc gaggccagag gccacttgtg
      301 tagcgccaag tgcccagcgg ggctgctaaa gcgcatgctc cagactgcct tgggaaaagc
      361 gcctccccta cccggtagaa ttatctagat gagcccgggt tatttatcat cgtcatcctt
      421 gtagtcaaag ttcagcagtc tctctcgctc agtccccttg tatctttcct cagtggcgca
      481 gctttttgcc ttgttcacgc tagggccgat atatgacagc aggacgggca ggaaaatcag
      541 cccgtgtgtt gcgcccagca gcaccatagc caggtacatc cggaaataaa agatctggaa
      601 gatctggctc ttagcaaagg ccaggaccac gattcccccg aactttgtca gggtaatgcc
      661 actaaacact gaacttccca tgtgtgccag agcttcctct gctctttcca cccggcttcc
      721 cttcattgag acggtgaaag ctctagtgat atggctacaa aactccacac taatcccgca
      781 agacatgacc agattcacca gactgacggc gttcagagag atcccccaca gccacatcac
      841 gccgaacata ttcaccagga ccatagcgat ggtggcgcac ataatcacgg cagaccacag
      901 ctcgcagccc agcagcacca tagtgaccag gaagatagct cccagggaca cccccaggtt
      961 gaagatggta tcgtcaatga tagtcaggta ctgttcatag aacacgtaaa agacagagta
     1021 gggaaacact cgataggcgg atccattgat ccccatagtc tctgtcacgt tgctagcgat
     1081 caggcgggct ttcttcagtg catcaatgaa gtcagctgag gtctgcagca cagtgtggta
     1141 tgtcatgaag tatgtggctc ccactcgggt cccatgtccc agcaggatgt tgactgcaga
     1201 ggagtatgca gcgtggcctc ctttgccaca cttgggattt gggttgtcgg acaggaacat
     1261 aggcaggaat ctcatgaagt cgcctccctg tggtcgctgt ttgccctctg gagtcagtgg
     1321 cctacatctc acgcaggcag ggtcgaccac gcttgcattg caaaactgat ctgtgatgtt
     1381 gtccacgcga cagcagcttg actgaggctt gacccaatcg aagtaatcgt cgatccaact
     1441 agagggggca aagccaatcc ttgtataatt gtccagctgg gctgcgttga agatctgctg
     1501 caccagggaa tcattgttac atcccattcc cccgcacacc atattctgcc ccttggagct
     1561 agtgtagtca tggccttcct ccagcacgaa gtagacaggg gggccggcgt gcagatactg
     1621 actgatagat ttaaagtaat ccaccatgta agaatcgtcg ggcatggaca ggctctgatc
     1681 caggccgatg tcgaccttgt tcagcacggc aattgagaaa ctcagcactc cgacaaagat
     1741 tgcaatcacg atggggcgca tccagtcttt cagcagcagt ggagaatagc tgttcttgaa
     1801 aaacctgaac agacagcttt ctgaagcctg cacggatgtg ccgtcctctg ctccccgcac
     1861 gcagcagaag atatccaggc ggtttttctc ctgcctctta atgtccaggc ccagcaggct
     1921 cacaaagcag gtgatctgca gcaggaagtc aataaacact gccagcccag cgaacagtga
     1981 aaatgtgtgg actgctggca tcacactcag ggcgcccagg aaaaatgcca cggtttcact
     2041 gaatgaactc agaaacatag atggagcgac ctctcccagc acccttccca gctgctgatc
     2101 cagtgtttcg ccctgcagtc gctcgtcgcg ctggtatgcc tgcaccagaa tgaagatatt
     2161 gtccactccg acagccagga ccagaaatgg gatcacttca atgacgatca gggtcagagg
     2221 cagtccaata taggagaaca cccccaggct acaagcgaca gaggacagca caatcaggat
     2281 gccggcaatt cccaggctga cttttgaatc caccagcagg cgcctgcagc tcttcatatg
     2341 gcccagtgcc agtgagatgt acaggaacat gatagcatag ctaatgacca cggtgaacac
     2401 gtcactgtca gattctcggt tcagttcgtc ctcgatgctc cgctcggctg tgaaactaat
     2461 ggtcagattt gggtttttgt aattcttcac gaagttgatg aactctttct cccaggcctg
     2521 tgctctctgc agcttctcgg tatcattata gtaattgttg acagggaagg tgatgaccag
     2581 ggcggtggca ttgttgtagt tctgatcgtc atagcctccc aggaccagcc aagggaacac
     2641 gggtccgcca aatgtcccca gacatggatc gtgcagcagt gaggtgtcgt tcagacttgc
     2701 aggagcccgc acgcagtaca ggaaatgggt gtgatagtcg gcgtacacga agaagtcgtc
     2761 gcctttctta tgatccagca cggagtggct attctgaaag tagttcagga cggacagaat
     2821 ggtacaatta gtgttatatg ggctcagagg agccaggcag atatcctgca gggtcacagt
     2881 ttcattgtca tagctagcgg taatgttctc aatggcgatc tgcaggtcca gcacctgatg
     2941 cagaatctgg atatccagtg gagggccgaa aggcacgtcg gctccagaag gatagggctg
     3001 gtagatgtgt ttatcagtca ggggtgccct aatgatcagc tgctctgttc tgaaaaatgg
     3061 tccgaaatgc tggtcaaagt attccttctc cagtcgagcc tggcttgatg gggcgctcca
     3121 cagatccact ggatttgtgg tcactcggac gaacaccagc ccactagaac aggcggtgat
     3181 aaacaccagg ctgaagaaga tcacgcatcc ggggttccgc acgcagaaag acccccatcg
     3241 agtaaacagt ctccgcaggc acccttcgaa agcggcgctc acgggatcac agcaggatgc
     3301 ctcgcctttg tcggatgcat tcacgctgaa agcgatgttg gagtcaatgg gggtgtactc
     3361 gctcacaaag taccgctttc gataacacca cacggcgaaa aatgcgccga aaaagaccag
     3421 cagaaaggcc atgtaagtga tccacataat cacatacatg gcgtccagtc ccagaattgt
     3481 ccatggtgct ggtggaggtg gtggctgtgg tttaggcccg cacacgatag agcaatcctg
     3541 acaggagcaa ggagcggtca cttcatcgac ggactcgtcg catcccttag tggcattgtt
     3601 cataggctcc atcccgtgga cggggaagtc gctaaacact ggtgtaatgg tgaaaggggc
     3661 ctggccatta tccttgttaa acatgtattc gatccaattg gtagcgttac aggcatctgc
     3721 gtcttttccg cacagcagcc ccagggcctt atcattggag cttggtgcct ccacgtctcg
     3781 gcaagcatta tacatagcgt tggcgaagga ctggcccaca tagtactgca gttctttgac
     3841 attagtcttt gtctggtttg tcacgggatc gacgtagtcc tcggtggcag tcacgttcag
     3901 gaactgtgac tgccttggac tacaggtcag ctcgcagaac agattcagca ggttataaaa
     3961 acaagatggg cacctggaca gaaactgcag aggcagctgc agattatcct tcagggtctg
     4021 cagctgccgc acgtcacagc acaggctcac gttgccgaaa aagaatcctg ggcacagttc
     4081 ctgcaccaga tcgtagccgt ctttgggcag tggcttaggg ggtccagagt attcacagtt
     4141 atacctcttg tccccatagg cgatgccgca ctcgccatac cacacgcaag actgactgaa
     4201 cacctgagcg gggcacagca gcagcagcag cagccccaga gccagccctc tagcggtcat
     4261 ggtggcgaat tccacgtgga tccaattcga agcttgagct cgagatctga gtccggtagc
     4321 gctagcgatc ctcacgacac ctgaaatgga agaaaaaaac tttgaaccac tgtctgaggc
     4381 ttgagaatga accaagatcc aaactcaaaa agggcaaatt ccaaggagaa ttacatcaag
     4441 tgccaagctg gcctaacttc agtctccacc cactcagtgt ggggaaactc catcgcataa
     4501 aacccctccc cccaacctaa agacgacgta ctccaaaagc tcgagaacta atcgaggtgc
     4561 ctggacggcg cccggtactc cgtggagtca catgaagcga cggctgagga cggaaaggcc
     4621 cttttccttt gtgtgggtga ctcacccgcc cgctctcccg agcgccgcgt cctccatttt
     4681 gagctccctg cagcagggcc gggaagcggc catctttccg ctcacgcaac tggtgccgac
     4741 cgggccagcc ttgccgccca gggcggggcg atacacggcg gcgcgaggcc aggcaccaga
     4801 gcaggccggc cagcttgaga ctacccccgt ccgattctcg gtggccgcgc tcgcaggccc
     4861 cgcctcgccg aacatgtgcg ctgggacgca cgggccccgt cgccgcccgc ggccccaaaa
     4921 accgaaatac cagtgtgcag atcttggccc gcatttacaa gactatcttg ccagaaaaaa
     4981 agcgtcgcag caggtcatca aaaattttaa atggctagag acttatcgaa agcagcgaga
     5041 caggcgcgaa ggtgccacca gattcgcacg cggcggcccc agcgcccaag ccaggcctca
     5101 actcaagcac gaggcgaagg ggctccttaa gcgcaaggcc tcgaactctc ccacccactt
     5161 ccaacccgaa gctcgggatc aagaatcacg tactgcagcc aggggcgtgg aagtaattca
     5221 aggcacgcaa gggccataac ccgtaaagag gccaggcccg cgggaaccac acacggcact
     5281 tacctgtgtt ctggcggcaa acccgttgcg aaaaagaacg ttcacggcga ctactgcact
     5341 tatatacggt tctcccccac cctcgggaaa aaggcggagc cagtacacga catcactttc
     5401 ccagtttacc ccgcgccacc ttctctaggc accggttcaa ttgccgaccc ctccccccaa
     5461 cttctcgggg actgtgggcg atgtgcgctc tgcccactga cgggcaccgg agcctcacgc
     5521 atgctcttct ccacctcagt gatgacgaga gcgggcgggt gagggggcgg gaacgcagcg
     5581 atctctgggt tctacgttag tgggagttta acgacggtcc ctgggattcc ccaaggcagg
     5641 ggcgagtcct tttgtatgaa ttactcatcg ataaactgga tctctgctgt ccctgtaata
     5701 aacccgaaaa ttttgaattt ttgtaatttg tttttgtaat tctttagttt gtatgtctgt
     5761 tgctattatg tctactattc tttcccctgc actgtacccc ccaatccccc cttttctttt
     5821 aaaggcgata ccgtcgagat ccgttcacta atcgaatgga tctgtctctg tctctctctc
     5881 caccttcttc ttctattcct tcgggcctgt cgggtcccct cggggttggg aggtgggtct
     5941 gaaacgataa tggtgaatat ccctgcctaa ctctattcac tatagaaagt acagcaaaaa
     6001 ctattcttaa acctaccaag cctcctacta tcattatgaa taattttata taccacagcc
     6061 aatttgttat gttaaaccaa ttccacaaac ttgcccattt atctaattcc aataattctt
     6121 gttcattctt ttcttgctgg ttttgcgatt cttcaattaa ggagtgtatt aagcttgtgt
     6181 aattgttaat ttctctgtcc cactccatcc aggtcgtgtg attccaaatc tgttccagag
     6241 atttattact ccaactagca ttccaaggca cagcagtggt gcaaatgagt tttccagagc
     6301 aaccccaaat ccccaggagc tgttgatcct ttaggtatct ttccacagcc aggattcttg
     6361 cctggagctg cttgatgccc cagactgtga gttgcaacag atgctgttgc gcctcaatag
     6421 ccctcagcaa attgttctgc tgctgcacta taccagacaa taattgtctg gcctgtaccg
     6481 tcagcgtcat tgacgctgcg cccatagtgc ttcctgctgc tcccaagaac ccaaggaaca
     6541 aagctcctat tcccactgct cttttttctc tctgcaccac tcttctcttt gccttggtgg
     6601 gtgctactcc taatggttca atttttacta ctttatattt atataattca cttctccaat
     6661 tgtccctcat atctcctcct ccaggtctga agatcagcgg ccggccgctt gctgtgcggt
     6721 ggtcttactt ttgttttgct cttcctctat cttgtctaaa gcttccttgg tgtcttttat
     6781 ctctatcctt tgatgcacac aatagagggt tgctactgta ttatataatg atctaagttc
     6841 ttctgatcct gtctgaaggg atggttgtag ctgtcccagt atttgtctac agccttctga
     6901 tgtttctaac aggccaggat taactgcgaa tcgttctagc tccctgcttg cccatactat
     6961 atgttttaat ttatattttt tctttccccc tggccttaac cgaatttttt cccatcgcga
     7021 tctaattctc ccccgcttaa tactgacgct ctcgcaccca tctctctcct tctagcctcc
     7081 gctagtcaaa atttttggcg tactcaccag tcgccgcccc tcgcctcttg ccgtgcgcgc
     7141 ttcagcaagc cgagtcctgc gtcgagagag ctcctctggt ttccctttcg ctttcaagtc
     7201 cctgttcggg cgccactgct agagattttc cacactgact aaaagggtct gagggatctc
     7261 tagttaccag agtcacacaa cagacgggca cacactactt gaagcactca aggcaagctt
     7321 tattgaggct taagcagtgg gttccctagt tagccagaga gctcccaggc tcagatctgg
     7381 tctaaccaga gagacccagt acaggcaaaa agcagctgct tatatgcagg atctgagggc
     7441 tcgccactcc ccagtcccgc ccaggccacg cctccctgga aagtccccag cggaaagtcc
     7501 cttgtagcaa gctcgatatc agcagttctt gaagtactcc ggatgcagct ctcgggccac
     7561 gtgatgaaat gctaggcggc tgtcaaacct ccactctaac acttctctct ccgggtcatc
     7621 catcccatgc aggctcacag ggtgtaacaa gctggtgttc tctcctttat tggcctcttc
     7681 taccttatct ggctcaactg gtactagctt gtagcaccat ccaaaggtca gtggatatct
     7741 gacccctggc cctggtgtgt agttctgcta atcagggaag tagccttgtg tgtggtagat
     7801 ccacagatca aggatatctt gtcttctttg ggagtgaatt agcccttcca actactaagt
     7861 ttgtagtaca tatttaacaa atacaatttc tttaaaatga aaataattca gaggaatcac
     7921 aggtttagag taaatgaaac cacaggtaat tggcagtggt aatagggtat ggggtgggaa
     7981 gtttgggatg attttggtta gcttgagtta tccagttgat ccagacatga taagatacat
     8041 tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat
     8101 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttgatctccc
     8161 gatccgtcga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     8221 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     8281 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     8341 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     8401 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     8461 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     8521 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     8581 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     8641 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     8701 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     8761 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     8821 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     8881 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     8941 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     9001 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     9061 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     9121 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     9181 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     9241 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     9301 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     9361 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     9421 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     9481 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     9541 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     9601 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     9661 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     9721 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     9781 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     9841 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     9901 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     9961 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
    10021 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
    10081 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
    10141 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
    10201 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
    10261 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
    10321 tatgaccatg attacgccaa gctctagcta gaggtcgacg gtaaaacgct agcaatgtca
    10381 aggcctctca ctctctgata ttcatttctt tgcaagttat aaatactgaa taataagatg
    10441 acatgaacta ctactgctag agattttcca cactgactaa aagggtctga gggatctcta
    10501 gttaccagag tcacacaaca gacgggcaca cactacttga agcactcaag gcaagcttta
    10561 ttgaggctta agcagtgggt tccctagtta gccagagagc tcccaggctc agatctggtc
    10621 taaccagaga gacccagtac aggcaaaaag cagctgctta tatgcaggat ctgagggctc
    10681 gccactcccc agtcccgccc aggccacgcc tccctggaaa gtccccagcg gaaagtccct
    10741 tgtagcaagc tcgatatcag cagttcttga agtactccgg atgcagctct cgggccacgt
    10801 gatgaaatgc taggcggctg tcaaacctcc actctaacac ttctctctcc gggtcatcca
    10861 tcccatgcag gctcacaggg tgtaacaagc tggtgttctc tcctttattg gcctcttcta
    10921 ccttatctgg ctcaactggt actagcttgt agcaccatcc aaaggtcagt ggatatctga
    10981 cccctggccc tggtgtgtag ttctgctaat cagggaagta gccttgtgtg tggtagatcc
    11041 acagatcaag gatatcttgt cttcgttggg agtgaattag cccttccagt cccctctttt
    11101 cttttaaaaa gtggctaaga tctacagctg ccttgtaagt cattggtctt aaaggtacct
    11161 gaggtgtgac tggaaaaccc acctcctcct cctcttgtgc ttctagccag gcacaatcag
    11221 cattggtagc tgctgtattg ctacttgtga ttgctccatg tttttctagg tctcgactgc
    11281 agaattaatt ccaggcgggg aggcggccca aagggagatc cgactcgtct gagggcgaag
    11341 gcgaagacgc ggaagaggcc gcagagccgg cagcaggccg cgggaaggaa ggtccgctgg
    11401 attgagggcc gaagggacgt agcagaagga cgtcccgcgc agaatccagg tggcaacaca
    11461 ggcgagcagc catggaaagg acgtcagctt ccccgacaac accacggaat tgtcagtgcc
    11521 caacagccga gcccctgtcc agcagcgggc aaggcaggcg gcgatgagtt ccgccgtggc
    11581 aatagggagg gggaaagcga aagtcccgga aaggagctga caggtggtgg caatgcccca
    11641 accagtgggg gttgcgtcag caaacacagt gcacaccacg ccacgttgcc tgacaacggg
    11701 ccacaactcc tcataaagag acagcaacca ggatttatac aaggaggaga aaatgaaagc
    11761 catacgggaa gcaatagcat gatacaaagg cattaaagca gcgtatccac atagcgtaaa
    11821 aggagcaaca tagttaagaa taccagtcaa tctttcacaa attttgtaat ccagaggttg
    11881 attgttccag acgcggtcag gcaccgggct tgcgggtcat gcaccaggtg cgcggtcctt
    11941 cgggcacctc gacgtcggcg gtgacggtga agccgagccg ctcgtagaag gggaggttgc
    12001 ggggcgcgga ggtctccagg aaggcgggca ccccggcgcg ctcggccgcc tccactccgg
    12061 ggagcacgac ggcgctgccc agacccttgc cctggtggtc gggcgagacg ccgacggtgg
    12121 ccaggaacca cgcgggctcc ttgggccggt gcggcgccag gaggccttcc atctgttgct
    12181 gcgcggccag ccgggaaccg ctcaactcgg ccatgcgcgg gccgatctcg gcgaacaccg
    12241 cccccgcttc gacgctctcc ggcgtggtcc agaccgccac cgcggcgccg tcgtccgcga
    12301 cccacacctt gccgatgtcg agcccgacgc gcgtgaggaa gagttcttgc agctcggtga
    12361 cccgctcgat gtggcggtcc ggatcgacgg tgtggcgcgt ggcggggtag tcggcgaacg
    12421 cggcggcgag ggtgcgtacg gccctgggga cgtcgtcgcg ggtggcgagg cgcaccgtgg
    12481 gcttgtactc ggtcatggta agcttgggct gcaggtcgaa aggcccggag atgaggaaga
    12541 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
    12601 ccttcgggcg cccgccccgc ccc
//
